Effects of Imidazole- and Triazole-derivative Antifungal Compounds on the Growth and Morphological Development of Candida albicans Hyphae

Odds, Frank C.; Hayward, Janis; Abbott, A.B.

doi:10.1099/00221287-131-10-2581

Cited by 51 publications

(54 citation statements)

References 17 publications

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“…Most publications describing inhibition of GTF do not mention the morphologies of those cells not bearing germ tubes, therefore comparisons are limited. However, Odds et al [27] note that in the presence of azoles, development of hyphae was severely restricted and that growth occurred in the form of clumped budding yeast cells.…”

Section: Discussionmentioning

confidence: 99%

Melaleuca alternifolia (tea tree) oil inhibits germ tube formation by Candida albicans

Hammer¹,

Carson²,

Riley³

2000

Med. Mycology

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The effect of tea tree oil (TTO) on the formation of germ tubes by Candida albicans was examined. Two isolates were tested for germ tube formation (GTF) in the presence of TTO concentrations (% v:v) ranging from 0·25% ( 1 2 minimum inhibitory concentration [MIC]) to 0·004% (1:128 MIC). GTF at 4 h in the presence of 0·004 and 0·008% (both isolates) and 0·016% (one isolate) TTO did not differ signi cantly (P \ 0·05) from controls. At all other concentrations at 4 h, GTF differed signicantly from controls (P B 0·01). A further eight isolates were tested for GTF in the presence of 0·031% TTO, and at 4 h the mean GTF for all 10 isolates ranged 10·0 -68·5%. Two isolates were examined for their ability to form germ tubes after 1 h of pre-exposure to several concentrations of TTO, prior to induction of germ tubes in horse serum. Cells pre-exposed to 0·125 and 0·25%TTO formed signi cantly fewer germ tubes than control cells at 1 h (P B 0·05), but only those cells pre-exposed to 0·25% differed signi cantly from control cells at later time points (P B0·01). GTF by C. albicans is affected by the presence of, or pre-exposure to, sub-inhibitory concentrations of TTO. This may have therapeutic implications.

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Section: Discussionmentioning

confidence: 99%

Melaleuca alternifolia (tea tree) oil inhibits germ tube formation by Candida albicans

Hammer¹,

Carson²,

Riley³

2000

Med. Mycology

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“…(g body weight) − ". For examination of C. albicans morphology in vitro, yeasts were grown overnight in CYGi broth at 30 mC with constant rotation and cell suspensions were diluted to 1-5i10& yeast cells ml − " in pre-warmed volumes of foetal calf serum, buffered Eagle's modified essential medium (EMEM ; Odds et al, 1985) or modified Sabouraud broth (MSAB ; Evans et al, 1975). These cultures were incubated at 37 mC (serum and EMEM) or 40 mC (MSAB).…”

Section: Methodsmentioning

confidence: 99%

Survival in experimental Candida albicans infections depends on inoculum growth conditions as well as animal host

2000

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Evidence is presented that the growth medium used to prepare a Candida albicans challenge inoculum is a significant factor determining the ability of a fungus strain to gain an initial invasive hold immediately after injection into an animal host, and thus determining gross strain lethality. Three C. albicans strains, one known to be attenuated in virulence, were grown in two broth media and injected intravenously at different doses into female NMRI mice and male albino guinea pigs. For each fungus strain and challenge dose, survival was longer from inocula grown in a diluted, buffered peptone-based broth than from inocula grown in Sabouraud glucose broth. When animals were challenged intravenously with yeast doses adjusted to give the same mean survival time regardless of strain or growth medium, the progression of fungus tissue burdens (c.f.u. g N1 ) in kidneys, lungs, liver, spleen and brain samples was broadly similar for all three C. albicans strains but differed between the two animal hosts. The morphological form of C. albicans recovered from infected tissues differed at the level of both the fungus strain and the host tissue. Use of survival-standardized inocula provides a means of distinguishing differences in progression of experimental disseminated Candida infections that are related to the infecting strain from those related to the animal host.

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“…In other studies, adenosine 5'-triphosphate (ATP) levels in cells of C. albicans were suppressed (203), enzyme-catalyzed release of spheroplasts from young yeast cells of C. albicans was inhibited (13), and hyphal development in T. mentagrophytes (258) and C. albicans was prevented (205).…”

Section: Clotrimazolementioning

confidence: 99%

Overview of medically important antifungal azole derivatives

1988

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Fungal infections are a major burden to the health and welfare of modern humans. They range from simply cosmetic, non-life-threatening skin infections to severe, systemic infections that may lead to significant debilitation or death. The selection of chemotherapeutic agents useful for the treatment of fungal infections is small. In this overview, a major chemical group with antifungal activity, the azole derivatives, is examined. Included are historical and state of the art information on the in vitro activity, experimental in vivo activity, mode of action, pharmacokinetics, clinical studies, and uses and adverse reactions of imidazoles currently marketed (clotrimazole, miconazole, econazole, ketoconazole, bifonazole, butoconazole, croconazole, fenticonazole, isoconazole, oxiconazole, sulconazole, and tioconazole) and under development (aliconazole and omoconazole), as well as triazoles currently marketed (terconazole) and under development (fluconazole, itraconazole, vibunazole, alteconazole, and ICI 195,739).

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Effects of Imidazole- and Triazole-derivative Antifungal Compounds on the Growth and Morphological Development of Candida albicans Hyphae

Cited by 51 publications

References 17 publications

Melaleuca alternifolia (tea tree) oil inhibits germ tube formation by Candida albicans

Melaleuca alternifolia (tea tree) oil inhibits germ tube formation by Candida albicans

Survival in experimental Candida albicans infections depends on inoculum growth conditions as well as animal host

Overview of medically important antifungal azole derivatives

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