Effects of light intensity and wavelength on the production of lactobionic acid from whey by Pseudomonas taetrolens in batch cultures

Abstract

BACKGROUND Lactobionic acid production from dairy waste using Psedomonas taetrolens has gained rapid momentum as it is an economically feasible and environmentally friendly approach. Although photoreceptors have been identified in P. taetrolens, the effect of light on the production of lactobionic acid has not been investigated. This research successfully demonstrates the influence of light on lactobionic acid production by P. taetrolens from cheese whey. RESULTS Experimental results indicated that while red light promoted both cell growth and lactobionic acid production, blue light could only promote cell growth. Xmax and Pmax increased from 12.78 to 15.83 g L−1 and 17.4 to 24.5 g L−1, respectively, under red LED light at 500 lux. On the other hand, Xmax increased from 12.78 to 15.18 g L−1, but Pmax decreased from 17.4 to 15.4 g L−1 under blue LED light at 100 lux. A novel two‐stage LED photo‐bioreactor process was successful in reducing fermentation time by 24 h compared with that of the photo‐bioreactor under red LED light at 500 lux. CONCLUSION It is proposed that light of varying wavelengths and intensities can be used as modulator to enhance lactobionic production by P. taetrolens using cheese way. © 2017 Society of Chemical Industry

Downstream Approach Routes for the Purification and Recovery of Lactobionic Acid

“…However, as most biological systems, these methods still need to be improved so that they overcome the connate barriers to the process and achieve efficiency [ 5 , 6 ]. The biotechnological approach to LBA production from whey not only reduces the production costs, but also plays a significant role in the bio-remediation of waste [ 7 ]. The research findings indicate that the microbial bioconversion of lactose into LBA with Pseudomonas taetrolens is successful when using whey as a substrate [ 5 ].…”

Analysis of light absorption and photosynthetic activity by Isochrysis galbana under different light qualities

“…Light emitting diodes (LEDs) are characterized by wavelength specificity and narrow bandwidth, small mass and solid state construction, and long life. They produce minimum heating which makes research on the effects of light quality on microalgal growth and development convenient, accurate and comprehensive (Guo et al, 2008; Shu, Tseng, & Jaiswal, 2018). It is worth mentioning that the light quality in the present study is defined as the spectral distribution of photons, as adopted by previous studies (Li, Li, Liu, & Qin, 2020; Meng, Cui, Wang, & Li, 2018; Romero‐Romero & Sánchez‐Saavedra, 2017; Smith, 1982).…”