Background: Nutrient balance in rice paddy fields can change under different tillage management practices; however, the extent of the change may differ for various rice varieties. A field experiment was conducted over 2 successive years from June 2016 to June 2018 to clarify the effects of different tillage methods and rice varieties on nutrients remaining in the surface soil and those taken up by the rice. There were four tillage/rice variety treatments: no-till/japonica rice (NTJ), no-till/glutinous rice (NTG), conventional tillage/japonica rice (CTJ), and conventional tillage/glutinous rice (CTG) with three replicates of each.Results: The tillage method and rice variety had interactive effects on the surface balance of nitrogen (N) and phosphorus (P). After the 2-year experiment, total N and P pools in the 20 cm surface soil layer were highest under the NTJ treatment (4757.0 kg N ha-1, 2428.5 kg P ha-1) and lowest under the CTG treatments (4726.3 kg N ha-1, 2412.0 kg P ha-1). Under no-till conditions, japonica rice had higher grain yield but lower straw biomass than that of glutinous rice. NTG treatment significantly increased the N and P content in rice grains and straw. The environmental losses of N and P in the rice fields mainly occurred during the rice season. Based on nutrient balance estimation, N losses in the NTJ, NTG, and CTJ treatments were reduced by 15.03 kg N ha-1, 11.55 kg N ha-1, 6.72 kg N ha-1, respectively, as compared with that of the CTG treatment. The corresponding P losses were reduced by 8.02 kg P ha-1, 7.84 kg P ha-1, and 3.96 kg P ha-1. In terms of economic benefits, NTJ also resulted in the highest actual income (18896.5 yuan ha-1) and the highest ratio of production to investment (6.40).Conclusion: No-till was more likely to maintain soil N and P nutrients than conventional tillage, and this advantage was most significant when planting japonica rice. In addition, no-till was conducive to N and P uptake by rice, but only japonica rice exhibited increased grain yield. Consequently, the NTJ treatment was recommended.