Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (Silurus triostegus, H. 1843) Sperm

Abstract

This study was based on the determination of post-thaw semen quality, oxidant–antioxidant status and DNA-damage status of frozen Mesopotamian catfish semen. To this end, the sperm was frozen in diluent containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly increased the spermatozoa motility rate and duration significantly (p < 0.05). High doses of inositol increased antioxidants and decreased oxidants. In addition, lower intracellular DNA damage and percentage of apoptotic spermatozoa occurred in high doses of inositol (p < 0.05).Abstract: In this study, the effects of supplemented inositol on sperm extenders were examined on the spermatozoa motility rate and duration, total antioxidant and oxidant status, apoptotic spermatozoa and DNA damage, during the sperm post-thaw process of Mesopotamian Catfish (Silurus triostegus, H. 1843). The semen was frozen in diluents containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly improved the spermatozoa motility rate and duration significantly (p < 0.05). MDA and TOS were linearly decreased, however, TAS and GSH linearly increased (p < 0.05). The increasing inositol levels resulted in a linear and quadratic decrease in DNA damage in the comet assay, 8-hydroxydeoxyguanosine and the determined percentage of apoptotic spermatozoa (p < 0.05). These results suggest that there are many positive effects of the use of supplemental inositol on enhancing sperm cryopreservation efficiency in Silurus triostegus.

Antioxidant Effects of Myo-Inositol Improve the Function and Fertility of Cryopreserved Boar Semen

“…In contrast, SMCP was significantly increased in the myo-inositol group compared to the control group. This result is consistent with previous studies in human and animal sperm, showing that myo-inositol treatment in cryopreserved semen decreased oxidative stress and apoptosis, increased the cryosurvival rate, and improved gene expression [ 24 , 26 , 54 , 65 , 67 , 68 ].…”

“…The freeze–thaw process is stressful to sperm, and during this process, sperm confront prolonged stress and increase ROS production, which leads to DNA fragmentation, changes in membranes, and cell apoptosis. Multiple studies report strong beneficial effects of myo-inositol on protecting sperm DNA and reducing ROS and apoptosis during cryopreservation [ 25 , 65 , 66 ]. Our study investigated the effect of different myo-inositol concentrations on boar sperm and evaluated oxidative stress and apoptosis parameters, including caspase activity, apoptosis index, ROS levels, and related genes, as shown in Figure 2 C, Figure 3 , and Figure 5 B.…”

Role of antioxidants in fertility preservation of sperm — A narrative review

“…In another study, Condorelli et al reported that MYO enhanced the motility of sperm retrieved following the swim-up procedure in both fertile and infertile individuals [129]. Furthermore, an MYO-supplemented freezing extender showed reduced OS and improved freeze-thaw sperm quality in different species including dogs [130], fish [131], bucks [132], and humans [133]. Similar results were observed when MYO supplementation was used during thawing procedures [134].…”