[Purpose] The aim of this study was to investigate the impact of different marathon
running distances (10 km, 21 km, and 42.195 km) on muscle and lymphocyte DNA damage in
amateur marathon runners. [Subjects and Methods] Thirty male amateur runners were randomly
assigned to 10 km, 21 km, and 42 km groups, with 10 subjects in each group. Blood samples
were collected before and after the races and on the 3rd day of recovery to examine levels
of muscle damage (creatine kinase and lactate dehydrogenase) and lymphocyte DNA damage
(DNA in the tail, tail length, and tail moment). [Results] Serum creatine kinase, serum
lactate dehydrogenase, and tail moment were significantly higher after the races compared
with before the races in all groups. In addition, the 42 km group showed significantly
higher levels of creatine kinase, lactate dehydrogenase, and tail moment than the 10 km
and 21 km groups after the races. [Conclusion] Strenuous endurance exercise can cause
muscle and lymphocyte DNA damage, and the extent of such damage can increase as running
distance increases.