Effects of let‑7c on the proliferation of ovarian carcinoma cells by targeted regulation of CDC25a gene expression

Abstract

Abstract. MicroRNAs serve a role in the development of ovarian cancer (OC). The present study investigated whether let-7c is able to regulate the proliferation of OC cells by targeting cell division cycle 25A (CDC25a). The reverse transcription-quantitative polymerase chain reaction was performed to detect the expression of let-7c in OC specimens. Let-7c agomir was transfected into OC cells, and the proliferation and apoptosis of OC cells were detected. A dual-luciferase assay and western blotting were performed to analyze whether CDC25a was the target gene of let-7c as well as its interaction site. The results revealed that, in OC tissue, let-7c was downregulated when compared with normal ovarian tissue. A Cell Counting Kit-8 (CCK8) assay, colony formation assay and flow cytometry demonstrated that increased expression of let-7c was able to inhibit the proliferation and increase the apoptosis of OC cells. Western blotting revealed that upregulated let-7c is able to decrease the expression of CDC25a, and a dual-luciferase assay and a recovery assay demonstrated that let-7c was able to regulate the expression of the 3' untranslated region of CDC25a. Therefore, the roles of let-7c in inhibiting the proliferation and promoting the apoptosis of OC cells may be realized through the regulation of the expression of CDC25a. The results of the present study revealed that let-7c may be a novel target in the diagnosis and treatment of OC.

<p><em>Let-7c-5p</em> Inhibits Cell Proliferation and Migration and Promotes Apoptosis via the CTHRC1/AKT/ERK Pathway in Esophageal Squamous Cell Carcinoma</p>

“…It has been reported in a variety of solid tumors that let-7c-5p acts as a tumor suppressor, inhibiting cell proliferation, migration and invasion. [18][19][20][21][22][23] Although cluster analysis has found that let-7c is downregulated in ESCC tissues, the specific role and underlying mechanisms of let-7c in ESCC have not been further elucidated. To explore the mechanism of let-7c-5p in ESCC, first, we confirmed that let-7c-5p was clearly downregulated in ESCC tissues and five ESCC cell lines (TE-1, TE10, TE11, KYSE140 and KYSE150) compared to normal tissues and cells, which was consistent with the previously reported cluster analysis.…”

“…[15][16][17] The miRNA let-7, one of the earliest identified human miRNAs, participates in various biological processes such as cell proliferation, differentiation, apoptosis, hormone secretion, metabolism, immune regulation and tumorigenesis. Recent studies have found that let-7c-5p is significantly downregulated in a variety of human malignant tumors, including head and neck squamous cell carcinoma, 18 nonsmall cell lung cancer, 19 hepatocellular carcinoma, 20 colorectal cancer, 21 ovarian carcinoma 22 and glioma. 23 Importantly, based on the results of unsupervised hierarchical clustering in previous literature, it was found that let-7c was downregulated in ESCC tissues.…”