Effects of intraduodenal nutrient infusion on insulin response to subsequent intravenous glucose in rats

Ikeda, Tadasu; Yoshida, Tazue; Honda, M.; Ito, Yasuo; Mokuda, O; Tominaga, Masato; Mashiba, Hiroto

doi:10.1016/0026-0495(87)90136-3

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…Goldberg et af. ( 5 ) , Shima et al (6), and we (7) have reported that ingestion of a non-nutrient drink augments insulin secretion in response to a subsequent intravenous glucose load in humans and rats, suggesting that intestinal mechanical stimulation and/or osmolality may play a component role in insulin release. However, the effect of intestinal osmolality on insulin secretion remains to be further elucidated.…”

Section: Tinal Factors and Several Investigators ( 1-4) Havementioning

confidence: 91%

Effect of Intestinal Osmolality on Insulin Secretion to Subsequent Intravenous Glucose or Arginine in the Rat

Ikeda

Fujiyama²,

Hoshino³

et al. 1990

Experimental Biology and Medicine

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To elucidate the effect of intestinal osmolality on insulin secretion, we investigated insulin response to a subsequent intravenous infusion of glucose or arginine after intragastric or intraduodenal mannitol or NaCl instillation in the rat. After anesthesia with intraperitoneal pentobarbital sodium, mannitol solution (10% or 20%) or 2.7% NaCl was instillated into the stomach or duodenum for 5 min at a flow rate of 0.5 ml/min, and 20% glucose (0.5 g/kg) or 10% L-arginine (0.5 g/kg) was infused bolus into the femoral vein 45 min after intestinal instillation. Insulin response to intravenous glucose was significantly higher in the rat with intragastric or intraduodenal mannitol or NaCl infusion than in control rats with intragastric or intraduodenal instillation of distilled water. Insulin response to intravenous arginine was almost the same in all groups. Subcutaneous preadministration of propranolol (0.4 mg/kg), atropine (1.2 mg/kg), or phentolamine (0.8 mg/kg) did not alter the present phenomenon. These results suggest that intestinal osmolality may enhance insulin release to intravenous glucose, but not to arginine in the rat.

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Section: Tinal Factors and Several Investigators ( 1-4) Havementioning

confidence: 91%

Effect of Intestinal Osmolality on Insulin Secretion to Subsequent Intravenous Glucose or Arginine in the Rat

Ikeda

Fujiyama²,

Hoshino³

et al. 1990

Experimental Biology and Medicine

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“…This is consistent with a relative temporal order of digestion and passage from the stomach and absorption via the intestinal tract. However, it is also plausible that the differences seen in the macronutrient stimulation of both insulin and GLP-1 at the 90min point may also be attributed to how stimulatory these macronutrients are upon the different endocrine cell populations involved [44][45][46].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Fat, carbohydrate and protein by oral gavage in the rat can be equally effective for satiation

Cassie

Anderson

Wilson

et al. 2019

Physiology & Behavior

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This study aimed to determine the relative efficacy of the macronutrients, protein, fat and carbohydrate to induce satiation and satiety in rats in relation to macronutrient activation of neurons in the nucleus of the solitary tract (NTS). Male Sprague Dawley rats were schedule-fed twice a day for 2h, receiving 100% of daily ad-libitum energy intake. On test day 1, 30min before the first scheduled meal of the day, rats were gavaged with an 8kcal isocaloric, isovolumetric solution of a glucose, lipid or peptone macronutrient solution or a non-caloric saline solution. To assess satiation, thirty minutes later rats were given access to food for 2h and food intake determined. A second 2h food access period 3h later was used for assessment of satiety. On the second test day, rats were gavaged as before and killed 90min after food presentation. Blood was collected for measurement of circulating metabolic markers. Brains were removed for analysis of c-Fos expression by in situ hybridization in the NTS.Rats which received saline consumed a similar amount of food compared to pre-gavage intakes.However, rats gavaged with a caloric macronutrient solution all reduced food intake by 18-20kcal. Interestingly, the reduction in caloric intake was greater than the caloric value of the macronutrient solution gavaged and was sustained following the second scheduled meal. Quantification by in situ hybridization of c-Fos mRNA expression in the NTS 90 minutes post-gavage, showed a significant increase with each macronutrient, but was 24-29% higher with a lipid or peptone gavage compared to a glucose gavage. In conclusion, when delivered directly to the stomach, all macronutrients can be equally effective in inducing satiation with significant neuronal activation in the NTS of the hindbrain.

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Intraduodenal osmolality directly enhances insulin secretion in the rat

et al. 1992

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Effects of intraduodenal nutrient infusion on insulin response to subsequent intravenous glucose in rats

Cited by 4 publications

References 29 publications

Effect of Intestinal Osmolality on Insulin Secretion to Subsequent Intravenous Glucose or Arginine in the Rat

Effect of Intestinal Osmolality on Insulin Secretion to Subsequent Intravenous Glucose or Arginine in the Rat

Fat, carbohydrate and protein by oral gavage in the rat can be equally effective for satiation

Intraduodenal osmolality directly enhances insulin secretion in the rat

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