Effects of hydroxytyrosol dose on the redox status of exercised rats: the role of hydroxytyrosol in exercise performance

Fazazi, Saad Al; Casuso, Rafael A.; Aragón‐Vela, Jerónimo; Casals, Cristina; Huertas, Jesús R.

doi:10.1186/s12970-018-0221-3

Cited by 18 publications

(20 citation statements)

References 41 publications

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“…In this regard, the main limitation of the present study is that we have not quantified SKM oxidative status. However, the redox reactions produced in living cells following polyphenol Boots et al, 2007). This pro-oxidant effect has been reported in exercised rats supplemented with 12 mg•kg•d of quercetin (Casuso et al, 2015).…”

Section: Discussionmentioning

confidence: 93%

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Hydroxytyrosol modifies skeletal muscle GLUT4/AKT/Rac1 axis in trained rats

Casuso

Fazazi

Ruiz‐Ojeda

et al. 2020

Journal Cellular Physiology

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Training induces a number of healthy effects including a rise in skeletal muscle (SKM) glucose uptake. These adaptations are at least in part due to the reactive oxygen species produced within SKM, which is in agreement with the notion that antioxidant supplementation blunts some training-induced adaptations. Here, we tested whether hydroxytyrosol (HT), the main polyphenol of olive oil, would modify the molecular regulators of glucose uptake when HT is supplemented during exercise. Rats were included into sedentary and exercised (EXE) groups. EXE group was further divided into a group consuming a low HT dose (0.31 mg•kg•d; EXElow), a moderate HT dose (4.61 mg•kg•d; EXEmid), and a control group (EXE). EXE raised glucose transporter type 4 (GLUT4) protein content, Ras-related C3 botulinum toxin substrate 1 (Rac1) activity, and protein kinase b (AKT) phosphorylation in SKM. Furthermore, EXElow blunted GLUT4 protein content and AKT phosphorylation while EXEmid showed a downregulation of the GLUT4/AKT/Rac1 axis. Hence, a low-to-moderate dose of HT, when it is supplemented as an isolated compound, might alter the beneficial effect of training on basal AKT phosphorylation and Rac1 activity in rats.

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Section: Discussionmentioning

confidence: 93%

“…Deleterious effects on exercise performance have been reported in rodents consuming 20 mg·kg·d (Al Fazazi et al, 2018), a dose with high antioxidant potential within SKM (Cao et al, 2014). Notably, here we used significant lower HT doses that are known to be able to rise plasma antioxidant capacity.…”

Section: Discussionmentioning

confidence: 99%

Hydroxytyrosol modifies skeletal muscle GLUT4/AKT/Rac1 axis in trained rats

Casuso

Fazazi

Ruiz‐Ojeda

et al. 2020

Journal Cellular Physiology

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“…In this way, previous studies have demonstrated the cardioprotective effect of VOO enriched in bioactive compounds from the olive oil in animals [8] and humans [9,10]. In fact, recent studies have shown a strong ergogenic effect on physical activity and remodeling of more efficient mitochondrial super-complexes with lower reactive oxygen-derived species production at doses 20 mg/kg and 300 mg/kg [11,12].…”

Section: Introductionmentioning

confidence: 93%

Acute/Subacute and Sub-Chronic Oral Toxicity of a Hidroxytyrosol-Rich Virgin Olive Oil Extract

et al. 2019

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The objective of this study was to determine the acute (one single dose), subacute (14 days), and sub-chronic (90 days) toxicity of an aqueous virgin olive oil (VOO) extract rich in hydroxytyrosol in rats. For acute/subacute toxicity, rats were divided into three groups. The control group received distilled water (n = 9), another experimental group received a single dose of 300 mg/kg (n = 3), and a third group received one dose of 2000 mg/kg (n = 4) during 14 days. The sub-chronic study included 60rats distributed in three groups (n = 20: 10 males and 10 females) receiving daily different three doses of the VOO extract in the drinking water during 90 days: (1) 100 mg/kg, (2) 300 mg/kg, and (3) 1000 mg/kg. In parallel, a fourth additional group (n = 20: 10 males and 10 females) did not receive any extract (control group). Clinical signs, body weight, functional observations of sensory and motor reactivity, hematological and biochemical analyses, and macroscopic and microscopic histopathology were evaluated. No adverse effects were observed after the administration of the different doses of the hydroxytyrosol-rich VOO extract, which suggests that the enrichment of VOO in its phenolic compound is safe, and can be used as functional foods for the treatment of chronic degenerative diseases.

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“…17,20 LPS intoxicated group (septic model): mice in this group were injected intraperitoneally with a single dose of LPS (10 mg/ kg) to induce septic response according to a previous report. 21 HT 20 mg+LPS: mice in this group were gavaged with HT 20 mg/kg for 10 consecutive days according to an earlier studies, 20,22 then injected intraperitoneally with a single dose of LPS (10 mg/ kg). HT 40 mg+ LPS: mice in this group were gavaged with HT 40 mg/kg for 10 consecutive days, then injected intraperitoneally with a single dose of LPS (10 mg/ kg). …”

Section: Methodsmentioning

confidence: 99%

“…HT 20 mg+LPS: mice in this group were gavaged with HT 20 mg/kg for 10 consecutive days according to an earlier studies, 20,22 then injected intraperitoneally with a single dose of LPS (10 mg/ kg).…”

Section: Methodsmentioning

confidence: 99%

Hydroxytyrosol ameliorates oxidative challenge and inflammatory response associated with lipopolysaccharide-mediated sepsis in mice

Alblihed

2020

Hum Exp Toxicol

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Hydroxytyrosol (HT) is among the main bioactive ingredients isolated from olive tree with a variety of biological and pharmacological activities. In the current study, the antioxidative and anti-inflammatory activities of HT were distinguished in the splenic tissue following lipopolysaccharide (LPS)-mediated septic response. Thirty-five Swiss mice were divided into five groups (n = 7): control, HT (40 mg/kg), LPS (10 mg/kg), HT 20 mg+LPS and HT 40 mg+LPS. HT was administered for 10 days, while a single LPS dose was applied. The obtained findings demonstrate that HT administration enhanced the survival rate and decreased lactate dehydrogenase level in LPS-challenged mice. Treatment with HT inhibited the incidence of oxidative damage in splenic tissue through decreasing lipoperoxidation and increasing antioxidant molecules, namely glutathione, superoxide dismutase and catalase. HT also decreased total leukocytes count, C-reactive protein, monocyte chemoattractant protein-1, and myeloperoxidase levels. Additionally, HT suppressed the production levels of tumor necrosis factor-α, interleukin-1β, and interleukin-6. Moreover, mRNA expression of inducible nitric oxide synthase and nitric oxide production were increased after HT administration. Furthermore, HT supplementation resulted in a downregulation of p38 mitogen-activated protein kinase, inhibited the activation of the nuclear factor kappa-B from the nucleus to the cytoplasm, and attenuated infiltration of activated immune cells and tissue injury following LPS injection. Collectively, these findings demonstrate the antioxidative and anti-inflammatory properties of HT against LPS-mediated inflammation and sepsis. Therefore, HT could be applied as an alternative anti-inflammatory agent to minimize or prevent the development of systemic inflammatory response associated with septic shock.

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Effects of hydroxytyrosol dose on the redox status of exercised rats: the role of hydroxytyrosol in exercise performance

Cited by 18 publications

References 41 publications

Hydroxytyrosol modifies skeletal muscle GLUT4/AKT/Rac1 axis in trained rats

Hydroxytyrosol modifies skeletal muscle GLUT4/AKT/Rac1 axis in trained rats

Acute/Subacute and Sub-Chronic Oral Toxicity of a Hidroxytyrosol-Rich Virgin Olive Oil Extract

Hydroxytyrosol ameliorates oxidative challenge and inflammatory response associated with lipopolysaccharide-mediated sepsis in mice

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