Effects of light and cytokinin on in vitro micropropagation and microrhizome production in turmeric (Curcuma longa L.)

Hashemy, Tafseera; Maki, Hisae; Yamada, Y.; Kaneko, Takako; Syōno, Kunihiko

doi:10.5511/plantbiotechnology.26.237

Cited by 9 publications

(9 citation statements)

References 16 publications

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“…Furthermore, the olfactive perception of the peculiar turmeric flavour was perceived after rhizome cutting. These results confirmed that an increase in sucrose concentration (6%) and a short-day photoperiod were necessary conditions for a good development of in vitro turmeric rhizomes [22,34]. Moreover the addition of charcoal represents a good tool for the establishment of the characteristic colour of these organs.…”

supporting

confidence: 70%

“…In vitro propagation method for selecting organs with quantified curcuminoids Natural Product Communications Vol. 7 (8) 2012 1039 In the literature turmeric microrhizomes were obtained in MS medium with a high sucrose concentration and a short photoperiod [33][34]. In this work the induction of microrhizomes was achieved by using the proposed short photoperiod to facilitate the storage phase of roots, and introducing the use of two different media, with a reduced agar percentage (0.5%) to facilitate microrhizome expansion.…”

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confidence: 99%

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Production of Curcuminoids in Different in vitro Organs of Curcuma longa

Pistelli

Bertoli

Gelli

et al. 2012

Natural Product Communications

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Curcuma longa L. (turmeric) is one of the most important spice and safe food additives. Its main constituents, curcuminoids, showed anti-inflammatory, antitumor and antioxidant effects. In the present work, an in vitro propagation method was developed to achieve selected plant organs with quantified curcuminoid content. In vitro plants were obtained from sprouting buds as primary explants. The major curcuminoid constituents, such as curcumin (CUR), demethoxycurcumin (DEM), and bis-demethoxycurcumin (bis-DEM) were examined in different organs by LC-DAD-ESI-MS. A significant production of curcumin (more than 260 µg g-1 fresh weight) was obtained from in vitro microrhizomes, especially grown in a Murashige and Skoog medium (MS) supplemented with kinetin (0.1 mg L-1), -naphthaleneacetic acid (NAA, 1 mg L-1), sucrose (6%), agar (5%) and activated charcoal (0.1%). The analyzed microrhizomes showed reduced amounts of DEM and bis-DEM in comparison with CUR levels. In addition a shoot culture line was suitable to biosynthesize curcuminoids, in a ratio very similar to that identified in the fresh rhizomes of parent plants. This study represents the first direct quantification of curcuminoids in turmeric in vitro shoots and microrhizomes to be used in dietary supplements.

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confidence: 70%

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confidence: 99%

Production of Curcuminoids in Different in vitro Organs of Curcuma longa

Pistelli

Bertoli

Gelli

et al. 2012

Natural Product Communications

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“…BAP alone or in combination with other growth regulators were found to have induced multiple shoots (Parthasarathy and Sasikumar, 2006). Hashemy et al, (2009) reported Kinetin at the concentration of 0.2 mg/L in the presence of 2 mg/L of NAA as the most favorable for the formation of shoots in Curcuma longa. The results obtained by Srirat et al, (2008) for the in vitro shoots propagation of Curcuma longa using rhizome buds as explants showed MS medium containing 60 g/L of sucrose and 5 mg/L of thidiazuron (TDZ) to have induced shoots.…”

Section: Resultsmentioning

confidence: 99%

“…Besides, a large amount of edible part (rhizome) is also stored for stock purpose for the next season planting, thereby-making germplasm maintenance by annual planting, an expensive and labor-intensive activity for the marginal farmers (Jala, 2013;Behera et al, 2010). Maximum multiplication achievable annually is only 5-fold (Hashemy, 2009). Shirgurkar et al, (2011) indicated the need for turmeric plant rapid multiplication technique.…”

Section: Introductionmentioning

confidence: 99%

Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

Abubakar

Pudake

2019

ASD

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Sterilization procedure, media composition, explants selection and control of physical environment are critical for successful cultures and callus induction with surface sterilization being very challenging in most plants. Five different sterilization methods were evaluated to come up with the best for subsequent use to establish an in vitro regeneration method for the induction of callus in Curcuma caesia using excised leaf and rhizome explants. Murashige and Skoog (MS) media supplemented with various concentration of 2,4-Dichlorophenoxy acetic acid (2,4-D)/Indole-3-acetic acid (IAA) (0.5- 5.0mg/L), singly or in combination with Benzyl aminopurine (BAP)/Kinetin (KIN) (0.1-5.0mg/L), 0.3% sucrose and 0.08% agar were used. The result of the sterilization procedures showed 15% NaHClO3 (5min) + 70% Ethanol (30s) + 0.1% HgCl2 (5min) to be the most effective in controlling contamination in C. caesia among all the treatments tested. The response to callus induction was found to depend on the type of explants used and growth regulators combination. Leaf explants gave the highest percentage of callus induction. Highest percentage of callus induction (66.70%) was obtained in the growth regulator combination of 2, 4-D (0.5mg/L) + BAP (0.1mg/L) and least (14.29%) in IAA (2.0mg/L) + BAP (0.5mg/L). Equal and higher concentration of 2, 4-D + BAP of 5.0mg/L each also provided better result (40.00%). No callus was obtained in all the single concentration of 2, 4-D used.

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“…Previously, only 70-75% survival rate of acclimatized turmeric plantlets has been reported, and poorly acclimatized plantlets are not adapted to field conditions (only 45-50% survival rate, Perić et al 2012). Alternatively, in vitro microrhizome induction in turmeric plants has been reported as one of the most important techniques to improve the survival rate in acclimatized greenhouse conditions (Hashemy et al 2009) but it is low frequency, leading to consume the long-period for microrhizome production (>18 months). In the present study, we aimed to produce the healthy plantlets of two turmeric cultivars, 'PB009' and 'ST018,' using iso-osmotic adjustment in the culture medium by several sugar types before transplanting into soil and validating the physiological adaptations, biochemical changes (including curcuminoids in minirhizome), and growth characteristics.…”

Section: Introductionmentioning

confidence: 99%

In vitro acclimatization of <i>Curcuma longa</i> under controlled <i>iso</i>-osmotic conditions

Chintakovid

Tisarum

Samphumphuang

et al. 2021

Plant Biotechnology

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In vitro acclimatization has been validated as the successful key to harden the plantlets before transplanting to ex vitro conditions. In the present study, we investigated the potential of different sugar types (glucose, fructose, galactose, sucrose) in regulating morphological, physiological and biochemical strategies, survival percentage and growth performance, and rhizome traits of turmeric under iso-osmotic potential. Leaf greenness (SPAD value) in acclimatized plantlets (4% glucose; −1.355 MPa osmotic potential) of 'ST018' was retained and greater than in 'PB009' by 1.69-fold, leading to maintain high F v /F m (maximum quantum yield of PSII), Φ PSII (photon yield of PSII) and P n (net photosynthetic rate) levels, and retained shoot height, leaf length, leaf width, shoot fresh weight and shoot dry weight after one month upon transplanting to ex vitro conditions. In addition, P n , C i (intracellular CO 2), g s (stomatal conductance) and E (transpiration rate) in acclimatized plantlets (6% sucrose; −1.355 MPa osmotic potential) of 'PB009' were stabilized as physiological adapted strategies, regulating the shoot and root growth and fresh and dry weights of mini-rhizome. Interestingly, the accumulation of total curcuminoids in mini-rhizome derived from 6% sucrose acclimatized plantlets of 'ST018' was greater than in 'PB009' by 3.76-fold. The study concludes that in vitro acclimation of turmeric 'PB009' and 'ST018' using 6% sucrose and 4% glucose, respectively, promoted percent survival, physiological adaptations, and overall growth performances under greenhouse conditions.

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Effects of light and cytokinin on in vitro micropropagation and microrhizome production in turmeric (Curcuma longa L.)

Cited by 9 publications

References 16 publications

Production of Curcuminoids in Different in vitro Organs of Curcuma longa

Production of Curcuminoids in Different in vitro Organs of Curcuma longa

Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

In vitro acclimatization of <i>Curcuma longa</i> under controlled <i>iso</i>-osmotic conditions

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