2009
DOI: 10.1152/ajpendo.90497.2008
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Effects of intrafetal IGF-I on growth of cardiac myocytes in late-gestation fetal sheep

Abstract: Intrafetal insulin-like growth factor (IGF)-I promotes cardiac hypertrophy in the late-gestation fetal sheep; whether these effects are sustained is unknown. IGF-I was infused for 4 days at 80 microg/h from 121 to 125 days of gestation, and its effects at 128 days, 3 days after the infusion stopped, were determined by comparison with untreated fetal sheep. After IGF-I treatment, fetal weights were similar to those in control fetuses but kidney weights were bigger (P < 0.05), as were spleen weights of male fetu… Show more

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Cited by 26 publications
(24 citation statements)
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“…It is well established that insulin-like growth factors also play a key role in regulating cardiomyocyte growth during cardiac development (9,26,41), and recent studies in ovine cardiomyocytes suggest that the IGFs, in particular IGF-2, play a role in the induction of cardiac hypertrophy in offspring exposed to fetal growth restriction (44). In support of these findings, we observed a marked increase in Igf1 and Igf2 gene expression at gestational day 20 as a result of growth restriction, with a concomitant increase in cardiac Cmyc mRNA expression.…”
Section: Discussionmentioning
confidence: 99%
“…It is well established that insulin-like growth factors also play a key role in regulating cardiomyocyte growth during cardiac development (9,26,41), and recent studies in ovine cardiomyocytes suggest that the IGFs, in particular IGF-2, play a role in the induction of cardiac hypertrophy in offspring exposed to fetal growth restriction (44). In support of these findings, we observed a marked increase in Igf1 and Igf2 gene expression at gestational day 20 as a result of growth restriction, with a concomitant increase in cardiac Cmyc mRNA expression.…”
Section: Discussionmentioning
confidence: 99%
“…Fresh piglet cardiac myocytes were isolated using methods described previously with minor modifications [9], [28]. Briefly, excised hearts were weighed and the free walls of the left and right ventricles, the atria, and the interventricular septum were dissected and individually weighed.…”
Section: Methodsmentioning
confidence: 99%
“…Cardiac myocyte nucleation and volume were determined as previously described [9], [27], [28]. Damaged or super-contracted cells were excluded.…”
Section: Methodsmentioning
confidence: 99%
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“…We obtained cardiomyocytes from CBP 48-h hearts (n=5), as described previously [31,32]. Briefly, a combination of enzyme digestion and mechanical dispersion was used to isolate cardiac ventricular myocytes from CBP 48-h hearts.…”
Section: Methodsmentioning
confidence: 99%