Effects of hyperthermia on the cytoskeleton and focal adhesion proteins in a human thyroid carcinoma cell line

Huang, Shifeng; Yang, Kun; Wu, Jyh‐Lin; Chang, Kai-Chi; Wang, Seu Mei

doi:10.1002/(sici)1097-4644(19991101)75:2<327::aid-jcb14>3.0.co;2-g

Cited by 26 publications

(13 citation statements)

References 28 publications

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“…The cytoskeleton is a cellular component that is common to the plasma membrane, nuclear membrane, and cytoplasmic organelles. The three components of the cytoskeleton (i.e., actin filaments, microtubules, and intermediate filaments) work together to enhance structural integrity, impart cell shape, provide cell motility, and position mRNA and protein [31,34], and they play an important role in cell signaling [37], mitosis, and cytokinesis [38]. During the first three cleavage divisions, the mammalian embryo spends the majority of the cell cycle in S and M phases [39].…”

Section: Discussionmentioning

confidence: 99%

Alterations in Ultrastructural Morphology of Two-Cell Bovine Embryos Produced In Vitro and In Vivo Following a Physiologically Relevant Heat Shock1

Rivera

Kelley

Erdos

et al. 2003

Biology of Reproduction

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Exposure of cultured preimplantation embryos to temperatures similar to those experienced by heat-stressed cows inhibits subsequent development. In this study, the effects of heat shock on the ultrastructure of two-cell bovine embryos were examined to determine mechanisms for inhibition of development. Two-cell embryos produced in vitro were harvested at approximately 28 h postinsemination and cultured for 6 h at one of three temperatures: 38.5 degrees C (cow body temperature), 41.0 degrees C (characteristic temperature for heat-stressed cows), or 43.0 degrees C (severe heat shock). Ultrastructural examinations revealed that both heat shocks resulted in the movement of organelles towards the center of the blastomere. In addition, heat shock increased the percentage of mitochondria exhibiting a swollen morphology. Distance between the membranes comprising the nuclear envelope was increased but only when embryos were treated at 43.0 degrees C. To determine whether ultrastructural responses to heat shock in culture were similar for embryos produced in vitro and in vivo, two-cell embryos were collected from superovulated Angus cows 48 h postinsemination and treated ex vivo for 6 h at 38.5 degrees C or 41.0 degrees C. Again, heat shock caused an increase in number of swollen mitochondria and movement of organelles away from the periphery of the blastomere. Exposure of two-cell bovine embryos to physiologically relevant elevated temperatures causes disruption in ultrastructural morphology that is inimical to development. The observation that overall morphology and response to heat was similar for embryos produced in vitro and in vivo implies that the former can be a good model for understanding embryonic responses to heat shock.

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Section: Discussionmentioning

confidence: 99%

Alterations in Ultrastructural Morphology of Two-Cell Bovine Embryos Produced In Vitro and In Vivo Following a Physiologically Relevant Heat Shock1

Rivera

Kelley

Erdos

et al. 2003

Biology of Reproduction

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“…Sometimes the terms mild hyperthermia or just hyperthermia is used for temperatures up to 42 °C and extreme hyperthermia for higher temperatures [ 23 ]. At those temperatures, heat alone may cause protein denaturation [ 24 ], damage to the cytoskeleton [ 25 , 26 ], impairment of certain DNA repair processes [ 27 , 28 , 29 , 30 , 31 , 32 ], changes in cell membrane permeability [ 33 , 34 ], and stimulation of the immune system [ 35 , 36 , 37 , 38 ].…”

Section: Hyperthermiamentioning

confidence: 99%

Magnetic Hyperthermia and Radiation Therapy: Radiobiological Principles and Current Practice †

et al. 2018

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Hyperthermia, though by itself generally non-curative for cancer, can significantly increase the efficacy of radiation therapy, as demonstrated by in vitro, in vivo, and clinical results. Its limited use in the clinic is mainly due to various practical implementation difficulties, the most important being how to adequately heat the tumor, especially deep-seated ones. In this work, we first review the effects of hyperthermia on tissue, the limitations of radiation therapy and the radiobiological rationale for combining the two treatment modalities. Subsequently, we review the theory and evidence for magnetic hyperthermia that is based on magnetic nanoparticles, its advantages compared with other methods of hyperthermia, and how it can be used to overcome the problems associated with traditional techniques of hyperthermia.

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“…HS has many effects on a cell, including detachment of adherent cells from their substrate (30). We used HS as a tool to analyze the effect of Hst1 on cell attachment and spreading in the presence of serum.…”

Section: Hst1 Promotes Cell Spreading After Hsmentioning

confidence: 99%

Histatin-1, a histidine-rich peptide in human saliva, promotes cell-substrate and cell-cell adhesion

Dijk¹,

Nazmi²,

Bolscher³

et al. 2015

The FASEB Journal

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Histatins (Hsts) are histidine-rich peptides exclusively present in the saliva of higher primates. In this study, we explored the effects of Hsts on cell-substrate and cell-cell adhesion. Histatin (Hst)-1 caused a significant (>2-fold) increase (EC 50 = 1 mM) in the ability of human adherent cells to attach and spread, even in conditions that impaired cell spreading. Other tested Hsts did not stimulate cell spreading, indicating a specific effect of Hst1. The effect of Hst1 on cell-cell adhesion was investigated by using transepithelial resistance (TER) measurements in the human cell line Caco-2, a widely used model for the epithelial layer. We found that 10 mM Hst1 caused a 20% increase in TER compared to the negative control, indicating a function for Hst1 in intercellular cell adhesion and epithelial integrity. A role for Hst1 in both cellsubstrate and cell-cell adhesion is highly conceivable, because these 2 modes of adhesion are closely related via shared components and connected signaling pathways.-Van Dijk, I. A., Nazmi, K., Bolscher, J. G. M., Veerman, E. C. I., Stap, J. Histatin-1, a histidine-rich peptide in human saliva, promotes cell-substrate and cell-cell adhesion. FASEB J. 29, 3124-3132 (2015). www.fasebj.org

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Effects of hyperthermia on the cytoskeleton and focal adhesion proteins in a human thyroid carcinoma cell line

Cited by 26 publications

References 28 publications

Alterations in Ultrastructural Morphology of Two-Cell Bovine Embryos Produced In Vitro and In Vivo Following a Physiologically Relevant Heat Shock1

Alterations in Ultrastructural Morphology of Two-Cell Bovine Embryos Produced In Vitro and In Vivo Following a Physiologically Relevant Heat Shock1

Magnetic Hyperthermia and Radiation Therapy: Radiobiological Principles and Current Practice †

Histatin-1, a histidine-rich peptide in human saliva, promotes cell-substrate and cell-cell adhesion

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