Effects of histidine and imidazolelactic acid on various parameters of the oxidative stress in cerebral cortex of young rats

Abstract

Histidinemia is an inherited metabolic disorder caused by deficiency of histidase activity, which leads to tissue accumulation of histidine and its derivatives. Affected patients usually present with speech delay and mental retardation, although asymptomatic patients have been reported. Considering that the pathophysiology of the neurological dysfunction of histidinemia is not yet understood and since histidine has been considered a pro-oxidant agent, in the present study we investigated the effect of histidine and one of its derivatives, l-beta-imidazolelactic acid, at concentrations ranging from 0.1 to 10 mM, on various parameters of oxidative stress in cerebral cortex of 30-day-old Wistar rats. Chemiluminescence, total radical-trapping antioxidant potential (TRAP), thiobarbituric acid reactive substances (TBA-RS), and the activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were measured in tissue homogenates in the presence of l-histidine or l-beta-imidazolelactic acid. We observed that l-histidine provoked an increase of chemiluminescence and a reduction of TRAP at concentrations of 2.5 mM and higher, while TBA-RS measurement, GSH-Px, CAT and SOD activities were not affected. Furthermore, l-beta-imidazolelactic acid provoked antioxidant effects at high concentrations (5-10 mM) as observed by the reduction of chemiluminescence, although this compound enhanced chemiluminescence at low concentrations (0.5-1 mM). These results suggest that in vitro oxidative stress is elicited by histidine but only at supraphysiological concentrations.

Heat Stress Affects Faecal Microbial and Metabolic Alterations of Rabbits

“…Furthermore, Borton et al (2017) found that low levels of inflammation increased the relative number of these bacteria in the mouse stomach. It is worth noting that Akkermansia is positively correlated with imidazolectic acid, an antioxidant effect ( Tansini et al, 2004 ). Therefore, we speculate that rabbits exposed to HS have a low immune function, anti-inflammatory response, and the destruction of intestinal mucosal tissue, whereas Akkermansia positively regulates the production of antioxidant metabolites, protects the self-healing of the intestinal mucosal protective layer, and enhances the response to inflammatory reaction damage.…”

The Potential Role of Citrus limon Powder as a Natural Feed Supplement to Boost the Productive Performance, Antioxidant Status, and Blood Biochemistry of Growing Rabbits

“…However, previous reports have placed more emphasis on the antioxidant capacity of C. limon [4,57,58]. C. limon also contains several constituents such as 3-carene, imidazole, D-limonene, geraniol, and squalene as well as other compounds reported as potent antioxidants that can prevent microsomal lipid peroxide and protein degradation of ROS [59,60,61,62,63], These data are in accordance with our phytochemical study results which highlighted C. limon as a powerful scavenging agent.…”

Metabonomics analysis of kidneys in rats administered with chronic low‐dose cadmium by ultra‐performance liquid chromatography‐mass spectrometry

“…In this study, the intensity of imidazole lactic acid was significantly reduced in rat renal tissues in the high‐dose group, showing that Cd may affect the metabolism of histidine. Some studies have reported that imidazole lactic acid also has an antioxidant effect (Oya & Yamamoto, ; Tansini et al, ). In the current study, the significantly reduced intensity of imidazole lactic acid in kidneys may be associated with Cd‐induced oxidative stress.…”