Abstracts: Sugarcane has been widely used as a biofuel crop due to its high biological productivity, ease of conversion to ethanol, and its relatively high potential for greenhouse gas reduction and lower environmental impacts relative to other derived biofuels from traditional agronomic crops. In this investigation, we studied four sugarcane cultivars (H-65-7052, H-78-3567, H-86-3792 and H-87-4319) grown on a Hawaiian commercial sugarcane plantation to determine their ability to store and accumulate soil carbon (C) and nitrogen (N) across a 24-month growth cycle on contrasting soil types. The main study objective establish baseline parameters for biofuel production life cycle analyses; sub-objectives included (1) determining which of four main sugarcane cultivars sequestered the most soil C and (2) assessing how soil C sequestration varies among two common Hawaiian soil series (Pulehu-sandy clay loam and Molokai-clay). Soil samples were collected at 20 cm increments to depths of up to 120 cm using hand augers at the three main growth stages (tillering, grand growth, and maturity) from two experimental plots at to observe total carbon (TC), total nitrogen (TN), dissolved organic carbon (DOC) and nitrates (NO and H-87-4319 (20 kg·m −2 ) appeared to produce more accumulated carbon in both soil types.