Effects of Lactobacillus Casei YBJ02 on Lipid Metabolism in Hyperlipidemic Mice

Abstract

Traditionally fermented yak yogurt as a Tibetan dairy product is high not only in nutrients but also in probiotics. A probiotic strain with a potential lipid reducing effect was isolated from yak yogurt. An animal model for hyperlipidemia was evaluated using the blood index and expression levels of lipid metabolism-related proteins in mice to determine the effect of Lactobacillus casei YBJ02 (LC-YBJ02) on lipid metabolism in hyperlipidemic mice and the underlying mechanism. LC-YBJ02 at different concentrations exhibited certain inhibitory effects on the increase in blood lipid in mice. Particularly, high concentrations of LC-YBJ02 can reduce the cholesterol, triglycerides, and low-density lipoprotein content; however, no significant effect on the high-density lipoprotein of the body has been reported. LC-YBJ02 can effectively increase the reduction in cholesterol level by fecal excretion. In this study, the gene-affecting mouse obesity was determined using experimental results in mice. The expression levels of peroxisome proliferator-activated receptor γ (PPARγ ), CCAAT enhancer binding protein alpha (CEBP)/α, sterol regulatory element binding protein (SREBP)-1c, and FAS could improve because of the high-fat diet in kidney fat. Bacteria at different concentrations could also decrease these expression levels. Specifically, the high concentration of LC-YBJ02 could suppress the expression of PPARγ , CEBP/α, and SREBP-1c; however, the expression of FAS was not significantly inhibited. PPARγ and FAS expression levels in the liver were low, but no significant difference was indicated. CEBP/α and SREBP-1c expression in mouse liver was further detected by Western blot analysis; CEBP/α was considerably low and could not be detected. On the basis of these results, LC-YBJ02 could be used as probiotics through its lipid reduction effects.

Feeding of cuticles from Tenebrio molitor larvae modulates the gut microbiota and attenuates hepatic steatosis in obese Zucker rats

“…Like Bifidobacteriaceae, several probiotic species of the Lactobacillaceae family have been shown to either prevent or alleviate hepatic lipid accumulation in rodent models of fatty liver disease, obesity or metabolic syndrome. 54,55 Further characteristic changes in the gut microbiota composition at the family level in group O3.0 were increases of Erysipelotrichaceae (Firmicutes phylum), Prevotellaceae (Bacteroidetes phylum) and Sutterellaceae (Proteobacteria phylum) and decreases of Anaeroplasmataceae (Tenericutes phylum), Desulfovibrionaceae (Proteobacteria phylum), Eubacteriaceae, Ruminococcaceae (both Firmicutes phylum) and Saccharibacteria (Candidatus saccharibacteria phylum). Despite the biological meaning of all of these gut microbiota modifications induced by cuticle supplementation is unclear, similar changes in several of these bacterial populations (increases of Prevotellaceae, Bifidobacteriaceae and Coriobacteriaceae, decrease of Desulfovibrionaceae) are in line with those found in other obese rodent models, in which antisteatotic and hepatoprotective effects have been observed.…”

Hypoglycemic effects of space‐induced Lactobacillus plantarum SS18‐5 on type 2 diabetes in a rat model

“…Many studies have shown that continuous intake of high‐glucose‐fat diets can lead to abnormal lipid metabolism in T2DM rats, especially an increase in circulating FFAs, which is a typical characteristic of insulin resistance (DeFronzo & Tripathy, 2009; Hsieh et al, 2018). As compared with the D and GS18 groups, the serum levels of TC, TG, and LDL‐C were significantly improved in the SS18‐5 group, which may have been due to the fact that bacteria can promote the excretion of free cholic acid and reduce serum cholesterol content and the absorption of cholesterol (Qian et al., 2019; Zeng et al.,2010). L .…”

Protective effect of Lactobacillus plantarum YS3 on dextran sulfate sodium‐induced colitis in C57BL/6J mice

“…qPCR mixture was prepared with 1 μl of SYBR Green PCR Master Mix (Thermo Fisher Scientific, Inc., Waltham, MA, USA), 2 μl of the obtained cDNA template, and 1 μl of both forward and reverse primer each (Table 2). Up to 40 qPCR cycles were performed after the following reaction conditions: 95°C, 60 s; then 95°C, 15 s; 55°C, 30 s; 72°C, 35 s; and 95°C, 30 s; 55°C, 35 s. With GAPDH as a reference parameter, the relative expression levels of target genes were determined by the following formula: 2 –ΔCT = ΔCT (detection gene) ‐ΔCT (GAPDH) (Qian et al., 2019).…”

“…. Up to 40 qPCR cycles were performed after the following reaction conditions: 95°C, 60 s; then 95°C, 15 s; 55°C, 30 s; 72°C, 35 s; and 95°C, 30 s; 55°C, 35 s. With GAPDH as a reference parameter, the relative expression levels of target genes were determined by the following formula: 2 -ΔCT = ΔCT (detection gene) -ΔCT (GAPDH)(Qian et al, 2019).…”