In order to investigate the characteristics of soil structural stability and the factors influencing it under typical land use types in the karst region of western Hubei, soil samples were collected from five land use types (natural mixed forest [NF], cypress forest [CF], stone dike terrace [ST], stone dike forest [SF], and abandoned land [AL]) in Xialaoxi to analyze the particle size distribution of aggregates, structural stability, and the distribution characteristics of carbon, nitrogen, and extracellular enzyme contents. The results showed that the aggregates of NF, CF, SF, and AL were dominated by >2‐mm aggregates, while ST showed mostly 2–0.25‐mm aggregates. The mean weight diameter (EMWD) and the geometric mean diameter (EGMD) of mechanical aggregates showed a trend of CF > NF > SF > AL > ST, whereas the erodibility factor K showed an opposite trend. The total organic carbon (TOC), labile organic carbon (LOC), and total nitrogen (TN) contents of aggregates of each particle size were significantly higher in NF and CF than in ST and AL under the five land types. The content of available nitrogen (AN) was highest in NF and ST. The content of carbon and nitrogen and extracellular hydrolases (β‐glucosidase [BG], cellulose disaccharide hydrolase [CBH], β‐N‐acetyl‐aminoglucosidase [NAG], and leucine aminopeptidase [LAP]) in soil aggregates were mainly concentrated in <0.25–0.053‐mm aggregates. The variation in carbon and nitrogen abundance of each particle size agglomerate mainly originated from the LAP content of that particle size agglomerate and showed significant correlation. The partial least squares path models (PLS‐PM) showed that the main influences on the stability of soil aggregates in small watersheds were land use types, soil physical properties, carbon and nitrogen abundance, and extracellular enzyme activity. In conclusion, the structural stability of soil aggregates in Xialaoxi watershed is the result of the interaction between human disturbance and soil nutrient self‐cycling. In general, NF and CF have better soil aggregate stability and are conducive to soil carbon and nitrogen accumulation, whereas for later development and management of SF, ST, and AL sample plots, scientific cultivation means should be adopted, and attention should be paid to long‐term carbon and nitrogen accumulation and conservation.