Effects of lead on <i>Cladophora rupestris</i>: localization, subcellular distribution, and cell ultrastructure

Wang, Junjie; Chen, Qiu-yu; Liu, Lei; Liu, Yang; Zhang, Zhe; Zhang, Qian; Cao, De-ju

doi:10.1080/10889868.2021.1884039

Cited by 5 publications

(2 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some plants such as Rhus chinensis Mill ( Anacardiaceae ) and Conyza canadensis could overaccumulate heavy metals (HMs) [ 2 , 3 ]. Cladophora rupestris has a strong ability to absorb and tolerate Pb 2+ stress and alter the distribution of Pb at both the tissue and sub-cellular levels to reduce toxicity [ 4 , 5 ]. However, the tolerance and detoxification mechanisms of hyperaccumulate were complex [ 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential Gene Expression and Metabolic Pathway Analysis of Cladophora rupestris under Pb Stress Conditions

Liu

Zhang²,

Liu³

et al. 2022

IJERPH

Self Cite

View full text Add to dashboard Cite

This study aimed to analyze the transcriptome of C. rupestris under Pb2+ stress by using high-throughput sequencing technology, observe the changes of gene expression and metabolic pathway after three and five days under 1.0 and 5.0 mg/L of Pb2+ treatment, and analyze the differentially expressed genes (DEGs) and related functional genes after Pb2+ treatment. Metabolic pathways were revealed through Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results show that DEGs increased significantly with the increase of Pb2+ concentration and stress time. A total of 32 genes were closely related to Pb2+ stress response. GO analysis identified two major transporter proteins, namely, ATP-binding transport protein-related (ABC transporters) and zinc finger CCHC domain containing protein (Zfp) in C. rupestris. Pthr19248, pthr19211, Zfp pthr23002, Zfp p48znf pthr12681, Zfp 294 pthr12389, and Zfp pthr23067 played important roles against Pb2+ toxicity and its absorption in C. rupestris. KEGG pathway analysis suggested that ABCA1, ATM, and ABCD3 were closely related to Pb2+ absorption. Pb2+ stress was mainly involved in metallothionein (MT), plant hormone signal transduction, ABC transporters, and glutathione (GSH) metabolism.

show abstract

Section: Introductionmentioning

confidence: 99%

“…C. rupestris has some adsorptive ability and tolerance to Pb 2+ [ 5 ]. Based on our previous research, Cladophora could accumulate Pb 2+ but not Zn 2+ and Cu 2+ [ 4 , 6 ].…”

Section: Introductionmentioning

confidence: 99%