Effects of intraocular perfusion with two alternating irrigation solutions on the simultaneously recorded electroretinogram of albino rabbits

Textorius, Ola; Nilsson, Sven Erik G.; Andersson, Björn-Erik

doi:10.1007/bf00220226

Cited by 15 publications

(8 citation statements)

References 20 publications

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“…The composition of the intraocular irrigation solution PHS was recently discussed in detail [18]. It was found that PHS in many respects satisfied the demands of the retina in a better way than the more commonly used balanced salt solution (BSS).…”

Section: Discussionmentioning

confidence: 99%

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Effects of intravitreal perfusion with dopamine in different concentrations on the DC electroretinogram and the standing potential of the albino rabbit eye

1989

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The direct current electroretinogram and the standing potential were recorded from both eyes of 23 albino rabbits during intraocular perfusion of one of the eyes, which was vitrectomized, with a physiologic reference solution (PHS). PHS was then replaced by a test solution containing dopamine dissolved in PHS. The fluids were subsequently alternated (PHS-dopamine-PHS). During irrigation with 0.25-0.5 mM dopamine (11 rabbits) the c-wave amplitude was 140% higher (p less than 0.001) and during irrigation with 25 mM dopamine (6 rabbits) 85% lower (p less than 0.01) than it was during the corresponding initial perfusion with PHS. The simultaneously recorded b-wave amplitude was reduced (0.25-0.5 mM: -22%, p less than 0.001; 25 mM: -69%, p less than 0.001) and the SP level increased (0.25-0.5 mM: +2375 microV, p less than 0.01; 25 mM: +2530 microV, p less than 0.05) compared with the values obtained during the corresponding preceding irrigation with PHS. Thus the changes in the b- and c-wave amplitudes during perfusion with dopamine were dependent on the concentration of the drug. In the contralateral control eye (23 rabbits) the c-wave amplitude was 21% higher (p less than 0.001), the b-wave amplitude 14% higher (p less than 0.001) and the standing potential 1007 microV higher (p less than 0.001) during intravitreal perfusion with dopamine in the other eye than during the preceding irrigation with PHS in that eye, possibly as a result of increasing dark adaptation.

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Section: Discussionmentioning

confidence: 99%

“…The technique used in the present experiments was developed by Textorius and associates [18,19], and is briefly described here.…”

Section: Methodsmentioning

confidence: 99%

Effects of intravitreal perfusion with dopamine in different concentrations on the DC electroretinogram and the standing potential of the albino rabbit eye

1989

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“…The experimental model used in this study for exposure of the retina to different pharmacological substances was more fully described earlier [2,8]. Albino rabbits weighing 2.8-4.3 kg were studied under general anesthesia (pentobarbital).…”

Section: Methodsmentioning

confidence: 99%

Influence of isobutylmethylxanthine on the direct current electroretinogram of albino rabbit eyes

Jarkman

1989

Doc Ophthalmol

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The direct current electroretinogram and the standing potential of the eye were recorded from both eyes of unilaterally vitrectomized albino rabbits. The effect of intraocular irrigation with the nonselective phosphodiesterase inhibitor isobutylmethylxanthine (IBMX) was compared with that of a balanced salt control solution. IBMX (0.5 mM) produced a reversible increase in the amplitudes of the a-wave (19%, p less than 0.02), b-wave (21%, p less than 0.001) and c-wave (12%, p less than 0.01) of the electroretinogram. A small elevation of the standing potential was found (0.4 +/- 0.2 mV), but this increase was not statistically significant (p greater than 0.05). The results indicate a primary and principal influence of IBMX on the photoreceptors.

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“…A procedure for the administration of substances to the eye after vitrectomy, developed at our laboratory, was used (Textorius et al, 1986). The head of the animal was stabilized with a special head support.…”

Section: Methodsmentioning

confidence: 99%

Effects of low doses of forskolin on the c-wave of the direct current electroretinogram and on the standing potential of the eye

Jarkman

1987

Doc Ophthalmol

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A marked effect of prostaglandins on the b- and c-waves of the direct current electroretinogram was recently reported by our laboratory. The increased b- and c-wave amplitudes in response to prostaglandins may be mediated by cyclic nucleotides acting on fluid and ion transport across the retinal pigment epithelium (RPE). Forskolin is known to increase cyclic adenosine monophosphate in a number of tissues, among them the RPE. To study possible effects of forskolin on the ERG vitrectomy was performed on rabbit eyes, followed by intraocular irrigation with a forskolin solution (10 micrograms/ml PHS). Forskolin produced reversible ERG changes with increase in a- (24%, p less than 0.001), b- (25%, p less than 0.001) and c-wave (53%, p less than 0.001) amplitudes and elevation (about 1.0-1.5 mV, p less than 0.01) of the standing potential of the eye. The increase in c-wave amplitude was significantly greater than that of the a- (p less than 0.05) and b- (p less than 0.01) wave amplitudes, which seems to imply a primary or at least major effect on the RPE.

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Effects of intraocular perfusion with two alternating irrigation solutions on the simultaneously recorded electroretinogram of albino rabbits

Cited by 15 publications

References 20 publications

Effects of intravitreal perfusion with dopamine in different concentrations on the DC electroretinogram and the standing potential of the albino rabbit eye

Effects of intravitreal perfusion with dopamine in different concentrations on the DC electroretinogram and the standing potential of the albino rabbit eye

Influence of isobutylmethylxanthine on the direct current electroretinogram of albino rabbit eyes

Effects of low doses of forskolin on the c-wave of the direct current electroretinogram and on the standing potential of the eye

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