Effects of lipopolysaccharide on the stearoyl-coenzyme A desaturase mRNA level in bovine primary hepatic cells

Abstract

ABSTRACT. This study aimed to compare the effects of lipopolysaccharide (LPS) on stearoyl-coenzyme A desaturase (SCD) gene expression in mouse primary hepatic cells. To obtain sufficient total RNA, primary hepatic cells were plated on 6-cm diameter-type collagen 1-coated dishes (1 x 10 6 cells per dish). The test was divided into 6 groups with 6 replications per group. The 6 groups were treated with the following volumes of LPS (0.1 mg/mL): 0, 1, 1.5, 2, 4, and 8 µL. The cells were cultured for 24 h, and the total RNA was extracted from samples. Reverse transcription polymerase chain reaction was used to analyze SCD mRNA levels. With increasing LPS amounts, the SCD mRNA expression first decreased and then increased slightly; the expression was the lowest in the 2-µL LPS condition. The SCD mRNA levels from the 4-and 8-µL LPS conditions were slightly higher than that from the 2-µL LPS condition, but the difference was not significant (P > 0.05). The SCD mRNA level from the 2-µL LPS condition was obviously 2549 Stearoyl-coenzyme A desaturase mRNA ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 2548-2554 (2014) lower than that from the 0-, 1-, and 1.5-µL LPS condition, and the differences were significant (P < 0.05), and the SCD mRNA levels from the 0-, 1-, and 1.5-µL LPS conditions were not significantly different (P > 0.05). The SCD mRNA levels from the 4-and 8-µL LPS conditions were obviously lower than those from the 0-and 1-µL LPS conditions, and the differences were significant (P < 0.05).

Lipopolysaccharide derived from the rumen down-regulates stearoyl-CoA desaturase 1 expression and alters fatty acid composition in the liver of dairy cows fed a high-concentrate diet

“…Previous studies demonstrated that LPSs stimulate the gene expression of fatty acid synthetase (FAS) and acetyl-CoA carboxylase (ACC) in the liver of mice [ 5 ] but depressed stearoyl-CoA desaturase (SCD) expression in bovine hepatocytes [ 6 ]. SCD is a rate-limiting enzyme that catalyzes the synthesis of the monounsaturated fatty acids oleate (18:1) and palmitoleate (16:1) and forms triglycerides and cholesterol esters [ 7 ].…”