Effects of light cycle on circadian feeding activity and digestive physiology in Haliotis discus hannai

Gao, Xiaolong; Pang, Gewen; Luo, Xuan; Wang, You; Ke, Caihuan

doi:10.1016/j.aquaculture.2021.736642

Cited by 16 publications

(12 citation statements)

References 55 publications

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“…Existing research has shown that the increase of enzyme genes expression at night was the regulation of translation process and may be the anticipation of next food intake (José Antonio et al, 2016). A recent research on the feeding and digestion physiology of abalone H. discus hannai under the light/dark cycle found that digestive enzymes and feeding related genes were significantly higher at night than during the day with rhythmic 24 h oscillations (Gao et al, 2021). In this research, the mRNA levels of four key digestive enzyme genes (AMY, LPS, CEL, and PEP) by qRT-PCR during the light/dark cycles were basically consistent with the production of their enzymes.…”

Section: Discussionsupporting

confidence: 71%

“…For example, Manila clam Ruditapes philippinarum exhibited a 24 h circadian rhythm of feeding rates, having a higher feeding rate at night than that in the day (Jiang et al, 2009). A study on circadian feeding activity and digestive physiology in the abalone Haliotis discus hannai found that the percentages of ingesting food were significantly higher at night than in the daytime, demonstrating their nocturnal characteristics (Gao et al, 2021). An obvious circadian rhythm in water filtration rate was also found in the scallop Chlamys farreri, which had a significantly higher water filtration rate at night than that in daytime (Du et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

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The Discovery of Circadian Rhythm of Feeding Time on Digestive Enzymes Activity and Their Gene Expression in Sinonovacula constricta Within a Light/Dark Cycle

et al. 2021

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The circadian rhythm has a great impact on the growth, metabolism and development of animals, but little is known about the circadian rhythm of marine bivalves. Understanding of the feeding rhythm is of great significance to increase the yield of razor clam Sinonovacula constricta, an economically important bivalve mollusk. The aim of this experiment was to study the effects of circadian rhythm of feeding time on digestive enzymes activities and their gene expression in S. constricta within a light (ZT8-ZT20)/dark (ZT20-ZT8) cycle. The present results showed that circadian rhythm of feeding rate (FR) was highly associated with digestive enzyme activities and relative expression of their genes. The highest values of FR were basically observed in the night from ZT0-ZT2 and ZT6-ZT8, which were significantly higher than those values in the daytime from ZT12-ZT14 and ZT18-ZT20 (P < 0.05). The digestive enzymes activities displayed the highest values at ZT2 and ZT8, and the lowest at ZT14 and ZT20. Among them, cellulase and pepsin were found to have significantly different activities (P < 0.05), rather than amylase and lipase. Notably, the relative expression of digestive enzyme genes shared the similar pattern with the activities of digestive enzymes. The highest values of relative gene expression of amylase (AMY), lipase (LIP), cellulase (CEL), and pepsin (PEP) were found at ZT2 and ZT8 in the night, while the lowest values were found at ZT14 during the day. It is therefore suggested that the biological clock may regulate the process from feeding to digestion. Furthermore, it might be better to feed at night to reduce cultivating cost and increase economic benefits in the farming industry of S. constricta.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

The Discovery of Circadian Rhythm of Feeding Time on Digestive Enzymes Activity and Their Gene Expression in Sinonovacula constricta Within a Light/Dark Cycle

et al. 2021

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“…These findings suggest that NPF production induces feeding behavior with a consequent increase of food intake, as observed across bilaterian species. In fact, feeding rhythms, ingestion rate, and digestive enzyme activities were positively correlated with the gene expression levels of Hdh-NPF in Pacific abalone 62 and injection of NPF peptide was reported to significantly increase the filter feeding rate in the molluscan bivalve R. philippinarum 31 . In contrast, Ls-NPF showed no short-term effect on food intake in L. stagnalis 33 , 34 and its role seems to be quite different from those of abalone NPF and mammalian NPY 9 , 10 .…”

Section: Discussionmentioning

confidence: 99%

NPF activates a specific NPF receptor and regulates food intake in Pacific abalone Haliotis discus hannai

Kim

Park³

et al. 2021

Sci Rep

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Neuropeptides function through G protein-coupled receptors (GPCRs) with high specificity, implying a significant degree of neuropeptide-GPCR coevolution. However, potential neuropeptide signaling systems in non-chordates are relatively elusive. We determined the specificity of the neuropeptide F (Hdh-NPF) signaling system with a cognate receptor (Hdh-NPFR) in the Pacific abalone, Haliotis discus hannai. Phylogenetic and exon–intron arrangement analyses of bilaterian NPF and the chordate ortholog NPY with their receptor sequences revealed a likely common ancestor, and Hdh-NPFR was similar to the NPYR2 subtype among the NPYR1, NPYR2, and NPYR5 subtypes. Among four Hdh-NPFR-related receptors, Hdh-NPFR specifically responded to Hdh-NPF peptide, supported by the dose–response luciferase reporter curve, intracellular Ca2+ mobilization, and phosphorylation of ERK1/2 and its inhibition with a protein kinase C inhibitor. Peptide fragmentations and shuffling of Hdh-NPF with human NPY could not activate the cellular response of Hdh-NPFR. Three-dimensional in silico modeling suggested that interaction of Hdh-NPF C-terminal amino acids with the extracellular loops of Hdh-NPFR is critical for Hdh-NPFR activation. In vivo injection of Hdh-NPF peptide increased food consumption, and knockdown of Hdh-NPF expression decreased food consumption in Pacific abalone. These findings provide evidence for co-evolution of the NPF/Y ligand-receptor system, enabling further research on mollusk orexigenic neuropeptides.

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“…One unit of cellulase activity is defined as per g tissue to catalyze 1 μg glucose per min. One unit of α-amylase activity is defined as 1 mg reducing sugar catalyzed per min per unit of tissue ( Gao et al, 2021 ). Protein content in the homogenate was determined using Coomassie blue staining, with bovine serum albumin as the protein marker ( Bradford, 1976 ).…”

Section: Methodsmentioning

confidence: 99%

“…Digestive enzyme activity also reflects the digestive and absorptive capacity of the intestine, which in turn determines feeding and growth of animals ( Baiao et al, 2017 ; Ydsr et al, 2019 ). Digestive enzymes are known to influence diet, growth, and many other traits of abalone ( Gao et al, 2016 , 2021 ). In addition, intestinal microbiota can influence the production of some digestive enzymes to some extent ( Ekhart et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Exploring the Intestinal Microbiota and Metabolome Profiles Associated With Feed Efficiency in Pacific Abalone (Haliotis discus hannai)

Shen

et al. 2022

Front. Microbiol.

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Feed efficiency (FE) is critical to the economic and environmental benefits of aquaculture. Both the intestines and intestinal microbiota play a key role in energy acquisition and influence FE. In the current research, intestinal microbiota, metabolome, and key digestive enzyme activities were compared between abalones with high [Residual feed intake (RFI) = −0.029] and low FE (RFI = 0.022). The FE of group A were significantly higher than these of group B. There were significant differences in intestinal microbiota structures between high- and low-FE groups, while higher microbiota diversity was observed in the high-FE group. Differences in FE were also strongly correlated to variations in intestinal digestive enzyme activity that may be caused by Pseudoalteromonas and Cobetia. In addition, Saprospira, Rhodanobacteraceae, Llumatobacteraceae, and Gaiellales may potentially be utilized as biomarkers to distinguish high- from low-FE abalones. Significantly different microorganisms (uncultured beta proteobacterium, BD1_7_clade, and Lautropia) were found to be highly correlated to significantly different metabolites [DL-methionine sulfoxide Arg-Gln, L-pyroglutamic acid, dopamine, tyramine, phosphatidyl cholines (PC) (16:0/16:0), and indoleacetic acid] in the high- and low-FE groups, and intestinal trypsin activity also significantly differed between the two groups. We propose that interactions occur among intestinal microbiota, intestinal metabolites, and enzyme activity, which improve abalone FE by enhancing amino acid metabolism, immune response, and signal transduction pathways. The present study not only elucidates mechanisms of variations in abalone FE, but it also provides important basic knowledge for improving abalone FE by modulating intestinal microbiota.

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Effects of light cycle on circadian feeding activity and digestive physiology in Haliotis discus hannai

Cited by 16 publications

References 55 publications

The Discovery of Circadian Rhythm of Feeding Time on Digestive Enzymes Activity and Their Gene Expression in Sinonovacula constricta Within a Light/Dark Cycle

The Discovery of Circadian Rhythm of Feeding Time on Digestive Enzymes Activity and Their Gene Expression in Sinonovacula constricta Within a Light/Dark Cycle

NPF activates a specific NPF receptor and regulates food intake in Pacific abalone Haliotis discus hannai

Exploring the Intestinal Microbiota and Metabolome Profiles Associated With Feed Efficiency in Pacific Abalone (Haliotis discus hannai)

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