Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis, and Candida shehatae

Biological activities of lignin hydrolysate-related compounds

“…Furfural also affects the activity of certain enzymes, particularly glycolytic enzymes and dehydrogenases, suggesting that these enzymes are more sensitive and that they are probably responsible for the inhibition of alcohol production (91). Moreover, the inhibitive effects of furfural on microbial growth and metabolism in ethanol fermentation have been studied with various organisms, e.g., Saccharomyces cerevisiae (92)(93)(94)(95)(96), Pachysolen tannophilus (97) and E. coli (98), as well as with cellulase production in Trichoderma reesei (99). The addition of furfural also inhibited ABE production by Clostridium acetobutylicum (100) and xylitol production by C. guilliermondiA (101).…”

“…A study on the toxicity and detoxification of both furfural and HMF was conducted with seventeen different enteric bacterial strains, including the genera Klebsiella, Enterobacter, Escherichia, Citrobacter, Edwardsiella and Proteus (104), while the effects of HMF on the growth rate and final cell densities of the fermentative strain, Zymomonas mobilis 8b, was studied (105). HMF was also found to inhibit the growth, decrease the metabolic activity and reduce the ethanol production of various fermentative microorganisms, including Saccharomyces cerevisiae (95,96) Zymomonas mobilis, Pichia stipitis, Candida shehatae (95) and E. coli (98). In all the experiments, furfural and HMF had inhibitory effects on the strains.…”

Design and Optimization of Ethanol Production from Bagasse Pith Hydrolysate by a Thermotolerant Yeast Kluyveromyces sp. IIPE453 Using Response Surface Methodology

“…Furthermore, S. stipitis also has the natural ability to metabolize some of the sugar degradation compounds present in the hydrolysate after pretreatment (Almeida et al 2008;Wan et al 2012). The sensitivity of Scheffersomyces stipitis to inhibitors found in lignocellulose hydrolysate has been reported elsewhere (Bellido et al 2011;Delgenes et al 1996).…”

“…It was previously reported that prolonged incubation helps to acclimatize Scheffersomyces stipitis to these toxic compounds (Delgenes et al 1996). In the present study, we investigated conversion of both hexose and pentose sugars in the enzymatic hydrolysates of wet exploded sugarcane bagasse without detoxification of the inhibitors to study cell growth and ethanol yields by S. stipitis CBS6054.…”

Microbial conversion of sugars from plant biomass to lactic acid or ethanol

“…A number of excellent reviews have described various lignocellulose pre-treatment protocols used to enhance enzyme accessibility for plant cell-wall deconstruction (Kenealy et al, 2007;McMillan, 1994a;Mosier et al, 2005). It is worth mentioning that other compounds are generated as by-products of pretreatment regimes, and some of these compounds may be inhibitory to both the fermenting organism and the enzymes used for breaking polymers down to dimers or monomers (Almeida et al, 2007;Delgenes et al, 1996;Mussatto and Roberto, 2004).…”