Effects of hydroxybenzyl alcohols on melanogenesis in melanocyte-keratinocyte co-culture and monolayer culture of melanocytes

Liu, Szu-Hsiu; Chu, I‐Ming; Pan, I-Horng

doi:10.1080/14756360701654894

Cited by 11 publications

(8 citation statements)

References 31 publications

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“…Consistent with the in vitro study, 1.25 mg/cm 2 and 2.5 mg/cm 2 LSE treatments suppressed melanogenesis by inhibiting PKA and p38 activation. Earlier studies have shown that the melanin production in melanocytes is influenced by keratinocytes and fibroblasts [30,31]. Primary melanocytes and keratinocytes have some drawbacks such as the limited proliferation capacity and the donor variability [31].…”

Section: Discussionmentioning

confidence: 99%

“…Earlier studies have shown that the melanin production in melanocytes is influenced by keratinocytes and fibroblasts [30,31]. Primary melanocytes and keratinocytes have some drawbacks such as the limited proliferation capacity and the donor variability [31]. Primary murine melanocytes defective at the Mc1r locus have been historically difficult to culture in vitro [32].…”

Section: Discussionmentioning

confidence: 99%

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Anti-Melanogenesis Effects of Lotus Seedpod In Vitro and In Vivo

Hsu

Lin

et al. 2020

Nutrients

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Melanogenesis has many important physiological functions. However, abnormal melanin production causes various pigmentation disorders. Melanin synthesis is stimulated by α-melanocyte stimulating hormone (α-MSH) and ultraviolet (UV) irradiation. Lotus seedpod extract (LSE) has been reported as possessing antioxidative, anti-aging, and anticancer activities. The present study examined the effect of LSE on melanogenesis and the involved signaling pathways in vitro and in vivo. Results showed that non-cytotoxic doses of LSE and its main component epigallocatechin (EGC) reduced both tyrosinase activity and melanin production in the α-MSH-induced melanoma cells. Western blotting data revealed that LSE and EGC inhibited expressions of tyrosinase and tyrosinase-related protein 1 (TRP-1). Phosphorylation of p38 and protein kinase A (PKA) stimulated by α-MSH was efficiently blocked by LSE treatment. Furthermore, LSE suppressed the nuclear level of cAMP-response element binding protein (CREB) and disturbed the activation of melanocyte inducing transcription factor (MITF) in the α-MSH-stimulated B16F0 cells. The in vivo study revealed that LSE inhibited melanin production in the ear skin of C57BL/6 mice after exposure to UVB. These findings suggested that the anti-melanogenesis of LSE involved both PKA and p38 signaling pathways. LSE is a potent novo natural depigmenting agent for cosmetics or pharmaceutical applications.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anti-Melanogenesis Effects of Lotus Seedpod In Vitro and In Vivo

Hsu

Lin

et al. 2020

Nutrients

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“…0.5 × 10 4 melanocytes : 5 × 10 4 keratinocytes) in the mixed medium and incubated at 37°C, 5% CO 2 . The initial seeding ratio of melanocytes to keratinocytes used a 1:10 ratio according to the previous study 5 . We mixed the culture media with various concentration ratios (1:1, 1:2, 1:5 and 1:10) of advanced iDMM to keratinocyte growth medium, respectively.…”

Section: Figurementioning

confidence: 99%

Establishment of co‐culture of human induced pluripotent stem cell‐derived melanocytes and keratinocytes in vitro

et al. 2020

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Dear Editor,We previously established human induced pluripotent stem (iPS) cell-derived melanocytes in large quantities within a short period and induced their differentiation and high melanogenic potency using original iPS cell-derived melanocyte medium (iDMM). 1 We used original iPS cell lines established from T cells in the blood of a healthy 40-year-old Japanese man and a Sendai virus vector. 1 In the present study, we established melanocytes derived from more general iPS cells (1383D6, HPS1006) obtained from the Japanese Center for iPS Cell Research and Application using advanced iDMM (Table S1). The iPS cells were maintained in StemFit medium (AHS, AK02N; Ajinomoto, Tokyo, Japan). We found that many pigmented cells developed from the border of human iPS cell nests within 10 days under advanced iDMM (Fig. 1a). The granules in them were strongly tyrosinase-positive as observed by confocal immunofluorescence laser microscopy, which suggested that they were mature melanocytes (Fig. 1b). Previous approaches to grow and differentiate iPS cells into melanocytes needed first to produce embryoid bodies and then to process them to differentiate into melanocytes, which took approximately 30 days. 2,3 The present approach further improves in production of large quantities within a short period and induces differentiation and high melanogenic potency in vitro. We examined the mRNA expression levels of NANOG, Pax3, MITF, TRP1 and tyrosinase in the human iPS cell-derived melanocytes using quantitative reverse transcription polymerase chain reaction (Table S2, Fig. 1c). Normal human adult epidermal melanocytes were purchased from Invitrogen Gibco (Carlsbad, CA, USA) as controls (Table S3).The mRNA expression level of the pluripotency marker NANOG in these iPS cell-derived melanocytes was significantly lower than in human iPS cells. In contrast, mRNA expression levels of four melanocyte-specific markers (Pax3, MITF, TRP1 and tyrosinase) in the iPS cell-derived melanocytes that we established from a line of human melanocytes were significantly higher than in human iPS cells. The melanocyte-specific marker mRNA expression levels in the iPS cell-derived melanocytes were similar to the levels found in normal human adult epidermal melanocytes (Table S4).

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“…Monolayer models are useful for studying melanocyte properties and testing different conditions or drugs. In 2008, in a comparative study of melanocytes culture and melanocytes-keratinocytes co-culture, Liu et al demonstrated the effect of melanogenic stimulators (α-MSH and L-tyrosine) and inhibitors (arbutin and hydroxybenzyl alcohols (HBA)) in the two conditions [113]. Results showed that α-MSH and L-tyrosine increased the melanin content of melanocytes, and that the increase was better in the co-culture with keratinocytes.…”

Section: Monolayersmentioning

confidence: 99%