Effects of Keratinocyte Growth Factor on the Uterine Endometrial Epithelial Cells in Pigs

Ka, Hakhyun; Bazer, Fuller W.

doi:10.5713/ajas.2005.1708

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…FGF7 is expressed in the endometrial epithelia of pigs; its receptor, FGFR2, is present in both the endometrial epithelia and conceptus Tr, and FGF7 stimulates the Tr, but not endometrial epithelial cells, to undergo proliferation and differentiation. Therefore, FGF7 is proposed to be a paracrine mediator of interactions between the uterus and the conceptus (Ka et al 2000, Ka & Bazer 2005). FGF7 mRNA is induced in LE between days 12 and 15 of the estrous cycle and pregnancy, but GE expression is delayed until days 30 and 40 of pregnancy (Ka et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium

et al. 2010

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Pigs experience significant conceptus loss near mid-gestation, correlating with increasing glandular epithelial (GE) development and secretory activity. Secreted phosphoprotein 1 (SPP1, osteopontin) increases in GE between days 30 and 40 of pregnancy and is expressed in the GE of day 90 pseudopregnant pigs, suggesting that progesterone (P 4 ) from corpora lutea is responsible for induction of SPP1 in GE. In this study, pigs were ovariectomized and treated daily with P 4 to assess effects of 40 days of P 4 exposure on SPP1, P 4 receptor (PGR), uteroferrin (ACP5), and fibroblast growth factor 7 (FGF7) expression in porcine endometria. PGR mRNA decreased in pigs injected with P 4 compared with pigs injected with corn oil (CO), and PGRs were downregulated in the luminal epithelium (LE) and GE. ACP5 mRNA increased in pigs injected with P 4 compared with pigs injected with CO, and ACP5 was induced in the GE of P 4 -treated pigs. FGF7 mRNA increased in pigs injected with P 4 compared with pigs injected with CO, and FGF7 was induced in the LE and GE of P 4 -treated pigs. SPP1 mRNA was not different between pigs injected with P 4 compared with pigs injected with CO, and SPP1 was not present in the GE of P 4 -treated pigs. Therefore, long-term P 4 , in the absence of ovarian and/or conceptus factors, does not induce SPP1 expression in GE. We hypothesize that a servomechanism involving sequential effects of multiple hormones and cytokines, similar to those for sheep and humans, is required for GE differentiation and function, including the synthesis and secretion of SPP1.

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Section: Discussionmentioning

confidence: 99%

Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium

et al. 2010

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“…In the different mammalian species such as mouse (Moreno et al, 2001), guinea pig (Huang et al, 1982), hamster (Ahuja, 1985;Moreno et al, 2001), rat (Shalgi et al, 1986) and human (Huang et al, 1982;Mahony et al, 1991;Oehninger et al, 1992), the sperm-zona binding is strongly inhibited by polysaccharide fucoidan, an algal sulfated L-fucose polymer. Thus, fucose seemed to be part of the recognition signal of gamete interaction in mammals (Huang et al, 1982;Huang and Yanagimachi, 1984;Ka and Bazer, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effect of Monosaccharide L-fucose and Polysaccharide Fucoidan on Sperm ??L-fucosidase Activity and Relation to Sperm-oocyte Interaction in Pig

Song¹,

Park²,

Piao³

et al. 2007

Asian Australas. J. Anim. Sci

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Carbohydrate-protein interactions are known to be important in gamete interactions. Several evidence indicated that a fucose-containing sulfated polysaccharide fucoidan was potential inhibitor of fertilization in vitro and thus fucose seemed to be part of the recognition signal of gamete interaction in mammals. In recent investigation we found that α-L-fucosidase activity was present in boar spermatozoa and it was related to sperm binding to and penetration into zona pellucida (ZP) in vitro. The objective of this study was to determine the effects of monosaccharide L-fucose and polysaccharide fucoidan on sperm α-L-fucosidase activity and relation to sperm-oocyte interaction in pig. Results indicated that the activity of sperm α-L-fucosidase was largely inhibited (62%) when sperm suspension was treated with monosaccharide L-fucose. It also significantly inhibited the number of sperm binding to ZP (32%) and penetration into zona-intact oocytes (72%), but did not inhibit penetration into zona-free oocytes when fertilization medium contained Lfucose. The chlorotetracycline (CTC) assessment showed that L-fucose did not affect induction of sperm capacitation and acrosome reaction. In contrast, the activity of sperm α-L-fucosidase was not inhibited when sperm suspension was treated with polysaccharide fucoidan but sperm-ZP binding was greatly inhibited (85%) and completely blocked sperm penetration into zona-intact or zona-free oocytes. The CTC assessment showed that fucoidan increased the F pattern and decreased the AR pattern sperm. These results suggested that the different inhibitory mechanisms were present between monosaccharide L-fucose and polysaccharide fucoidan on sperm-oocyte interaction, the inhibition effect of α-L-fucose on sperm binding and penetrating into ZP caused sperm α-L-fucosidase inhibited by α-Lfucose.

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Regulation of Expression of Fibroblast Growth Factor 7 in the Pig Uterus by Progesterone and Estradiol1

Al-Ramadan

Erikson

et al. 2007

Biology of Reproduction

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Fibroblast growth factor 7 (FGF7) stimulates cell proliferation, differentiation, migration and angiogenesis. The consensus is that FGF7, expressed by mesenchymal cells, binds FGF receptor 2IIIb (FGFR2) on epithelia, thereby mediating epithelial-mesenchymal interactions. The pig uterus is unique in that FGF7 is expressed by the luminal epithelium (LE) and FGFR2 is expressed by the LE, glandular epithelium (GE), and trophectoderm to effect proliferation and differentiated cell functions during conceptus development and implantation. FGF7 expression by the uterine LE of pigs increases between Days 9 and 12 of the estrus cycle and pregnancy, as circulating concentrations of progesterone increase, progesterone receptors (PGR) in the uterine epithelia decrease, and the conceptuses secrete estradiol-17beta (E(2)), for pregnancy recognition. Furthermore, E(2) increases the expression of FGF7 in pig uterine explants. The present study investigates the relationships between progesterone, E(2), and their receptors and the expression of FGF7 in the pig uterus in vivo. Pigs were ovariectomized on Day 4 of the estrus cycle and injected i.m. daily from Day 4 to Day 12 with either corn oil (CO), progesterone (P4), P4 and ZK317,316 (PZK), E(2), P4 and E(2) (PE), or P4 and ZK and E(2) (PZKE). All gilts (n = 5/treatment) were hysterectomized on Day 12. The results suggest that: 1) P4 is permissive to FGF7 expression by down-regulating PGR in LE; 2) P4 stimulates PGR-positive uterine stromal cells to release an unidentified progestamedin that induces FGF7 expression by LE; 3) E(2) and P4 can induce FGF7 when PGR are rendered nonfunctional by ZK; and 4) E(2) from conceptuses interacts via estrogen receptor alpha, but not estrogen receptor beta in LE to induce maximal expression of FGF7 in LE on Day 12 of pregnancy in pigs.

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Effects of Keratinocyte Growth Factor on the Uterine Endometrial Epithelial Cells in Pigs

Cited by 5 publications

References 24 publications

Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium

Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium

Effect of Monosaccharide L-fucose and Polysaccharide Fucoidan on Sperm ??L-fucosidase Activity and Relation to Sperm-oocyte Interaction in Pig

Regulation of Expression of Fibroblast Growth Factor 7 in the Pig Uterus by Progesterone and Estradiol1

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