Effects of in vivo mycorrhization on micropropagated fruit tree rootstocks

Monticelli, S.; Puppi, G.; Damiano, C.

doi:10.1016/s0929-1393(00)00085-8

Cited by 35 publications

(19 citation statements)

References 24 publications

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“…Similarly, survival increase of tissue-cultured saplings by inoculating AM fungi is documented (Monticelli et al 2000;Yano-Melo et al 2003). The two main deficiencies of in vitro grown plants are: 1) a poor control of water loss, and 2) heterotrophic mode of nutrition (Bhojwani, Razdan 1996).…”

Section: Effect Of Nacl On Shoot and Root Developmentmentioning

confidence: 99%

“…Mycorrhizal symbiosis resulted in significant changes in root system morphology, acclimatization and transplant survival in micropropagated plants (Monticelli et al 2000;Yano-Melo et al 2003). It plays an important role in water economy of plants by improving the hydraulic conductivity of the roots and contributing towards a better uptake of water by the plants even in extremely dry conditions (Al-Karaki et al 2001).…”

Section: Effect Of Seasonsmentioning

confidence: 99%

“…In this context, the use of in vitro methods may prove beneficial as the plant tissue culture techniques take a considerably shorter period of time, reduce time between generations and generate large variations, which is useful for inducing the desired traits. The inoculation of tissue cultured plantlets with suitable bioinoculants like Arbuscular Mycorrhiza (AM) fungi (Monticelli et al 2000;Yano-Melo et al 2003) and Azotobacter (Carletti 2000) could not only protect them from transplant shocks and lower their total mortality percentage but also improve the biomass quality. The inoculation of plant roots with AM fungi helps in nutrient recycling, water absorption, production of phytohormones, biocontrolling of pathogens, stress tolerance and soil fertility improvement (Sharma et al 1997).…”

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confidence: 99%

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In vitro selection of salt tolerant Morus alba and its field performance with bioinoculants

Kashyap¹,

Sharma²

2006

Hort. Sci. (Prague)

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ABSTRACT:In vitro selected salt tolerant saplings of Morus alba (cv. Sujanpuri) were raised from nodal explants with axillary buds collected during three different periods of the year. The growth and shoot/root multiplication of the nodal explants collected between November to February and July to October were found to be better than those collected between March to June. In cultures, shoot multiplication was induced by the application of 2.5 mg/l of 6-benzylaminopurine (BAP) and 0.3 mg/l of gibberellic acid (GA 3 ), while rooting by 1.0 mg/l of indolebutyric acid (IBA). Sodium chloride (NaCl) was added to induce salt stress and its concentration was gradually increased from 0.1% (w/v) onwards. The salt tolerance was observed up to 0.4% (w/v) NaCl and 100% mortality of explants was noted above this concentration. The inclusion of Arbuscular Mycorrhizal (AM) fungi and Azotobacter chroococcum to tissue culture of raised saplings during acclimatization enhanced their survival and resulted in a significant increase of plant growth. After the transfer of plants to salt affected wasteland, only NaCl-treated saplings survived, whereas those developed without NaCl resulted in 100% mortality. plying salt (NaCl) stress and if exploiting AM fungi and Azotobacter during hardening could enhance the survival percentage of developed plants on salt affected land. Keywords: in vitro; MATERIALS AND METHODSExplant collection: Growing shoots of Morus alba L. cv. Sujanpuri were collected from one year old mulberry plants cultivated on normal soil (pH = 7.5, EC = 0.110 mmhos/cm) at Micromodel, IIT, Delhi during the following months: A) March to June, B) July to October, C) November to February. Nodal segments with one axillary bud each (1.5 to 2.0 cm) were excised, washed and disinfected first with 0.7% (w/v) bleach solution (sodium hypochloride) for 10 min and with 0.1% aqueous mercuric chloride solution for next 10 minutes and then rinsed 5 to 6 times with sterile distilled water.Shoot multiplication: The Murashige and Skoog's (MS) (1962) medium containing 3% (w/v) sucrose, 2.5 mg/l of BAP and 0.3 mg/l of GA 3 with 1.0% agar and pH 5.8 was used as shoot multiplication medium. For induction of salinity stress, the medium was supplemented with 0.1% NaCl. The surface sterilized explants were inoculated vertically onto the culture medium with one explant per test tube and twenty test tubes were kept for each treatment.Cultures were grown under cool white fluorescent tubes with irradiance of 24 µmol m 2 /s, 16 h photoperiod and at the temperature of 25 ± 1°C. After multiple shoot induction, the NaCl concentration of medium was gradually increased from 0.1 to 0.4% till 100% mortality of explants observed and average shoot length and number of shoots per explant was noted.Root induction: For rhizogenesis, well developed shoots (5.0 cm long) with 2-3 leaves were excised from cultures and cultured on MS supplemented with 1.0 mg/l of IBA, 3.0% sucrose and NaCl (from 0.1 to 0.4%). For root production, shoots were inoculated on medium with the...

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Section: Effect Of Nacl On Shoot and Root Developmentmentioning

confidence: 99%

Section: Effect Of Seasonsmentioning

confidence: 99%

mentioning

confidence: 99%

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In vitro selection of salt tolerant Morus alba and its field performance with bioinoculants

Kashyap¹,

Sharma²

2006

Hort. Sci. (Prague)

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“…A lot of research has been carried out to limit the consequences of this phenomenon in orchards (Bechmer et al, 2003;Kowalik et al, 1998;Merwin and Byard, 2001;Reginato et al, 2008;Szczygieł and Zepp, 1998;Tustin et al, 2008;Zydlik, 2004), but fewer studies concern nurseries (Porębski et al, 2003;Simeone et al, 2006). Recently, studies on the use of mycorrhizal inoculum to limit the 'disease' have been carried out (Caruso et al, 1989;Catska, 1994;Catska and Taube--Baab, 1994;Druzic-Orlic et al, 2008;Monticelli et al, 2000;Rutto and Mizutani, 2006).…”

Section: Introductionmentioning

confidence: 99%

Maiden pear trees growth in replant soil after inoculation of rootstocks with mycorrhizal inoculum

Świerczyński

Stachowiak

Golcz-Polaszewska

2015

Nauka Przyr. Technol.

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Summary. In the production of fruit trees it is important to set up a nursery always in a new site, alternatively to follow crop rotation rules. It is not always possible due to the size of a farm and production volume. In order to limit the effects of replant soil one can use different procedures before setting up a nursery. Chemical methods, however, must be replaced with non chemical ones due to environmental protection and reduction of production costs. In the experiment conducted in 2009-2012, growth of maiden pear trees of 'Conference' growing on three quince MA, MC, S1 rootstocks, cultivated in replant and non-replant soil after the use of mycorrhizal treatment, was compared. The strongest growth of maiden pear trees was obtained on non-replant soil with mycorrhizal and without mycorrhizal treatment of rootstocks. Inoculation of rootstocks influenced positively the height and fresh mass of the root system of maiden pear trees growing on two considered sites. On the other hand, inoculation did not rise the diameter of stem and number of lateral shoots of the maidens. Influence of mycorrhizal treatment of rootstocks on the length of lateral shoots was not obvious. Significantly the best results of maiden pear trees growth, except for the stem diameter, were obtained on MA quince compared to two other types. The mycorrhizal treatment gave better result of percentage obtained by maiden pear trees only in the replant site. The best efficiency of maiden pear trees in nursery production was observed for MA quince rootstock.

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“…The results suggest an interaction between substrate composition, fertilisation and AMF inoculation, as previously reported for micropropagated woody plants (Estaún et al, 1999), since G. deserticola and G. intraradices only improved growth of plants previously cultivated with lower amounts of fertiliser in high-and lowpeat substrates, respectively. Differences in growth promotion of micropropagated plants have been reported for different species of perennial plants (e.g., Monticelli et al, 2000;Taylor and Harrier, 2000;Yano-Melo et al, 1999). Main stem and lateral branch growth was also differentially affected by substrates and AMF inoculation.…”

Section: Resultsmentioning

confidence: 85%

Enhanced growth of wild cherry using micropropagated plants and mycorrhizal inoculation

Lovato

Trouvelot

Gianinazzi‐Pearson

et al. 2006

Agron. Sustain. Dev.

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-Mycorrhizal inoculation is a promising, sustainable technique to enhance plant growth. We evaluated the effects of mycorrhizal inoculation and of the use of two substrates, soil and peat, on the growth of wild cherry, Prunus avium L., on the weaning and post-weaning. After weaning, plants were grown for 13 weeks in a greenhouse on either 40% soil or 40% peat at two levels of fertiliser: 2 or 4 g m -3 of a 16:9:12 slow-release fertiliser. They were subsequently kept for a further 120 days in a frost-free greenhouse before outplanting to the field. The results show that enhanced plant growth after seven months in the field was associated with increased peat and fertiliser levels in the substrates during the post-weaning growth phase, and with prior mycorrhizal inoculation by a G. deserticola isolate, which compensated for less favourable substrate conditions. Plants inoculated with G. intraradices had more branches positioned in the lower half of the stem, while plants inoculated with G. deserticola had more branches in the upper half of the stem. arbuscular mycorrhiza / plant architecture / wild cherry / field outplanting

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Effects of in vivo mycorrhization on micropropagated fruit tree rootstocks

Cited by 35 publications

References 24 publications

In vitro selection of salt tolerant Morus alba and its field performance with bioinoculants

In vitro selection of salt tolerant Morus alba and its field performance with bioinoculants

Maiden pear trees growth in replant soil after inoculation of rootstocks with mycorrhizal inoculum

Enhanced growth of wild cherry using micropropagated plants and mycorrhizal inoculation

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