Effects of hyaluronic acid on differentiation of human amniotic epithelial cells and cell-replacement therapy in type 1 diabetic mice

Luo, Yi; Cheng, Yawei; Yu, Chunhao; Liu, Ru‐Ming; Zhao, Yujie; Chen, Daixiong; Zhong, Jian‐Jiang; Xiao, Jian‐Hui

doi:10.1016/j.yexcr.2019.111642

Cited by 12 publications

(22 citation statements)

References 37 publications

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“…Non-immune isotype IgG antibodies (BD, NJ, USA) were used as controls. Similar to our previous study (Luo et al, 2019), hAECs highly expressed the surface markers such as CD29 (99.62%), CD73 (99.60%), and CD166 (99.39%), and lowly expressed CD44 (17.6%), but did not express the hematopoietic stem cells marker CD34 (0.09%) and CD45 (1.17%), and the MHC II molecule HLA-DR (0.02%). The absence of CD34 and CD45 positive cells indicated that the isolates were not contaminated with hematopoietic stem cells from umbilical cord blood or embryonic fibroblasts.…”

Section: Phenotypic Properties Of Haecssupporting

confidence: 87%

“…Unlike human ESCs, hAECs do not express telomerase and thus are not tumorigenic upon transplantation (Miki, 2018). Based on these excellent properties, hAECs have been widely applied in the pre-clinical treatment of many diseases, e.g., liver cirrhosis (Lin et al, 2015), pulmonary damages (Zhu et al, 2017), neurological disorders (Sankar & Muthusamy, 2003), autoimmune uveitis (Li et al, 2018), diabetes (Luo et al, 2019), early Achilles tendon defects (Barboni et al, 2018), as well as aging-related diseases (Di Germanio et al, 2016). Therefore, hAECs possess considerable therapeutic potentials in regenerative medicine.…”

Section: Introductionmentioning

confidence: 99%

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Hyaluronic acid ameliorates the proliferative ability of human amniotic epithelial cells through activation of TGF-β/BMP signaling

Tian

Wang

et al. 2020

PeerJ

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Human amniotic epithelial cells (hAECs) are a useful and noncontroversial source of stem cells for cell therapy and regenerative medicine, but their limited proliferative ability hinders the acquisition of adequate quantities of cells for clinical use due to not expressing telomerase in hAECs. Our previous study showed that hyaluronic acid (HA), an important component of the extracellular matrix, promoted the proliferation of human amniotic mesenchymal stem cells. Herein, we hypothesize that HA might improve the proliferative capability of hAECs. In the present study, the role of HA on the proliferation of human amniotic epithelial cells (hAECs) in vitro was investigated for the first time. HA at molecular weight of 300 kDa showed an obvious pro-proliferation effect on hAECs. Furthermore, HA not only kept phenotypic characteristics and differentiation capabilities of hAECs, but significantly promoted the secretion of the anti-inflammatory factors such as IL-10 and TGF-β1, and the expression of stem cell pluripotent factors such as Oct4 and Nanog. Analysis of PCR microarray data and RT-qPCR validation showed that TGF-β/BMP signaling was activated in the presence of HA. Further study showed that SB431542, an inhibitor of the TGF-β/BMP signaling, significantly suppressed the mRNA expression of TGFBR3, BMP4, BMP7, BMPR1B, SMAD3, SMAD4, and the pro-proliferative effect of HA on hAECs. These data suggest that HA is a safe and effective enhancer for in vitro expansion of hAECs, whose regulatory mechanism involves the TGF-β/BMP signaling.

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Section: Phenotypic Properties Of Haecssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Hyaluronic acid ameliorates the proliferative ability of human amniotic epithelial cells through activation of TGF-β/BMP signaling

Tian

Wang

et al. 2020

PeerJ

Self Cite

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“…hAESCs had multidirectional differentiation potential similar to embryonic stem cells and pluripotent stem cells [116]. Luo et al observed that hAESCs transplantation significantly reduced hyperglycemia symptoms, increased the plasma insulin content, and partially repaired the islet structure in type 1 diabetic mice and the combination of hAESCs with hyaluronic acid exhibited a remarkable therapeutic effect compared to hAESCs alone group, indicating that hyaluronic acid was an efficient co-inducer of the differentiation of hAESCs into functional insulin-producing cells [215]. Peng et al found that the combination of nicotinamide plus betacellulin promoted the differentiation of hAESCs into islet-like cell clusters by reducing the DNA methylation [1].…”

Section: Diabetes Mellitusmentioning

confidence: 99%

Characteristics and Therapeutic Potential of Human Amnion-Derived Stem Cells

Liu

Huang

et al. 2021

IJMS

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Stem cells including embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and adult stem cells (ASCs) are able to repair/replace damaged or degenerative tissues and improve functional recovery in experimental model and clinical trials. However, there are still many limitations and unresolved problems regarding stem cell therapy in terms of ethical barriers, immune rejection, tumorigenicity, and cell sources. By reviewing recent literatures and our related works, human amnion-derived stem cells (hADSCs) including human amniotic mesenchymal stem cells (hAMSCs) and human amniotic epithelial stem cells (hAESCs) have shown considerable advantages over other stem cells. In this review, we first described the biological characteristics and advantages of hADSCs, especially for their high pluripotency and immunomodulatory effects. Then, we summarized the therapeutic applications and recent progresses of hADSCs in treating various diseases for preclinical research and clinical trials. In addition, the possible mechanisms and the challenges of hADSCs applications have been also discussed. Finally, we highlighted the properties of hADSCs as a promising source of stem cells for cell therapy and regenerative medicine and pointed out the perspectives for the directions of hADSCs applications clinically.

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“…The second is to use the stemness characteristics of amniotic cells as a source for differentiation into insulin-producing cells. hAECs have been successfully differentiated into cells with a β cell phenotype, with the capacity to control glycemia in streptozotocin-induced diabetic mice [98,99].…”

Section: Tissue Engineering and Cell-based Therapymentioning

confidence: 99%

Immunomodulatory Properties of Amniotic Membrane Derivatives and Their Potential in Regenerative Medicine

Wassmer

Berishvili

2020

Curr Diab Rep

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Purpose of Review During the last decades, the field of regenerative medicine has been rapidly evolving. Major progress has been made in the development of biological substitutes applying the principles of cell transplantation, material science, and bioengineering. Recent Findings Among other sources, amniotic-derived products have been used for decades in various fields of medicine as a biomaterial for the wound care and tissue replacement. Moreover, human amniotic epithelial and mesenchymal cells have been intensively studied for their immunomodulatory capacities. Summary Amniotic cells possess two major characteristics that have already been widely exploited. The first is their ability to modulate and suppress the innate and adaptive immunities, making them a true asset for chronic inflammatory disorders and for the induction of tolerance in transplantation models. The second is their multilineage differentiation capacity, offering a source of cells for tissue engineering. The latter combined with the use of amniotic membrane as a scaffold offers all components necessary to create an optimal environment for cell and tissue regeneration. This review summarizes beneficial properties of hAM and its derivatives and discusses their potential in regenerative medicine.

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Effects of hyaluronic acid on differentiation of human amniotic epithelial cells and cell-replacement therapy in type 1 diabetic mice

Cited by 12 publications

References 37 publications

Hyaluronic acid ameliorates the proliferative ability of human amniotic epithelial cells through activation of TGF-β/BMP signaling

Hyaluronic acid ameliorates the proliferative ability of human amniotic epithelial cells through activation of TGF-β/BMP signaling

Characteristics and Therapeutic Potential of Human Amnion-Derived Stem Cells

Immunomodulatory Properties of Amniotic Membrane Derivatives and Their Potential in Regenerative Medicine

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