Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation

Qin, Jing; Hua, Yi-Ming

doi:10.3892/mmr.2016.5680

Cited by 13 publications

(9 citation statements)

References 47 publications

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“…Osteoblasts, the main cells in bone tissue and bone formation, are critical for alveolar bone morphology and the stability of the moved tooth (Aksu et al 2010 ). Continuous exposure to mechanical force during orthodontic treatment promotes the function of mature osteoblasts, as well as increasing bone formation and differentiation of osteoblast precursors (Fu et al 2016 ; Dai et al 2017 ; Qin and Hua 2016 ), which is in line with the results of the current study. It’s believed that bone formation is significantly increased at tension sites during orthodontic treatment, which is partially responsible for the effect of OTM.…”

Section: Discussionsupporting

confidence: 91%

Tension force-induced bone formation in orthodontic tooth movement via modulation of the GSK-3β/β-catenin signaling pathway

et al. 2017

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Orthodontic force-induced osteogenic differentiation and bone formation at tension sites play a critical role in orthodontic tooth movement. However, the molecular mechanism underlying this phenomenon is poorly understood. In the current study, we investigated the involvement of the GSK-3β/β-catenin signaling pathway, which is critical for bone formation during tooth movement. We established a rat tooth movement model to test the hypothesis that orthodontic force may stimulate bone formation at the tension site of the moved tooth and promote the rate of tooth movement via regulation of the GSK-3β/β-catenin signaling pathway. Our results showed that continued mechanical loading increased the distance between the first and second molar in rats. In addition, the loading force increased bone formation at the tension site, and also increased phospho-Ser9-GSK-3β expression and β-catenin signaling pathway activity. Downregulation of GSK-3β activity further increased bone parameters, including bone mineral density, bone volume to tissue volume and trabecular thickness, as well as ALP- and osterix-positive cells at tension sites during tooth movement. However, ICG-001, the β-catenin selective inhibitor, reversed the positive effects of GSK-3β inhibition. In addition, pharmaceutical inhibition of GSK-3β or local treatment with β-catenin inhibitor did not influence the rate of tooth movement. Based on these results, we concluded that GSK-3β/β-catenin signaling contributes to the bone remodeling induced by orthodontic forces, and can be used as a potential therapeutic target in clinical dentistry.

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Section: Discussionsupporting

confidence: 91%

Tension force-induced bone formation in orthodontic tooth movement via modulation of the GSK-3β/β-catenin signaling pathway

et al. 2017

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“…The results of the present study revealed that H 2 S could up-regulate the process of osteogenesis, osteoclastic activities and bone remodeling. These findings are consistent with our previous study, where mechanical stimulation and H 2 S could enhance the osteogenic differentiation ( 18 , 19 , 35 ). These results were also in accordance with some studies illustrating that H 2 S could enhance RANKL-induced osteoclast differentiation in vitro and induce osteoclast differentiation in vivo ( 17 , 36 ).…”

Section: Discussionsupporting

confidence: 94%

“…Our previous in vitro study also demonstrated that H 2 S could promote osteogenic differentiation by up-regulating the expression ratio of OPG/RANKL expression in human PDLSCs ( 18 ). It was also demonstrated that H 2 S significantly increased the expression of ALP, OCN, runt-related transcription factor 2 and collagen 1 in the human PDLSCs with tension force stimulation, and promoted osteogenic differentiation of human PDLSCs by activating the p38-MAPK and ERK signaling pathways ( 19 , 35 ). In the present study, exogenous H 2 S could up-regulate the expression of ALP and OCN, the ratio of RANKL/OPG and the number of osteoclasts in the alveolar bone.…”

Section: Discussionmentioning

confidence: 97%

Hydrogen sulfide regulates bone remodeling and promotes orthodontic tooth movement

Hua

2017

Molecular Medicine Reports

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Hydrogen sulfide (H2S) is a gas signaling molecule that has multiple influences on physiological and pathological processes in the mammalian body, including vasodilation, neurotransmission, inflammation, hypoxia sensing and bone remodeling. Our previous studies suggested that H2S might be involved in the periodontal tissue remodeling during the orthodontic tooth movement (OTM) via increasing periodontal ligament cell differentiation, tissue mineralization, bone formation and collagen synthesis. The aim of the present study was to investigate the effects of H2S on alveolar bone remodeling that is associated with tooth movement. Experiments were performed in an OTM mouse model. Sodium hydrosulfide (NaHS), which is a donor of H2S and DL-propargylglycine (PAG) and a cystathionine-γ-lyase (CSE) inhibitor, which could also decrease H2S expression, were administered intraperitoneally and respectively. A total of 60 male C57BL6/J mice were divided into 4 groups; Control, NaHS, PAG and combination (PAG+NaHS). The rate of OTM and the bone mineral density (BMD) of alveolar bone were scanned and measured by micro-computed tomography (micro-CT). The number of osteoclasts and expression of the tumor necrosis factor ligand superfamily member-11 (RANKL), alkaline phosphatase (ALP), osteocalcin (OCN) and osteoprotegerin (OPG) in alveolar bone were accessed to evaluate the osteoclastic activity and osteogenesis with histochemistry of tartrate-resistant acid phosphatase staining, immunohistochemistry and reverse transcription-quantitative polymerase chain reaction. In the alveolar bone, NaHS increased the OTM and decreased the BMD, respectively. PAG significantly decrease OTM and increased the BMD. NaHS combined with PAG rescued the PAG-induced changes in the OTM and the BMD. Additionally, the number of osteoclasts, the expression of RANKL, ALP, OCN and the ratio of RANKL/OPG were significantly up-regulated in the NaHS group. In contrast, PAG down-regulated the number of osteoclasts, the expression of RANKL, ALP, OCN and the ratio of RANKL/OPG. These findings suggested that H2S might facilitate the OTM by enhancing alveolar bone remodeling as a result of an increased osteoclastic activity and osteogenesis.

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“…In contrast, one study reported a slight decrease in BGLAP gene expression and it was assumed that an enhanced cell proliferation of young osteoblasts might be responsible (Jacobs et al, 2013). Another study described a transient upregulation of BGLAP following 3 h of exposure against tension force (Qin and Hua, 2016).…”

Section: Osteogenesismentioning

confidence: 98%

Effect of Tension on Human Periodontal Ligament Cells: Systematic Review and Network Analysis

Sun

Ranković

Folwaczny

et al. 2021

Front. Bioeng. Biotechnol.

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Orthodontic tooth movement is based on the remodeling of tooth-surrounding tissues in response to mechanical stimuli. During this process, human periodontal ligament cells (hPDLCs) play a central role in mechanosensing and mechanotransduction. Various in vitro models have been introduced to investigate the effect of tension on hPDLCs. They provide a valuable body of knowledge on how tension influences relevant genes, proteins, and metabolites. However, no systematic review summarizing these findings has been conducted so far. Aim of this systematic review was to identify all related in vitro studies reporting tension application on hPDLCs and summarize their findings regarding force parameters, including magnitude, frequency and duration. Expression data of genes, proteins, and metabolites was extracted and summarized. Studies’ risk of bias was assessed using tailored risk of bias tools. Signaling pathways were identified by protein-protein interaction (PPI) networks using STRING and GeneAnalytics. According to our results, Flexcell Strain Unit® and other silicone-plate or elastic membrane-based apparatuses were mainly adopted. Frequencies of 0.1 and 0.5 Hz were predominantly applied for dynamic equibiaxial and uniaxial tension, respectively. Magnitudes of 10 and 12% were mostly employed for dynamic tension and 2.5% for static tension. The 10 most commonly investigated genes, proteins and metabolites identified, were mainly involved in osteogenesis, osteoclastogenesis or inflammation. Gene-set enrichment analysis and PPI networks gave deeper insight into the involved signaling pathways. This review represents a brief summary of the massive body of knowledge in this field, and will also provide suggestions for future researches on this topic.

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Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation

Cited by 13 publications

References 47 publications

Tension force-induced bone formation in orthodontic tooth movement via modulation of the GSK-3β/β-catenin signaling pathway

Tension force-induced bone formation in orthodontic tooth movement via modulation of the GSK-3β/β-catenin signaling pathway

Hydrogen sulfide regulates bone remodeling and promotes orthodontic tooth movement

Effect of Tension on Human Periodontal Ligament Cells: Systematic Review and Network Analysis

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