Effects of ionized alkali water intake on oxidative stress and its related gene expression induced by acute and exhaustive exercise.

Abstract

Kim, B. R., Im, I. S. Effects of ionized alkali water intake on oxidative stress and its related gene expression induced by acute and exhaustive exercise. Exercise Science, 21(1): 1-10, 2012. The purpose of this study was to investigate the effects of ionized alkali water supplement on oxidative stress and proinflammatory cytokines in highly trained high school and college track athletes (n=18). In an single group and cross-over design, all subjects consumed tap water and ionized alkali water for 2 weeks in a random order, and they underwent an acute and exhaustive treadmill running at an intensity of 80% VO2max. Venous blood samples were collected prior to, immediately after, and 2 hr and 24 hr of post-exercise period for the assessment of serum MDA, TNF , and IL-6. In addition, relA α (p65) protein, iNOS mRNA, COX2 mRNA, and IL-6 mRNA were measured in peripheral blood mononuclear cells (PBMC). Two-way mixed ANOVA was applied to determine any significant treatment by time interaction in the measured variables, which was followed by independent t-test for post-hoc analyses if necessary. From the current study, we found that 2 weeks of ionized alkali water supplement suppressed increases in serum MDA, PBMC relA (p65) protein, and PBMC COX2 mRNA expression induced by the acute and exhaustive treadmill exercise in this study population. The findings of the study suggest that ionized alkali water supplement can result in somewhat anti-oxidant effects against free radical and reactive oxygen species occurring during a high intensity and exhaustive exercise.