Effects of iron on rainbow trout gill cells in primary culture

Leguen, Isabelle; Perón, Sandrine; Prunet, Patrick

doi:10.1007/s10565-011-9189-3

Cited by 5 publications

(2 citation statements)

References 36 publications

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“…Within the intestinal epithelium, monolayer cells possess dual functions, as they both absorb essential substances and serve as a defense against harmful ones. The luminal cells in the intestine are closely linked to the epithelial cells by a brush border, forming a relatively impermeable membrane [95]. These intestinal epithelial cells also act as vital barriers to protect the gut and can be influenced by dietary nutrition and environmental factors [96].…”

Section: Fish Intestinal Epithelial Cellsmentioning

confidence: 99%

Profiling the Physiological Roles in Fish Primary Cell Culture

He,

Zhao,

Xiao

et al. 2023

Biology

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Fish primary cell culture has emerged as a valuable tool for investigating the physiological roles and responses of various cell types found in fish species. This review aims to provide an overview of the advancements and applications of fish primary cell culture techniques, focusing on the profiling of physiological roles exhibited by fish cells in vitro. Fish primary cell culture involves the isolation and cultivation of cells directly derived from fish tissues, maintaining their functional characteristics and enabling researchers to study their behavior and responses under controlled conditions. Over the years, significant progress has been made in optimizing the culture conditions, establishing standardized protocols, and improving the characterization techniques for fish primary cell cultures. The review highlights the diverse cell types that have been successfully cultured from different fish species, including gonad cells, pituitary cells, muscle cells, hepatocytes, kidney and immune cells, adipocyte cells and myeloid cells, brain cells, primary fin cells, gill cells, and other cells. Each cell type exhibits distinct physiological functions, contributing to vital processes such as metabolism, tissue regeneration, immune response, and toxin metabolism. Furthermore, this paper explores the pivotal role of fish primary cell culture in elucidating the mechanisms underlying various physiological processes. Researchers have utilized fish primary cell cultures to study the effects of environmental factors, toxins, pathogens, and pharmaceutical compounds on cellular functions, providing valuable insights into fish health, disease pathogenesis, and drug development. The paper also discusses the application of fish primary cell cultures in aquaculture research, particularly in investigating fish growth, nutrition, reproduction, and stress responses. By mimicking the in vivo conditions in vitro, primary cell culture has proven instrumental in identifying key factors influencing fish health and performance, thereby contributing to the development of sustainable aquaculture practices.

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Section: Fish Intestinal Epithelial Cellsmentioning

confidence: 99%

Profiling the Physiological Roles in Fish Primary Cell Culture

He,

Zhao,

Xiao

et al. 2023

Biology

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“…Rainbow trout -SSI Effect of cortisol on occludin expression and permeability Sandbichler et al, 2011a Rainbow trout -SSI Morphology and effect of cortisol and membrane permeability Sandbichler et al, 2011b Rainbow trout -SSI Claudin 28b expression: osmotic stress and cortisol Farkas et al, 2011 Rainbow trout -DSI Silver nanoparticle uptake and cytotoxicity Leguen et al, 2011 Rainbow trout -wells Iron toxicity Chasiotis and Kelly, 2011a Rainbow trout and goldfish -SSI Cortisol effect on permeability, claudin and ZO-1 expression Chasiotis and Kelly, 2011b Goldfish -SSI Occludin expression and membrane permeability Chow and Wong, 2011…”

Section: The Primary Gill Cell Culturementioning

confidence: 99%

Gill cell culture systems as models for aquatic environmental monitoring

Bury

Schnell

Högstrand

2014

Journal of Experimental Biology

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A vast number of chemicals require environmental safety assessments for market authorisation. To ensure acceptable water quality, effluents and natural waters are monitored for their potential harmful effects. Tests for market authorisation and environmental monitoring usually involve the use of large numbers of organisms and, for ethical, cost and logistic reasons, there is a drive to develop alternative methods that can predict toxicity to fish without the need to expose any animals. There is therefore a great interest in the potential to use cultured fish cells in chemical toxicity testing. This review summarises the advances made in the area and focuses in particular on a system of cultured fish gill cells grown into an epithelium that permits direct treatment with water samples. KEY WORDS: FIGCS, Biomonitoring, Environmental risk assessment, Fish, In vitro, Toxicology IntroductionThe Industrial Revolution caused a rapid rise in the use of raw materials and urbanisation as the populace moved to the cities for employment. Since this time, there has been a continuous increase in living standards that to a large part has been fuelled by innovations within the chemical and pharmaceutical industry. Life expectancy has increased as a result of great advances in medical practices and effective drugs against many fatal diseases. The increase in life expectancy has seen the population of the world grow, reaching 7 billion in 2012, and to feed this population there have been great advances in agricultural productivity partly via the development of pesticides and nitrate/phosphate-based fertilisers. These activities have altered the geochemical cycling of elements, increasing or decreasing concentrations in earth system compartments and increasing global distribution (Doney, 2010). Anthropogenic activities have left a cumulative and lasting impression on the biosphere -so much so that geologists have termed the current epoch the anthropocene (Zalasiewicz et al., 2010).The increase in agricultural and industrial production and consumption of raw materials has resulted in the creation of vast amounts of waste that enters the aquatic ecosystem. An acknowledgement of the decline in environmental quality due to contaminants has led to the development of environmental quality standards (EQS) in many countries, and to assess whether these standards are being adhered to, many jurisdictions also have a programme of waste water effluent testing (WET) and/or biomonitoring. The EQS are derived from toxicity tests that use numerous organisms per compound, and in order to set standards several species are tested. In the USA ~3 million fish are used in WET procedures (see Tanneberger et al., 2013). There is a move towards reducing the number of animals used in research and toxicology studies and there are a number of international initiatives aimed at investigating the 3Rs -reduction, replacement and refinement -in animal research (for example, see http://www.nc3rs.org.uk/). Within the context of the need to determine EQS fo...

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