Effects ofLactobacillus plantarumon production performance, immune characteristics, antioxidant status, and intestinal microflora of bursin-immunized broilers

Abstract

Examples of probiotics that can promote host health by improving its intestinal microbial balance and intestinal immunity belong to the genus Lactobacillus. Bursin (BS) is a peptide isolated from the bursa of Fabricius for use as an adjuvant for a variety of immunogens. To investigate the synergistic effects of Lactobacillus plantarum (LP) dietary supplementation and BS immunization on production performance, immune characteristics, antioxidant status, and intestinal microflora in broilers, we randomly allocated 200 1-day-old broilers of mixed sex into 4 treatments in a 2 × 2 factorial arrangement (LP-/BS-, LP-/BS+, LP+/BS-, LP+/BS+) for 42 days. BS immunization enhanced immune response by increasing serum total immunoglobulin G concentration and interleukin-6 concentration, promoted antioxidant capacity by increasing catalase activities in serum and liver and by decreasing serum malondialdehyde (MDA) content at 42 days of age (DOA), and enriched intestinal microflora diversity. LP supplementation enhanced immune response by increasing interleukin-2 concentration at 42 DOA; promoted antioxidant capacity by increasing liver catalase activities, increasing glutathione peroxidase activities in serum and liver at 21 DOA, and decreasing serum MDA content at 42 DOA; promoted intestinal microflora composition by decreasing total aerobes and Escherichia coli counts at 21 DOA, by increasing total anaerobes count at 21 DOA, and by increasing Lactobacillus spp. and Bifidobacterium spp. counts at both 21 and 42 DOA. The interactions between BS and LP had a significant effect on daily body mass gain and feed conversion ratio in the starter period (1-21 DOA); on interleukin-2 concentration and liver MDA content at 21 DOA; and on thymus index, peripheral lymphocyte proliferation, and E. coli counts at 42 DOA. Overall, these data suggest that the combination of LP dietary supplementation and BS immunization promoted the production performance, immune characteristics, antioxidant status, and intestinal microflora of broilers.

Live Probiotic Lactobacillus johnsonii BS15 Promotes Growth Performance and Lowers Fat Deposition by Improving Lipid Metabolism, Intestinal Development, and Gut Microflora in Broilers

“…The enhanced ADG, slightly decreased FCR ( P > 0.05), and unchanged ADFI at the starter phase suggested that the FCR improvement might be due to improved nutrient digestibility. These results are similar to the findings reported by Shen et al (2014), who fed broilers with Lactobacillus plantarum . Moreover, the FCR was unchanged by LJ at days 22–42 and 1–42, although the ADG and ADFI were both increased.…”

“…In chickens, Shen et al (2014) found that Lactobacillus plantarum D22 could enhance the total count of anaerobes, as well as the individual counts of Lactobacillus spp. and Bifidobacterium spp.…”

“…To date, a number of probiotics have been used in the poultry industry. As extensively used probiotics in the poultry industry, Lactobacillus strains can promote growth performance (Nakphaichit et al, 2011; Shen et al, 2014), improve meat quality (Keokamnerd et al, 2007; Lv et al, 2012), enhance immune response (Huang et al, 2004; Stringfellow et al, 2011), and prevent some avian diseases (Pascual et al, 1999; Yamazaki et al, 2012; Youn et al, 2012). However, it has proved difficult to demonstrate the mechanism whereby probiotics affect the gastrointestinal tract because probiotic strains exert their beneficial effects via different mechanisms in which other microbiota may be involved as well (Rubio et al, 2014; Serban, 2014; Patten and Laws, 2015).…”

Antioxidative activity of probiotics

“…Lactobacillus fermentum has a beneficial influence on antioxidant activity, thereby also on pig health: it has been reported to increase SOD and glutathione peroxidase (GPx) activity, and contribute to a decrease in MDA level in pig serum [40], and hepatic CAT acidity was elevated after probiotic administration. It was also found that L. plantarum administration contributes to an increase in the activity of antioxidant enzymes in broiler chicken liver (GPx and CAT) and serum (GPx) and a decrease in MDA level in the serum [41].…”

Lactobacillus plantarum BS22 promotes gut microbial homeostasis in broiler chickens exposed to aflatoxin B1

“…Lactobacillu s is one of the most studied probiotics in binding the AFB 1 in poultry (El‐Nezami, Kankaanpaa, Salminen, & Ahokas, ; Gratz, Mykkänen, & Elnezami, ; Hernandez‐Mendoza, Guzman‐De‐Peña, Vallejo‐Córdoba, & Garcia, ; Hamidi et al., ). In addition, the benefits of Lactobacillus plantarum BS22 (LP) on immune characteristics, production performance and intestinal microflora in healthy broiler were confirmed (Shen et al., ). PCR‐DGGE provides an easy and intuitive approach to analyse microbiota (Chen, Wang, Jiang, Xiong, & Wei, ).…”