Effects of Liriopis Tuber on 4-HNE-induced Apoptosis in PC-12 Cells

Abstract

Objects : Apoptosis leads to the death of a cell. The mitochondrial pathway of apoptosis, a process regulated by the Bcl-2 family of proteins, plays a key role in various biological processes. The tuber of Liriope platyphylla Wang et TANG (Liliaceae), also known as Liriopis tuber, is famous in Oriental medicine owing to its tonic, antitussive, expectorant and anti-asthmatic properties. The purpose of this study was to observe the effect of the Liriopis tuber on mitochondrial -mediated apoptosis in PC-12 cells. Method : A cytotoxic test on Liriopis Tuber water extract was conducted and another MTT assay was conducted to observe the cytoprotective effect against 4-HNE 25 µM that causes oxidative stress in PC-12 cells for 24 hours. In addition, in order to observe the expression of Bcl-2 and Bax protein involved with apoptosis, western blot was conducted. Results : The LT water extract had no toxicity for PC-12 cell. In the cytoprotective effect against 4-HNE, both of the group treated with 50 µg/㎖ and 100 µg/㎖ of LT water extract showed a significant increase in comparison with the control group. In Bax protein expression, all the experimental groups treated with LT water extract showed a decrease in comparison with the control group but had no significance. In Bcl-2 protein expression, all the experimental groups treated with LT water extract showed a significant increase in comparison with the control group. Conclusion : These results suggest that LT is effective in reducing apoptosis.

Single Intramuscular-dose Toxicity of Samgihwalryeok-Pharmacopuncture in Sprague-Dawley Rats

“…Decreased cerebral infarction or ischemia damage [13] and improvement in hypothyroidism induced by thiourea [14] were reported in Angelica gigas pharmacopuncture studies. Liriope platyphylla was reported to have anti-inflammation [15] and anti-cancer effects [16], and Schisandra chinensis Baillon was reported to have anti-inflammation effects [17]. Samgihwalryeok pharmacopuncture made with all of these was reported to affect chronic fatigue and insomnia [4].…”

Phytochemical and pharmacological overview on <i>Liriopes radix</i>

“…ERK I/II and insulin receptor were markedly activated by LR (fractions T, A, C, and M) [1]. Water extract of LR had no toxicity on PC12 cells and showed a nonsignificant decrease in Bax, and a significant increase in Bcl-2 against 4-hydroxynonenal (4-HNE) [54]. Ethanol extract of LR attenuated the H 2 O 2 -induced increases of intracellular oxidative stress and mitochondrial dysfunction.…”

Anti-Inflammatory Effect of Combination of Scutellariae Radix and Liriopis Tuber Water Extract

“…Liriopis Tube is reported to have diverse medical actions, such as antidiabetic effect, anti-inflammation, and suppressing Ig M antibody production [ 11 , 24 – 26 ]. Rhee et al reported that anti-inflammatory action, in particular, of the extract of Liriopis Tube is effective in pulmonary fibrosis by inflammation adjustment via increasing macrophages ratio and decreasing lymphocytes and neutrophils ratios [ 11 ].…”