Effects of hypoxia on the mRNA expressions of TRAIL-mediated cell death related genes in hypoxia-tolerant rodent (Nannospalax nehringi) and some characteristics of these proteins

Abstract

The aim of the present study was to determine the mRNA expression profiles of TRAILmediated cell death related genes of the hypoxia-tolerant rodent Nannospalax nehringi under hypoxia. The nucleotide and amino acid sequences of these genes were identified. Captured Nannospalax nehringi were randomly divided into normoxia and hypoxia groups. The hypoxia group was exposed to a 7% O2 + 93% N2 gas mixture for 52 h, while animals in the normoxia group were housed under normoxic conditions. Total RNAs were isolated from brain and lung tissues. The cIAP-1, cIAP-2, XIAP, DcR1, DcR2, FLIP and OPG genes were PCR amplified, and the cIAP-1, cIAP-2, XIAP, OPG, TRAIL and FLIP genes were sequenced. Sequenced genes were translated into amino acid sequences and compared with reliable sequences of closely similar species. The genes in the brain were regulated for protection against hypoxia; however, the genes in the lung were regulated differently. Many mutations and insertions were observed on the conserved regions of the cIAP-1, cIAP-2, XIAP, OPG and TRAIL genes in N. nehringi. We propose that these gene mutations and insertions contribute to the anti-hypoxic properties of N. nehringi.

Effect of Fasciola Hepatica Invasion on Cow Productivity

“…At the same time, despite all the efforts made by science and practice, the Fasciola hepatica parasite is still common and very common in cattle. The situation is complicated by the fact that, as a rule, fascioliasis in cattle is mostly asymptomatic (Zavalishina, 2018d;Yıldız et al, 2021), but it causes serious physiological changes that lead to significant economic losses (Torgerson & Claxton, 1999). Despite this fact, few studies related to the impact of the disease on productivity levels have been performed on this parasitic disease (Vercruysse & Claerebout, 2001;Drljača et al, 2020).…”