Effects of ionizing radiation on the early development of Oryzias eggs.

Suyama, Ippei; Etoh, Hisami; Maruyama, Takashi; Kato, Yoshiya; Ichikawa, Ryushi

doi:10.1269/jrr.22.125

Cited by 23 publications

(10 citation statements)

References 3 publications

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“…Being a soft b-emitter, 3 H is generally regarded as being less biologically harmful than c-or X-rays. However, it appears that the relative biological effectiveness (RBE) for b radiation could be greater for some aquatic invertebrates (Knowles and Greenwood, 1997;Suyama et al, 1981). It has been suggested that enhanced RBE of 3 H is due to the fact that it induces 10-30 times greater ionisation per unit of tissue volume compared with X-or c-rays (Moskalev et al, 1973).…”

mentioning

confidence: 99%

Are low doses of tritium genotoxic to Mytilus edulis?

Jha

Dogra

Turner

et al. 2006

Marine Environmental Research

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mentioning

confidence: 99%

Are low doses of tritium genotoxic to Mytilus edulis?

Jha

Dogra

Turner

et al. 2006

Marine Environmental Research

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“…Doses utilized (0.1, 0.5, 1, 2 and 5 Gy) were selected on the basis of X-ray ranges used on different cell types (Suyama et al 1981;Antoccia et al 2002;Lanza et al 2005;Al-Jahdari et al 2008). The route of exposure (indirect radiation bursts given to cultures, as opposed to radionuclides containing sea water) and doses used were also motivated by the possibility to use the sea urchin embryo as a model organism in comparative risk assessment studies performed in the frame of National Space Agency program (Ambesi Impiombato et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Embryonic development and skeletogenic gene expression affected by X-rays in the Mediterranean sea urchin Paracentrotus lividus

et al. 2009

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International concern over environmental nuclear contamination of salt water fisheries and coastal resources has attracted the interests of ecologists, marine biologists and stakeholders. There are not many studies on the effects of X-rays, a component of radionuclides emissions, on embryonic development and gene expression. The sea urchin embryo is emerging as a useful model system for environmental and eco-toxicological studies. Here, we describe how X-rays affect development and gene expression in embryos of the Mediterranean sea urchin Paracentrotus lividus. Cleavage embryos were exposed to doses from 0.1 to 5 Gy, using an Ag source of X radiation. We found a dose-dependent increase in developmental delays and severe morphological defects in embryos microscopically inspected at two endpoints, 24 and 48 h after irradiation. By analogy with classical toxicity tests parameters we defined the No Observed Effect Dose at 0.1 Gy, the Lowest Observed Effect Dose at 0.5 Gy and ED50 at 1.0 Gy. Major perturbations concerned primitive intestine and skeleton differentiation and development: X-rays exposed embryos had both no gut and arms or poorly and abnormally developed ones. We found a dose-dependent reduction in the mRNA levels of two skeleton-specific genes, Pl-SM30 (spicule matrix 30) and Pl-msp130 (matrix spicule protein 130), as measured by semi-quantitative RT-PCR and whole mount in situ hybridization, respectively. These findings indicate the sea urchin embryo as a sensible bioindicator of X-radiation and propose its use as an alternative model, emphasizing the need for further investigation aimed to protect ecosystem health.

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“…This result could indicate that repair mechanisms are induced after 4 dpf in response to HTO. Other studies have shown that tritium can induce genotoxicity, with an increase of chromosomal aberrations in medaka eggs exposed to 2.08 × 10 1 µGy/h (Suyama et al ) and of DNA damage and micronuclei frequency at 1.25 × 10 1 µGy/h in mussels (Jha et al ). Tritiated thymidine also induced an increase of DNA damage in 4‐dpf larvae exposed at 1.05 × 10 4 µGy/h (Adam‐Guillermin et al ).…”

Section: Discussionmentioning

confidence: 97%

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

Gagnaire

Arcanjo

Cavalié

et al. 2020

Enviro Toxic and Chemistry

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Tritium, a radioactive isotope of hydrogen of natural and anthropogenic origin, is ubiquitously present in the environment. Effluents of nuclear centers of production are significant anthropogenic sources. With the upcoming project of thermonuclear fusion, tritium releases in the environment may increase. It is therefore important to characterize the ecological risk linked to tritium. The effects of tritiated water (HTO) were therefore studied in zebrafish larvae exposed for 10 d to different dose rates, 1.1 × 102, 4.1 × 102, and 3.8 × 103 µGy/h for larvae corresponding, respectively, to a water contamination of 104, 105, and 106 Bq/mL of HTO. Those dose rates were higher than 10 μGy/h, which is the threshold recommended to start monitoring ecosystems where radiological contaminants are present. Mortality, embryo‐larval development, immune toxicity, genotoxicity, neurotoxicity, and alterations of tissues were investigated. The results showed that HTO exposure induced DNA damage and reactive oxygen species production and modulated the expression of genes involved in detoxification processes. Moreover, modifications of the muscular tissues (degradation of myofibrils at 4 d post fertilization and disorganization of mitochondria at later stages) were observed. The results differed with HTO dose rates and with developmental stages. These results will drive future research for the development of new HTO‐sensitive biomarkers and will allow us to progress in the characterization of the modes of action of tritium in fish. Environ Toxicol Chem 2020;39:648–658. © 2019 SETAC

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Effects of ionizing radiation on the early development of Oryzias eggs.

Cited by 23 publications

References 3 publications

Are low doses of tritium genotoxic to Mytilus edulis?

Are low doses of tritium genotoxic to Mytilus edulis?

Embryonic development and skeletogenic gene expression affected by X-rays in the Mediterranean sea urchin Paracentrotus lividus

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

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