Effects of Indigenous Spore-Forming Probiotic as Feed Supplement on Performance and Safety in Broilers

Abstract

Probiotics colonize the intestine of animals and birds and provide useful effects on their performance and immune status. This study describes a high throughput screening and characterization of spore-forming bacteria from Iranian poultry farms with the aim to identify potential probiotic native Bacillus spp. and determine its effects on growth performance, hemato-biochemical parameters, immunity, intestinal microflora, morphology and MUC2 gene expression of broiler chickens. A total of 300 one-day-old female Ross 308 broilers (42.6 ± 0.6 g) were used in a 6-wk study. Broilers were randomly allotted to 1 of 3 dietary treatments consisting of 4 replicate cages with 25 broilers each: 1-Control (Corn-soy-based diet: C), 2-C + 200 g/ton of the GalliPro® (Bacillus subtilis DSM 17299, 4×10 9 CFU/g, as positive control group: PC), 3-C + 200 g/ton of the native probiotic (B. tequilensis K03, 4×10 9 CFU/g: NP) identified in this study. During the experiment parameters were measured weekly. The results revealed that birds of the NP and PC groups exhibited improved feed conversion ratio (FCR) and increased body weight (BW), carcass and breast meat yield compared with the birds of the C group (P<0.05). Also, lymphocytes level, antibody titers against Newcastle diseases virus (NDV) and infectious bronchitis virus (IBV) of vaccinated birds were increased, while serum triglycerides, total cholesterol levels and abdominal fat of birds fed NP and PC were decreased compared to birds of the C group (P<0.05). The villus height, the relative expression of MUC2 gene and Bacillus spp. populations were increased, while E. coli was significantly decreased in the ileum content of treated groups (P<0.05). These results indicate that the identified native B.tequilensis K03 strain can improve immunity and broiler performance by modifying intestinal microflora and morphology. Studied native probiotic Bacillus tequilensis K03 has useful effects on health status and it can be used as poultry feed supplement.

Preventive antimicrobial action and tissue architecture ameliorations of Bacillus subtilis in challenged broilers

“…The observed actions could be attributed to the enhancing influence of B. subtilis probiotics on intestinal permeability and absorption functions. The results were consistent with those of He et al [50] and Hosseini et al [51], who reported that the inclusion of probiotics in broiler chicken rations could improve nutrient digestion, performance, antioxidant levels and activity, and intestinal morphology barriers against pathogenic microorganisms. Sobczak and Kozłowski [52] recorded synchronized results and found that B. subtilis (1×10 8 CFU/kg feed) improved performance, egg quality, and yolk cholesterol contents.…”

Dietary Probiotics Modulate Gut Barrier and Immune-Related Gene Expression and Histomorphology in Broiler Chickens under Non- and Pathogen-Challenged Conditions: A Meta-Analysis

“…The following supporting information can be downloaded at: , Table S1: List of references and the respective experimental variables included in the meta-analysis [ 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 ]; Table S2: Descriptive statistics for predictive and response variables of jejunal and ileal gene expression (fold change) related to barrier function and immune response in broiler chickens at weeks 3 and 6 of life without pathogen challenge; Table S3: Descriptive statistics for predictive and response variables of jejunal and ileal histomorphology (fold change) in broiler chickens at weeks 3 and 6 of life without pathogen challenge; Table S4: Descriptive statistics for predictors and response variables of growth performance in broiler chickens at starter, finisher, and overall periods without pathogen challenge; Table S5: Descriptive statistics for predictive and response variables of jejunal, ileal, and cecal gene expression (fold change) related to barrier function in broiler chickens from weeks 2 to 5 with pathogen challenge; Table S6: Descriptive statistics for predictive and response variables of jejunal, ileal, and cecal gene expression (fold change) related to immune response in broiler chickens from weeks 2 to 4 of life with pathogen challenge; Table S7: Descriptive statistics for predictive and response variables of duodenal, jejunal and ileal histomorphology (fold change) in broiler chickens at weeks 2, 3, and 5 of life with pathogen challenge; Table S8: Descriptive statistics for predictors and response variables of growth performance in broiler chickens at starter, finisher, and overall periods with pathogen challenge.…”