Effects of infrared laser radiation on qualitative and quantitative indices of a regenerating skeletal muscle in the age aspect

Gallyamutdinov, R. V.; Астахова, Л. В.; Головнева, Е. С.; Serysheva, O. U.

doi:10.37895/2071-8004-2020-24-2-3-90-94

Cited by 3 publications

(1 citation statement)

References 4 publications

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“…Тренировочный процесс представлял собой плавательную нагрузку в резервуаре емкостью 200 л, толщина слоя воды составляла 0,5 м, температура воды -+22-23 °С. Крысы помещались в указанный резервуар 3 раза в неделю, длительность нагрузки увеличивали каждую неделю на 5 минут (с 30 до 55 минут) [7]. Лимфоциты -источники морфогенетически активной ксеногенной РНК выделяли путем суспендирования селезенки 30-дневного поросенка в стеклянном гомогенизаторе с последующей фильтрацией взвеси через капроновый фильтр и 3-кратным центрифугированием клеток в стерильном 0,9% растворе NaCl.…”

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Xenogeneic lymphocytic RNA stimulates skeletal muscle regeneration

Tishevskaya

Головнева

Gallyamutdinov³

et al. 2021

RJTAO

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Objective: to find evidence of the existence of distant lymphocytic RNA control of physiological myogenesis as a way to control the muscle tissue regeneration process.Materials and methods. The study was conducted on male Wistar rats, n=33. In the first part of the experiment, 12 rats were subjected to regular 40-day physical activity (swimming), half of them were intraperitoneally injected 4 times with total RNA isolated from pig spleen lymphocytes at 30 days of age; 6 rats made up the intact control group. In histological preparations of different skeletal muscle groups, the width and cross-sectional area of muscle fibers, the area of nuclei, and the number of myocytes and myosatellite cells were evaluated. In the second part of the experiment, 15 intact rats were injected with the studied xenogeneic RNA and the amounts of ribonucleic acids in peripheral blood lymphocytes, spleen lymphocytes, and skeletal muscles were determined 2 hours and 24 hours after injection.Results. After the 40- day physical activity, the width of the fibers and the area of myocyte nuclei in the skeletal muscles increased; the absolute number of myosatellite cells and the area of their nuclei did not change. After administration of xenogenic RNA in the trained rats, in addition to an increase in the thickness and cross-sectional area of muscle fibers, the absolute number of myosatellite cells in m. biceps femoris, in m. triceps brachii, and in m. pectoralis major increased 1.4-fold, 1.3-fold, and 1.4-fold, respectively; the area of myosatellite nuclei increased on average by 7%. In intact rats, two hours after xenogeneic RNA injection, the amount of RNA in skeletal muscles remained unchanged, it increased by 19% in spleen lymphocytes, and by 16% in peripheral blood lymphocytes. At 24 hours, the RNA amount in the lymphocytes remained significantly higher than the control values, while in the muscle tissue, it didn’t differ from the control.Conclusion. Xenogeneic lymphocytic RNA stimulates physiological myogenesis by activating myosatellite cell proliferation.

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Xenogeneic lymphocytic RNA stimulates skeletal muscle regeneration

Tishevskaya

Головнева

Gallyamutdinov³

et al. 2021

RJTAO

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Effect of Infrared and Green Photomodulation Exposure on the Number of Active Myosatellite Cells in Regenerating Muscles

Tahaviev,

Golovneva,

Bryukhin

2024

Bull Exp Biol Med

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Infrared laser exposure in combination with branchedchain amino acid stimulates physiological adaptation of skeletal muscles

Gallyamutdinov¹,

Головнева

Revel-Muroz³

et al. 2022

Lazernaâ medicina

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Laser exposure stimulates cell proliferation and tissue repair. Branched-chain amino acids (BCAA) are widely used in sports medicine as a stimulator of anabolic processes. However, there is no evidence of the effect of combined laser and BCAA application on skeletal muscle morphometric characteristics during exercise in the training process.Purpose: to study the effect of infrared laser exposure in combination with amino acid at myosatellitocytes and skeletal muscle myocytes during swimming training.Material and methods. The experiment was conducted on 30 Wistar rats: Group 1 – intact, Group 2 – dynamic control (trainings with endurance swimming), Group 3 – trainings and BCAA, Group 4 – trainings and laser exposure at hip muscles (970 nm, 1 W, 60 s), Group 5 – combined exposure to laser and BCAA. Trainings were three times a week for 6 weeks; laser session was after each training. Samples of the bipedal thigh muscle were ﬁxed with formalin; histological sections were stained with hematoxylin-eosin. The morphometric analysis of the digital image of objects with statistical processing by Mann – Whitney method was made.Results. Laser exposure combined with BCAA during trainings increased the nuclei area and the number of myosatellites and myocytes; it also enlarged the cross section of muscle ﬁbers which was more pronounced if to compare with isolated laser irradiation of the muscle or BCAA.Conclusions. Infrared laser exposure in combination with branched-chain amino acids effectively stimulates regeneration due to hyperplasia and hypertrophy of skeletal muscular tissue, thus providing physiological adaptation in the training process.

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Effects of infrared laser radiation on qualitative and quantitative indices of a regenerating skeletal muscle in the age aspect

Cited by 3 publications

References 4 publications

Xenogeneic lymphocytic RNA stimulates skeletal muscle regeneration

Xenogeneic lymphocytic RNA stimulates skeletal muscle regeneration

Effect of Infrared and Green Photomodulation Exposure on the Number of Active Myosatellite Cells in Regenerating Muscles

Infrared laser exposure in combination with branchedchain amino acid stimulates physiological adaptation of skeletal muscles

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