Effects of L-Serine Against Cisplatin-Mediated Reactive Oxygen Species Generation in Zebrafish Vestibular Tissue Culture and HEI-OC1 Auditory Hybridoma Cells

Monroe, Jerry D.; Moolani, Satya A.; Irihamye, Elvin N.; Johnston, Alexandra M.; Smith, Michael E.

doi:10.1007/s12640-020-00188-y

Cited by 11 publications

(13 citation statements)

References 24 publications

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“… 17 , 26 , 27 , 40 In one experimental protocol using adult zebrafish, a single microinjection of cisplatin and drug was applied, whereas when cell cultures were used, the experimental procedure included incubation with cisplatin and then co-incubation with the otoprotective substances. 33 , 35 , 36 …”

Section: Resultsmentioning

confidence: 99%

“… 6 , 32 In another two studies, in which the inner ear cell of zebrafish was cultured, spectrophotometry was used. 33 , 35 Lastly, one publication assessed the hearing abilities of adult zebrafish by measuring auditory brainstem responses (ABR). 36 …”

Section: Resultsmentioning

confidence: 99%

“…[17][18][19][20][21][22][23][24][25][26][27][28] The second EM was compsed of 14.9 mM NaCl, 0.503 mM KCl, 0.994 mM MgSO 4 , 0.150 mM KH 2 PO 4 , 42 mM Na 2 HPO 4 , 0.986 mM CaCl 2 , 0.714 mM NaHCO 3 in distilled water. 12,[29][30][31]32,33 In addition, the embryos were also kept in E3 embryo media consisting of 5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl 2 , 0.33 MgSO 4 or in a standard bath solution containing: 120 mM NaCl, 2 mM KCl, 10 mM HEPES (2-[4-(2-hydroxyethyl) piperazin-1-yl]ethanesulfonic acid), 2 mM CaCl2 and 0.7 mM NaH2PO4 adjusted to pH 7.2. 21,34 For the anesthesia, MS-222 (3-aminobenzoic acid ethyl ester, methanesulfonate salt) was used.…”

Section: Zebrafish Culture Conditionsmentioning

confidence: 99%

“…17,20,23,26 Similarly, the EF-24 was suggested to prevent intracellular ROS formation via inhibition of NF-kB-induced signaling and suppressed expression of oncogenic miR-NAs, including miR-21. 33 Also dimethyl sulfoxide (DMSO) used at low concentrations is a known scavenger of the hydroxyl radicals. 41 Apoptosis is a programmed cell death initiated via the intrinsic or extrinsic pathway.…”

Section: Mechanism Of Otoprotective Action Of Studied Compounds and Mmentioning

confidence: 99%

“…In the majority of the reviewed studies, the assessment of the otoprotective and ototoxic effects involved sensory hair cell counting (eighteen articles). 12,17,18,[20][21][22]24,25,27,28,30,31,[32][33][34]39,40 An additional method, evaluating the hair cell function, was the uptake of FM1-43 dye (four articles) and the recording of microphonic potentials (two articles). 19,21,25,34,43 In six articles, the TUNEL assay was applied, detecting single-stranded brakes in the chromosomal DNA (a feature of apoptosis).…”

Section: Outcome Measurementsmentioning

confidence: 99%

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Use of zebrafish larvae lateral line to study protection against cisplatin-induced ototoxicity: A scoping review

Domarecka

Skarzynska

Szczepek

et al. 2020

Int J Immunopathol Pharmacol

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Aim: The present review aimed to consolidate and analyze the recent information about the use of zebrafish in studies concerning cisplatin-induced ototoxicity and otoprotection. Material and methods: The PubMed, Web of Science, and Scopus databanks were searched using the following MESH terms: zebrafish, cisplatin, ototoxicity. The identified publications were screened according to inclusion and exclusion criteria and the 26 qualifying manuscripts were included in the full-text analysis. The experimental protocols, including cisplatin concentrations, the exposure duration and the outcome measurements used in zebrafish larvae studies, were evaluated and the reported knowledge was summarized. Results: Twenty-six substances protecting from cisplatin-induced toxicity were identified with the use of zebrafish larvae. These substances include quinine, salvianolic acid B, berbamine 6, benzamil, quercetin, dexmedetomidine, dexamethsanone, quinoxaline, edaravone, apocynin, dimethyl sulfoxide, KR-22335, SRT1720, ORC-13661, 3-MA, D-methionine, mdivi-1, FUT-175, rapamycin, Z-LLF-CHO, ATX, NAC, CYM-5478, CHCP1, CHCP2 and leupeptin. The otoprotective effects of compounds were attributed to their anti-ROS, anti-apoptotic and cisplatin uptake-blocking properties. The broadest range of protection was achieved when the experimental flow used preconditioning with an otoprotective compound and later a co-incubation with cisplatin. Protection against a high concentration of cisplatin was observed only in protocols using short exposure times (4 and 6 h). Conclusions: The data extracted from the selected papers confirm that despite the differences between the human and the zebra fish hearing thresholds (as affected by cisplatin), the sensory cells of zebrafish and larval zebrafish are a valuable tool which could be used: (i) for the discovery of novel otoprotective substances and compounds; (ii) to screen their side effects and (iii) to extend the knowledge on the mechanisms of cisplatin-induced inner ear damage. For future studies, the development of a consensus experimental protocol is highly recommended.

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