Effects of Hydrogel/Silver Coatings on In Vitro Adhesion to Catheters of Bacteria Associated with Urinary Tract Infections

Ahearn, Donald G.; Grace, Deanine T.; Jennings, Matthew J.; Borazjani, Roya N.; Boles, Karen J.; Rose, Laura J.; Simmons, Robert B.; Ahanotu, Ejem N.

doi:10.1007/s002840010105

Cited by 127 publications

(75 citation statements)

References 6 publications

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“…The insensitivity of biofilm bacteria to antibiotics is a function of cell wall composition, surface structure, and phenotypic variation in enzymatic activity (8,14). It has also been suggested that the negatively charged exopolysaccharide is very effective in protecting bacterial cells from cationic antibiotics by restricting their permeation (2).In the last decade, several strategies to control biofilm growth on medical devices have been suggested, including using topical antimicrobial ointments, minimizing the length of time of catheterization, using catheters provided with a surgically implanted cuff (12), and coating the catheter lumen with antimicrobial agents (1,7,9,19,26,27). Enzymes involved in bacterial cell wall synthesis could provide novel targets for the development of antibiofilm agents.…”

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confidence: 99%

Antibiofilm Activity of GlmU Enzyme Inhibitors against Catheter-Associated Uropathogens

Burton

Gawande

Yakandawala

et al. 2006

Antimicrob Agents Chemother

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The colonization of uropathogenic bacteria on urinary catheters resulting in biofilm formation frequently leads to the infection of surrounding tissue and often requires removal of the catheter. Infections associated with biofilms are difficult to treat since they may be more than 1,000 times more resistant to antibiotics than their planktonic counterparts. We have developed an antibiofilm composition comprising an N-acetyl-Dglucosamine-1-phosphate acetyltransferase (GlmU) inhibitor and protamine sulfate, a cationic polypeptide. The antibiofilm activity of GlmU inhibitors, such as iodoacetamide (IDA), N-ethyl maleimide (NEM), and NEM analogs, including N-phenyl maleimide, N,N-(1,2-phenylene)dimaleimide (oPDM), and N-(1-pyrenyl)maleimide (PyrM), was tested against that of catheter-associated uropathogens. Both IDA and NEM inhibited biofilm formation in Escherichia coli. All NEM analogs showed antibiofilm activity against clinical isolates of E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus epidermidis, and Enterococcus faecalis. The combination of oPDM with protamine sulfate (PS) enhanced its antibiofilm activity and reduced its effective concentration to as low as 12.5 M. In addition, we found that the in vitro inhibitory activity of oPDM-plus-PS-coated silicone catheters against P. aeruginosa and S. epidermidis colonization was superior to that of catheters coated with silver hydrogel. Confocal scanning laser microscopy further confirmed that the oPDMplus-PS-coated silicone catheters were almost free from bacterial colonization. Thus, a broad-spectrum antibiofilm composition comprising a GlmU inhibitor and protamine sulfate shows promise for use in antiinfective coatings for medical devices, including urinary catheters.Microorganisms can attach to and colonize any biomaterial surface, putting patients at risk for local and systemic infections. More than 900,000 episodes of catheter-associated urinary tract infections occur annually in acute-care hospitals in the United States, accounting for 40% of all nosocomial infections and involving between 10 and 30% of patients with indwelling urinary catheters (30). Catheter-associated urinary tract infection prolongs the hospital stay between an estimated 2.4 and 4.5 days, with resultant increased healthcare costs (15,16). Recent studies have shown that a wide range of persistent catheter-related infections may be related to the ability of bacteria to form biofilms (6, 28). Treatment of device-related infections with conventional antimicrobial agents frequently fails because microorganisms growing in biofilms are more tolerant or phenotypically resistant to antimicrobial agents than planktonic cells (24). The insensitivity of biofilm bacteria to antibiotics is a function of cell wall composition, surface structure, and phenotypic variation in enzymatic activity (8,14). It has also been suggested that the negatively charged exopolysaccharide is very effective in protecting bacterial cells from cationic antibiotics by restricting their permeation (2...

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mentioning

confidence: 99%

Antibiofilm Activity of GlmU Enzyme Inhibitors against Catheter-Associated Uropathogens

Burton

Gawande

Yakandawala

et al. 2006

Antimicrob Agents Chemother

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“…The mechanism of antibacterial activity proposed is associated with an initial inhibition of primary adherence due to the hydrogel hydrophilicity, followed by secondary biocidal activity of the silver coating [32,34].…”

Section: Discussionmentioning

confidence: 99%

The potential of photo-deposited silver coatings on Foley catheters to prevent urinary tract infections

Cooper

Pollini

Paladini

2016

Materials Science and Engineering: C

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Catheter-associated urinary tract infection (CAUTI) represents one of the most common causes of morbidity and mortality. The resistance demonstrated by many microorganisms to conventional antibiotic therapies and the in-creasing health-care costs have recently encouraged the definition of alternative preventive strategies, which can have a positive effect in the management of infections.Antimicrobial urinary catheters have been developed through the photo-chemical deposition of silver coatings on the external and luminal surfaces. The substrates are exposed to ultraviolet radiation after impregnation into a silver-based solution, thus inducing the in situ synthesis of silver particles. The effect of the surface treatment on the material was investigated through scanning electron microscopy (SEM) and silver ion release measurements. The ability of microorganisms commonly associated with urinary tract infections was investigated in terms of bacterial viability, proliferation and biofilm development, using Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis as target organisms. The silver coatings demonstrated good distribution of silver particles to the substrate, and proved an effective antibacterial capability in simulated biological conditions.The low values of silver ion release demonstrated the optimum adhesion of the coating. The results indicated a good potential of silver-based antimicrobial materials for prevention of catheterassociated urinary tract infection.

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“…Bu enfeksiyonların önüne geçmek amacıyla son yıllarda bazı antimikrobiyal ajanlar ve biyofilm oluşumunu inhibe edici maddelerle kaplanmış kateterler kullanıma girmiştir (23)(24)(25) . Gümüş kaplı kateter kullanıldığında üriner infeksiyon gelişme oranı %30'luk bir azalma göstermiştir (26) .…”

Section: Discussionunclassified

Investigation of Biofilm Formation of ESBL Positive Uropathogenic Escherichia coli Isolates on (Microplate) Surfaces Modified with Plasma Polymerization Technique and Nanomaterials: An Experimental Model

Hortaç¹,

Kaleli²,

Çökeliler³

et al. 2016

TMCD

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Effects of Hydrogel/Silver Coatings on In Vitro Adhesion to Catheters of Bacteria Associated with Urinary Tract Infections

Cited by 127 publications

References 6 publications

Antibiofilm Activity of GlmU Enzyme Inhibitors against Catheter-Associated Uropathogens

Antibiofilm Activity of GlmU Enzyme Inhibitors against Catheter-Associated Uropathogens

The potential of photo-deposited silver coatings on Foley catheters to prevent urinary tract infections

Investigation of Biofilm Formation of ESBL Positive Uropathogenic Escherichia coli Isolates on (Microplate) Surfaces Modified with Plasma Polymerization Technique and Nanomaterials: An Experimental Model

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