Effects of hybridization of decellularized allogenic nerves with adipose-derive stem cell sheets to facilitate nerve regeneration

Nakada, Mika; Itoh, Soichiro; Tada, Kazuhiro; Matsuta, Masashi; Murai, Atsuro; Tsuchiya, Hiroyuki

doi:10.1016/j.brainres.2020.147025

Cited by 14 publications

(16 citation statements)

References 24 publications

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“…Both Groups C and D improved nerve fiber density. It stays in line with other reports concerning the use of ASCs as an additive in nerve regeneration therapies [ 28 , 29 ]. The similarly beneficial effect of ASCs sheet enveloping a decellularized autograft was presented by Nakada et al; however, their solution did not exceed the axonal density of the comparator (1.8 vs. 2.29 axons/µm 2 ) as we observed in Group D vs. B in both stainings.…”

Section: Discussionsupporting

confidence: 91%

“…However, the addition of ASCs to the conduit slightly decreased the beneficial effect vs. the autograft group. It opposes other studies, where ASCs addition either to allografts or conduits was even more profitable for wet muscle mass ratio [ 27 , 28 , 29 ]. Yet, it was statistically significant that the differences between mean values of Groups B, C, and D remain much lower vs. similar studies.…”

Section: Discussionmentioning

confidence: 61%

“…Yet, it was statistically significant that the differences between mean values of Groups B, C, and D remain much lower vs. similar studies. Recently, Nakada et al reported the beneficial effect of enriching decellularized autografts with ASC, which enabled maintaining 60% of wet muscle mass ratio vs. the autografted group [ 28 ]. In our study, the conduit or its ASCs-enriched version achieved respectively 96% and 87% muscle mass ratio preservation with relation to the autografted group.…”

Section: Discussionmentioning

confidence: 99%

“…However, Kappos et al states that supreme regeneration conditions were provided by ASCs-SC compared to other ASC types (rat uASC, human superficial or deep uASC) [ 22 ]. Nakada along with other authors assumes that the beneficial effect of ASCs in conduits relies on the humoral effect of secreted neurotrophic factors [ 20 , 28 , 34 ]. As these studies show, undifferentiated ASCs are not viable after around 14 days after implantation [ 20 ] and do not provide myelinization, as they did in the case of processed allografts or conduits lacking living and interacting cells in their structure.…”

Section: Discussionmentioning

confidence: 99%

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Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2021

IJMS

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The aim was to examine the efficiency of a scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen (COL), polyaniline (PANI), and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model. P(LLA-CL)-COL-PANI scaffold was optimized and electrospun into a tubular-shaped structure. Adipose tissue from 10 Lewis rats was harvested for ASCs culture. A total of 28 inbred male Lewis rats underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In Group A, the nerve gap remained untouched; in Group B, an excised trunk was used as an autograft; in Group C, nerve stumps were secured with P(LLA-CL)-COL-PANI conduit; in Group D, P(LLA-CL)-COL-PANI conduit was enriched with ASCs. After 6 months of observation, rats were sacrificed. Gastrocnemius muscles and sciatic nerves were harvested for weight, histology analysis, and nerve fiber count analyses. Group A showed advanced atrophy of the muscle, and each intervention (B, C, D) prevented muscle mass decrease (p < 0.0001); however, ASCs addition decreased efficiency vs. autograft (p < 0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the groups with the use of conduit (D vs. B p < 0.0001, C vs. B p < 0.001). P(LLA-CL)-COL-PANI conduits with ASCs showed promising results in managing nerve gap by decreasing muscle atrophy.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2021

IJMS

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“…Li et al transplanted a decellularized allogenic nerve graft seeded with BMSCs to bridge a 30mm-long sciatic nerve defect in rabbits and obtained nerve regeneration similar to that in autogenous nerve grafts [ 45 ]. Nakada et al wrapped a decellularized nerve with a sheet seeded with adipose derived stem cells and transplanted it to bridge a 15mm-rat sciatic nerve defect and reported that nerve regeneration in their materials was compatible with that in autogenous nerve grafts [ 46 ]. Because of the difference of animal species and methods evaluating nerve regeneration, it was unknown which nerve induction material was the most promotive for peripheral nerve regeneration.…”

Section: Discussionmentioning

confidence: 99%

Bone marrow-derived mesenchymal stem cells transplanted into a vascularized biodegradable tube containing decellularized allogenic nerve basal laminae promoted peripheral nerve regeneration; can it be an alternative of autologous nerve graft?

et al. 2021

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Previously, we showed silicone nerve conduits containing a vascular bundle and decellularized allogenic basal laminae (DABLs) seeded with bone marrow-derived mesenchymal stem cells (BMSCs) demonstrated successful nerve regeneration. Nerve conduits should be flexible and biodegradable for clinical use. In the current study, we used nerve conduits made of polyglycoric acid (PGA) fiber mesh, which is flexible, biodegradable and capillary-permeable. DABLs were created using chemical surfactants to remove almost all cell debris. In part 1, capillary infiltration capability of the PGA tube was examined. Capillary infiltration into regenerated neural tissue was compared between the PGA tube with blood vessels attached extratubularly (extratubularly vascularized tube) and that containing blood vessels intratubularly (intratubularly vascularized tube). No significant difference was found in capillary formation or nerve regeneration between these two tubes. In part 2, a 20 mm gap created in a rat sciatic nerve model was bridged using the extratubularly vascularized PGA tube containing the DABLs with implantation of isogenic cultured BMSCs (TubeC+ group), that containing the DABLs without implantation of the BMSCs (TubeC- group), and 20 mm-long fresh autologous nerve graft (Auto group). Nerve regeneration in these three groups was assessed electrophysiologically and histomorphometrically. At 24 weeks, there was no significant difference in any electrophysiological parameters between TubeC+ and Auto groups, although all histological parameters in Auto group were significantly greater than those in TubeC+ and TubeC- groups, and TubeC+ group demonstrated significant better nerve regeneration than TubeC- group. The transplanted DABLs showed no signs of immunological rejection and some transplanted BMSCs were differentiated into cells with Schwann cell-like phenotype, which might have promoted nerve regeneration within the conduit. This study indicated that the TubeC+ nerve conduit may become an alternative to nerve autograft.

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Bioengineering approaches for nerve graft revascularization: Current concepts and future directions

Pereira

Lazar

Wang

2023

WIREs Mechanisms of Disease

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Peripheral nerve injury (PNI) is the most common neurological injury in civilian and military injuries, with over 360,000 PNI procedures performed in the US yearly. Segmental loss of nerve tissue results in a nerve gap precluding a tension-free primary repair, and in these cases, interpositional autologous or acellular nerve allografts are used to bridge the gap. Graft ischemia time is a critical factor in achieving satisfactory nerve regeneration. Rapid nerve graft revascularization is essential in order to sustain Schwann cell growth which in turn is crucial for axonal regeneration. Currently, nerve autografts are considered the gold standard for segmental nerve gaps but are associated with several disadvan-

show abstract

Effects of hybridization of decellularized allogenic nerves with adipose-derive stem cell sheets to facilitate nerve regeneration

Cited by 14 publications

References 24 publications

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Bone marrow-derived mesenchymal stem cells transplanted into a vascularized biodegradable tube containing decellularized allogenic nerve basal laminae promoted peripheral nerve regeneration; can it be an alternative of autologous nerve graft?

Bioengineering approaches for nerve graft revascularization: Current concepts and future directions

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