The decline in rice yields as a result of excessive chemical fertilizer (CF) inputs is a matter of great concern in rice-growing regions of Asia. In two-year’s field experiments, the nitrogen, phosphorus, and potassium (NPK) status, growth characteristics and yield of rice were examined by application of poultry manure (PM), cow manure (CM) and compost (CP). Organic fertilizers were applied as EMN (estimated mineralizable N) based on their total N content. Six treatments were assigned in a randomized complete block design: (1) no-N fertilizer (N0); (2) 50% CF (CF50), (3) 100% CF (CF100); 50% CF + 50% EMN from (4) PM or (5) CM or (6) CP. Compared with CF100, the CF50PM50 (total N ≥ 4%) accumulated higher N, P and K content in leaf, sheath, panicle and seeds, resulting in greater growth and yield. The CF50PM50 increased yield by 8.69% and 9.70%, dry matter by 4.76% and 5.27% over CF100 in both years. The continuous application of CF50CM50 (total N < 4%) and CF50CP50 (total N < 4%) treatments led to similar NPK contents but higher yields than those of CF100 treatment in 2018. In conclusion, the organic fertilizer (total N ≥ 4%) with the EMN method enhances higher N availability in each year. Continuous application of organic fertilizer (total N < 4%) over two years effectively increased N availability in the second year. The 50% organic fertilizer (total N ≥ 4%) and 50% CF led to increased NPK availability and rice yields over the 100% CF treatment, reducing CF usage and leading for sustainable agriculture.

No abstract

The use of biofertilizers is important for sustainable agriculture, and the use of nodule bacteria and endophytic actinomycetes is an attractive way to enhance plant growth and yield. This study tested the effects of a biofertilizer produced from Bradyrhizobium strains and Streptomyces griseoflavus on leguminous, cereal, and vegetable crops. Nitrogen fixation was measured using the acetylene reduction assay. Under N-limited or N-supplemented conditions, the biofertilizer significantly promoted the shoot and root growth of mung bean, cowpea, and soybean compared with the control. Therefore, the biofertilizer used in this study was effective in mung bean, cowpea, and soybean regardless of N application. In this study, significant increments in plant growth, nodulation, nitrogen fixation, nitrogen, phosphorus, and potassium (NPK) uptake, and seed yield were found in mung beans and soybeans. Therefore, Bradyrhizobium japonicum SAY3-7 plus Bradyrhizobium elkanii BLY3-8 and Streptomyces griseoflavus are effective bacteria that can be used together as biofertilizer for the production of economically important leguminous crops, especially soybean and mung bean. The biofertilizer produced from Bradyrhizobium and S. griseoflavus P4 will be useful for both soybean and mung bean production.