Effects of Ion Beams Pretreatment on Damage of UV-B Radiation on Seedlings of Winter Wheat (Triticum aestivum L.)

Abstract

The seeds of winter wheat were pretreated with three different doses of low-energy N(+) beams, and its seedlings were subjected to UV-B irradiation (10.08 kJ m(-2) day(-1)) at three-leaves stage. The growth characteristic of seeds, the oxidative damage to membrane system induced by UV-B radiation, and the alleviating effects of N(+) beams pretreatment to radiation damage were investigated. The results showed that the germination rate and seedling rate, respectively, increased 14.09 ± 1.03 and 13.91 ± 1.21 % compared with control (CK) at the dose of 4.0 × 10(16) ions/cm(2). When seedlings were exposed to UV-B radiation, the pretreatment method under the dose of 4.0 × 10(16) ions/cm(2) made the activity of peroxidase and superoxide dismutase increasing, the content of chlorophyll enhancing, but the content of malondialdehyde reducing significantly compared with that of the single UV-B radiation. Whereas, the activity of catalase irradiated by UV-B improved notably under the pretreatment dose of 8.0 × 10(16) ions/cm(2). In addition, after being irradiated with UV-B, the content of soluble protein and glutathione whose seeds were pretreated by the dose of 6.0 × 10(16) ions/cm(2) were higher than that of the single UV-B radiation. It was suggested that the suitable dose of low-energy ion beams pretreatment to wheat seeds could change its physiological characteristics at seedlings stage to alleviate the damage effects from UV-B radiation.

A Systematic Investigation of Key Factors of Nucleic Acid Precipitation Toward Optimized DNA/RNA Isolation

“…All of the recovery rates were analyzed via least significant difference pairwise comparison methods using SPSS software version 13.0 (IBM Corporation, NY, USA). The recoveries which have no significant difference with each others were marked with same letter [18][19][20]. Besides, all RNA/DNA solutions were adjusted to a relatively low level (100 ng/μl) prior to precipitation procedures, owing to the fact that nucleic acids of high initial concentrations normally achieve a higher recovery rate compared with those of lower initial concentrations [5], which may obscure the distinctive effects of various conditions of each influence factor on nucleic acid recovery levels.…”

Cadmium Telluride Quantum Dots (CdTe-QDs) and Enhanced Ultraviolet-B (UV-B) Radiation Trigger Antioxidant Enzyme Metabolism and Programmed Cell Death in Wheat Seedlings

“…Soluble protein was extracted according to Zhao [29] , using bovine serum albumin as a calibration standard. Fresh tissues (1.0 g) were ground in 6 mL distilled water and then centrifuged at 4000 rpm for 15 min.…”

Ionizing Radiation: Effective Physical Agents for Economic Crop Seed Priming and the Underlying Physiological Mechanisms

“…For instance, in Dolichos melanophthalmus , two doses (1 and 10 Gy) of a carbon and titanium ion treatment increased the contents of starch, H 2 O 2 , and AsA compared with those of the control [ 95 ]. A low-energy nitrogen ion beam (4.0 × 10 16 ions/cm 2 ) treatment at the pre-sowing stage also has had a stimulation impact on the content of soluble protein and GSH and the activities of CAT in wheat seedlings [ 96 ]. So, it is not difficult to conclude that seed priming with a low-dose heavy-ion beam could be considered to be a beneficial application in agricultural production.…”