Effects of Lipopolysaccharide on Production of Interleukin-1 and Interleukin-6 by Bovine Mammary Epithelial Cells in vitro

Okada, Hiroyuki; Ohtsuka, Hiromichi; Konnai, Satoru; Kirisawa, Rikio; Yokomizo, Y.; Yoshino, Tomo-p; Rosol, Thomas J.

doi:10.1292/jvms.61.33

Cited by 28 publications

(22 citation statements)

References 14 publications

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“…In vitro, LPS stimulation increased the production of both IL-1 and IL-6 from cryopreserved MEC in a dose-dependent manner [118]. IL-1 and IL-6 are both important mediators of inflammation including stimulation of the acute phase response and in vivo, IL-1β was detected in milk from cows suffering from mastitis [6,7,153].…”

Section: Contribution Of Mammary Epithelial Cellsmentioning

confidence: 90%

Innate immunity of the bovine mammary gland

2006

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-Understanding the immune defenses of the mammary gland is instrumental in devising and developing measures to control mastitis, the major illness of dairy ruminants. Innate immunity is an extremely broad field for investigation, and despite decades of research, our present knowledge of the innate defenses of the udder is incomplete. Yet, information is being gained on the recognition of pathogens by the mammary gland, and on several locally inducible defenses. The contribution of mammary epithelial cells to local defenses and to the mobilization of leucocytes is under growing scrutiny. Interactions of mastitis-causing bacteria such as Escherichia coli or Staphylococcus aureus and the mammary gland represents a suitable model for studies on innate immunity at an epithelium frontier. Powerful new research tools are radically modifying the prospects for the understanding of the interplay between the mammary gland innate defenses and mastitis-causing bacteria: genetic dissection of the immune response, microarray gene technology, transcriptomic methodologies and gene silencing by RNA interference will make possible the discovery of several of the key defense mechanisms which govern the susceptibility/resistance to mastitis at the molecular and genetic levels. It should then be possible to enhance the resistance of dairy ruminants to mastitis through immunomodulation and genetic improvement. mastitis / cattle / innate immunity / inflammation / milk

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Section: Contribution Of Mammary Epithelial Cellsmentioning

confidence: 90%

Innate immunity of the bovine mammary gland

2006

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“…This makes them a suitable model to address the effect of soluble mediators and ET on mammary cell function (Talhouk et al 2000b(Talhouk et al 2001. In contrast to other studies on either primary bovine mammary cells or MAC-T bovine epithelial cell line (Boudjellab et al 1998, Okada et al 1999) that where limited to monitoring the effect of ET on cytokine production, in this study, we monitored, in addition to cytokine and NGF production, the effect of ET on NF-kB activation and on the differentiation phenotype as indicated by b-casein and gelatinase expression. The data presented in this study showed that daily exposure of confluent CID-9 cells on EHS-drip to ET concentrations up to 10 mg/ml did not alter the apparent morphology (data not shown) and the viability of CID-9 cells.…”

Section: Discussionmentioning

confidence: 99%

The effect of endotoxin on functional parameters of mammary CID-9 cells

Safieh‐Garabedian¹,

Mouneimne²,

El-Jouni³

et al. 2004

Reproduction

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The effect of endotoxin on mammary CID-9 cells, which differentiate in culture and express b-casein, was investigated. Cells in culture supplemented with lactogenic hormones and dripped with EMS-Matrix (EMS-drip), were treated daily with endotoxin (0.5 -500 mg/ml). Endotoxin at concentrations of less or equal to 10 mg/ml did not affect cell growth and viability up to 5 days post endotoxin treatment. Endotoxin (0.01 -10 mg/ml) was added to the culture medium, upon confluence, and functional parameters were examined within 48 h post endotoxin treatment. Nuclear factor-kB (NF-kB) (p52) increased in nuclear extracts from endotoxin-stimulated cells within 1 h of treatment, while b-casein mRNA and protein expression decreased in a concentration-dependent manner at 24 and 48 h post treatment. Zymography showed that the 72 and 92 kDa gelatinase activity increased in cells at 24 and 48 h post endotoxin treatment at 10 and 50 mg/ml. At the latter concentration, the active form of 72 kDa gelatinase was induced at 48 h. Interleukin-6 and tumor necrosis factor-a levels increased at 1-3 h post endotoxin treatment and peaked at 6 h in cells on plastic and EHS-drip. Nerve growth factor (NGF) levels increased in control and endotoxin-treated cells in a time-dependent manner, and endotoxin increased NGF levels in culture at 6 and 9 h post endotoxin treatment. This study shows that endotoxin activated NF-kB, suppressed b-casein expression and upregulated gelatinases, cytokines and NGF. This model could be used to investigate the role of mammary cells in initiating and propagating inflammation and to test candidate molecules for potential anti-inflammatory properties.

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“…Inflammatory cytokines play important roles in the outcome of infectious diseases and inflammation [9]. The inflammatory cytokines IL-1, IL-6 and TNF-α are the principal mediators of natural immunity against gram-negative bacteria and are key mediators of inflammatory response and septic shock [14]. Susceptibility or tolerance to various infectious diseases is associated with the expression of particular cytokine profiles.…”

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confidence: 99%

Cytokine Responses in Camels (Camelus bactrianus) Vaccinated with Brucella abortus strain 19 Vaccine

Odbileg

Purevtseren²,

Dorj

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. In the present study, we determined the levels of cytokines produced by camel (Camelus bactrianus) peripheral blood mononuclear cells (PBMCs) in response to live attenuated Brucella abortus (B. abortus) S19 vaccine. Seven camels were vaccinated with commercial B. abortus S19 vaccine, and their cytokine responses were determined using a real-time PCR assay. Cytokine responses to B. abortus S19 were examined at 6 hr, 48 hr and 1, 2 and 3 weeks post-vaccination. Serological tests were performed to further confirm these immune responses. The results revealed that IFN-γ and IL-6 were upregulated during the first week post-vaccination. Low level expressions of IL-1α, IL-1β, TNFα and IL-10 and no expression of IL-2 and IL-4 were observed compared with the control camels. The findings showed that B. abortus stimulates cell-mediated immunity by directly activating camel Th1 cells to secrete IFN-γ. This quantification of cytokine expression in camels is essential for understanding of Camelidae disease development and protective immune responses. This is the first report of in vivo camel cytokine quantification after vaccination. KEY WORDS: Brucella abortus S19, camel, cytokine.

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Effects of Lipopolysaccharide on Production of Interleukin-1 and Interleukin-6 by Bovine Mammary Epithelial Cells in vitro

Cited by 28 publications

References 14 publications

Innate immunity of the bovine mammary gland

Innate immunity of the bovine mammary gland

The effect of endotoxin on functional parameters of mammary CID-9 cells

Cytokine Responses in Camels (Camelus bactrianus) Vaccinated with Brucella abortus strain 19 Vaccine

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