Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on enterohemorrhagic Escherichia coli infection using mouse and intestinal cell models

Abstract

A potential probiotic strain, Lactobacillus kefiranofaciens M1, was previously isolated from kefir grains, which are used to manufacture the traditional fermented drink kefir. The aim of this study was to investigate the effects of Lb. kefiranofaciens M1 on enterohemorrhagic Escherichia coli (EHEC) infection, using mice and intestinal cell models. BALB/c mice were daily administrated with either phosphate buffered saline or Lb. kefiranofaciens M1 at 2×10(8) cfu/mouse per day intragastrically for 7 d. Intragastric challenges with EHEC (2×10(9) cfu/mouse) were conducted on d 0, 4, and 7 after treatment. Administration of Lb. kefiranofaciens M1 was able to prevent EHEC infection-induced symptoms, intestinal damage, renal damage, bacterial translocation, and Shiga toxin penetration. Furthermore, the mucosal EHEC-specific IgA responses were increased after Lb. kefiranofaciens M1 administration in the EHEC-infected mouse system. Additionally, in vitro, Lb. kefiranofaciens M1 was shown to have a protective effect on Caco-2 intestinal epithelial cells and Caco-2 intestinal epithelial cell monolayers; the bacteria limited EHEC-induced cell death and reduced the loss of epithelial integrity. These findings support the potential of Lb. kefiranofaciens M1 treatment as an approach to preventing EHEC infection and its effects.

Microbial Succession and Flavor Production in the Fermented Dairy Beverage Kefir

“…The presence of these genes suggests that the L. kefiranofaciens strains present in these kefirs have the potential to survive gastric transit, colonize the gut, and inhibit the growth of pathogens. Indeed, previous studies using mice have shown that L. kefiranofaciens protects against enterohemorrhagic Escherichia coli infection (44). Further analysis of shotgun metagenomic data from cheese and kimchi indicated that similar genes are present in other fermented foods.…”

The Microbiota and Health Promoting Characteristics of the Fermented Beverage Kefir

“…In an in vivo study where BALB/c mice were intragastrically challenged with E. coli O157:H7, mice receiving L . kefiranofaciens M1 prior to E. coli challenge showed reduced symptoms of infection, including intestinal and renal damage, bacterial translocation, and Shiga toxin penetration as well as increased EHEC-specific mucosal IgA responses ( Chen et al, 2013 )…”

Selecting probiotics with the abilities of enhancing GLP-1 to mitigate the progression of type 1 diabetes in vitro and in vivo

“…Previous studies have reported that administering probiotics to humans and animal models is beneficial in treating and preventing intestinal infections as well as reducing mucosal inflammation Chen, Hsiao, Hong, Dai, & Chen, 2012;Chen, Lee, Hong, Hsieh, & Chen, 2013). The ability of deviating tissue cytokine secretions from a proinflammatory to an antiinflammatory profile has been specifically described (Hong, Chen, & Chen, 2010;Shida, Kiyoshima-Shibata, Nagaoka, Watanabe, & Nanno, 2006;Shida et al, 2002).…”