Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and influx of leukocytes in the hen ovary

Abdelsalam, Mohamed; Isobe, Naoki; Yoshimura, Y.

doi:10.3382/ps.2011-01394

Cited by 24 publications

(18 citation statements)

References 38 publications

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“…Therefore, we developed an in vitro model to investigate the timing of response to LPS on patterns of gene expression in rooster SCs. In hens, it has been reported that the oviduct, as well as ovarian follicular and stromal cells, expresses TLRs, AvBDs, as well as proinflammatory cytokines and chemokines, and the expression of these molecules was upregulated by LPS stimulation (Subedi et al 2007, Abdel Mageed et al 2008, Ozoe et al 2009, Woods et al 2009, Abdelsalam et al 2011, Nii et al 2011. Similar to the female reproductive tract, the gene expression data presented in this study revealed that rooster SCs responded to LPS stimulation.…”

Section: Discussionsupporting

confidence: 66%

“…RT-PCR and quantitative real-time PCR analyses were performed as described previously (Michailidis et al 2010). Quantitative PCR amplification of TLRs, AvBDs, and cytokine genes was performed as described previously (Ebers et al 2009, Michailidis et al 2010, Abdelsalam et al 2011, using the primers listed in Tables 1, 2 and 3. The cycling parameters were as follows: incubation at 95 8C for 3 min, followed by 45 cycles of incubation at 95 8C for 10 s, 56 8C for 8 s for TLRs and cytokines, and 58 8C for 8 s for AvBDs, 72 8C for 8 s, read at 60 8C, followed by a melt curve analysis from 65 to 95 8C with reads at intervals of 0.2 8C and 5 s to confirm amplification of single cDNA products.…”

Section: Rna Isolation Rt-pcr and Quantitative Real-time Pcr Analysesmentioning

confidence: 99%

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Activation of innate immune system in response to lipopolysaccharide in chicken Sertoli cells

Michailidis¹,

Anastasiadou²,

Guibert³

et al. 2014

Reproduction

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Sertoli cells (SCs) play an important physiological role in the testis, as they support, nourish, and protect the germ cells. As protection of the developing spermatozoa is an emerging aspect of reproductive physiology, this study examined the expression pattern of innate immune-related genes, including avian b-defensins (AvBDs), Toll-like receptors (TLRs), and cytokines, and investigated the time course of an inflammatory response in rooster SCs triggered by exposure to the bacterial endotoxin lipopolysaccharide (LPS). SCs were isolated from 6-week-old chicken, cultured in vitro, and stimulated with 1 mg/ml LPS at different time courses (0, 6, 12, 24, and 48 h). Data on expression analysis revealed that all ten members of the chicken TLR family, nine members of the AvBD family, as well as eight cytokine genes were expressed in SCs. Quantitative real-time PCR analysis revealed that LPS treatment resulted in significant induction of the expression levels of six TLRs, six AvBDs, and four cytokine genes, while two cytokine genes were downregulated and two other genes were unchanged. The increasing interleukin 1b (IL1b) production was confirmed in the conditioned medium. Furthermore, the phagocytosis of SCs was increased after LPS treatment. In conclusion, these findings provide evidence that SCs express innate immunerelated genes and respond directly to bacterial ligands. These genes represent an important component of the immune system, which could be integrated into semen, and present a distinctive constituent of the protective repertoire of the testis against ascending infections.

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Section: Discussionsupporting

confidence: 66%

Section: Rna Isolation Rt-pcr and Quantitative Real-time Pcr Analysesmentioning

confidence: 99%

Activation of innate immune system in response to lipopolysaccharide in chicken Sertoli cells

Michailidis¹,

Anastasiadou²,

Guibert³

et al. 2014

Reproduction

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“…Ovarian follicles infected with gram-negative bacteria cause ovarian disorders and the production of contaminated eggs (Abdelsalam et al, 2011). Escherichia coli is one of the main pathogens causing this problem.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Melatonin on LPS-stimulated Granulosa Cells in Japanese Quail

Isobe

Maeda

2017

Jpn. Poult. Sci.

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The aim of this study was to evaluate the potential of melatonin to protect cultured granulosa cells from the harmful effects of lipopolysaccharide (LPS) in quail. Granulosa cells isolated from Japanese quails were pretreated with or without melatonin (10 or 100 μg/mL) for 12 h and then incubated for 12 h in the absence or presence of 100 ng/mL LPS. The expression of pro-inflammatory cytokines and chemokine was detected by quantitative real-time PCR. The levels of oxidative stress biomarkers (dityrosine and nitrite) were determined by ELISA and the Griess reaction. Cell viability was quantified using an MTT assay. Additionally, the level of progesterone was measured by ELISA. We found that melatonin decreased LPS-induced expression of IL-1β, . In addition, melatonin increased the dityrosine level, but suppressed the nitrite level. Finally, melatonin administration increased the viability of LPS-stimulated granulosa cells in vitro. However, progesterone basal secretion was not significantly changed. These results suggest that melatonin protects cultured granulosa cells from LPS-induced inflammatory and oxidative stress damage and provide evidence that melatonin might have therapeutic utility in ovarian follicle infection in Japanese quail.

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“…It is assumed that T cells densities in the vagina are increased by single LPS injection and they returned to the original level within 24 h. However, frequent increase of T cells by repeated LPS injection may be associated with less decrease in T cell density, which eventually formed a pool of T cells in the mucosa. In hen ovary, CD4 + T cells, but not CD8 + T cells, were increased by 6 h after single LPS injection, followed by decreasing tendency by 12 h (Abdelsalam et al, 2011). Consequently, it is suggested that the repeated LPS injection in the vagina may lead the accumulation of T cell subsets in the lamina propria, probably under regulation of chemokines.…”

Section: Of B Cells and Macrophages By Cd4mentioning

confidence: 87%

Effects of Repeated Lipopolysaccharide Stimulation on the Development of Antigen-presenting Cells and T Cells Pool in Hen Vagina

2013

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The aim of this study was to determine how the mucosal immunity mediated by T cells is developed in the vagina. Antigen-presenting cells and T cells play important roles in the early process of host defense. Mature T cells and immature dendritic cells expressing CC chemokine receptor (CCR) 6. In contrast, immature T cells and mature dendritic cells express CCR7. It was examined whether repeated antigen stimulation is effective for development of the pool of antigen-presenting cells and T cells in the vagina. White Leghorn hens were intravaginally injected 1 (single injection group) or 5 times (repeated injection group) in 10 days with saline (control) or lipopolysaccharide (LPS). The vagina was collected 1 day after the final injection. , TCRγδ+ T cells were significantly increased in LPS-repeated injection group, however that of MHC class II + cells was not changed significantly. The expression of CCR6 was significantly upregulated in LPS-repeated injection group, however, the expression of CCR7 and MHC class II was not affected. The densities of the each T cell subset and MHC class II + cells, as well as expressions of chemokine receptor, MHC class II were not affected by single LPS injection or saline injection. These results suggest that the repeated antigen stimulation may not affect antigen-presenting ability by antigen-presenting cells, whereas it may lead influx of T cell subsets in the vagina. These accumulated T cells pool may contribute to form prequiescence of host immunity in the vaginal mucosa.

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Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and influx of leukocytes in the hen ovary

Cited by 24 publications

References 38 publications

Activation of innate immune system in response to lipopolysaccharide in chicken Sertoli cells

Activation of innate immune system in response to lipopolysaccharide in chicken Sertoli cells

Protective Effect of Melatonin on LPS-stimulated Granulosa Cells in Japanese Quail

Effects of Repeated Lipopolysaccharide Stimulation on the Development of Antigen-presenting Cells and T Cells Pool in Hen Vagina

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