Effects of hydroxypropyl-β-cyclodextrin on steroids 1-en-dehydrogenation biotransformation by Arthrobacter simplex TCCC 11037

Preparation, Characterization, and Biotransformation of the Inclusion Complex of Phytosterols and Hydroxypropyl-β- cyclodextrin by Mycobacterium neoaurum

“…This shows that complexation with HBβCD substantially enhanced the conversion of PSs by Brought to you by | Mount Royal University Authenticated Download Date | 6/14/15 10:00 PM M. neoaurum in an aqueous fermentation system to a mixture of AD and ADD. The optimal ratio of HBβCD and the PSs was 2:1, which increased the specifi c side-chain cleavage activity with some negative infl uence on cell growth (Wang et al, 2009). When the ratio of HBβCD and PSs was 2:1, the AD(D) yield was 72.2% compared to the control after 10 min of ultrasonic treatment of the PSs-HBβCD complexe.…”

“…PSs have a low solubility in water, but after complexation the solubility of PSs increases signifi cantly (Hesselink et al, 1989;Manosroi et al, 2008;Wang et al, 2009). The effects of ratio and ultrasonication time of the PSs-HBβCD complexe on cell growth and AD(D) production are shown in Fig.…”

“…Some studies have reported that complexation of steroids with certain types of β-cylodextrins (β-CD), using the inclusion technique, could result in increased solubility of steroids and a positive effect on the production rate (Calderini and Pessine, 2008;Manosroi et al, 2005Manosroi et al, , 2008Wang et al, 2009), but only few focused on the thermal stability of the complexes. The aims of the present investigation were to study the effects of complexation on thermal stability, solubility, and biotransformation capability of PSs and HBβCD complexes.…”

The influence of host–guest inclusion complex formation on the biotransformation of cortisone acetate Δ1-dehydrogenation

“…We had used HP-␤-CD in order to enhance cortisone acetate (CA) bioavailability in aqueous transformation media and consequently to increase the rate of steroid 1 -dehydrogenation. This work not only provided a systematic study on the influences of HP-␤-CD on the CA 1 -dehydrogenation reaction, but also suggested an applicable and effective method for enhancing enzyme-catalyzed steroids biotransformation [22].…”

Improvement of the solubility and bioavailability of loratadine by pharmaceutical technological mtehods

“…CD complexation represents a unique and effective strategy for improving the protein therapy by stabilizing them against aggregation, thermal denaturation and degradation [82]. It not only increases the reaction rate but also improves the final substrate conversion rate [83].…”