Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI

Krueger, Eddy; Magri, L.M.S.; Botelho, A.S.; Bach, Fernando Swiech; Rebellato, Carmen Lucia Kuniyoshi; Fracaro, Letícia; Fragoso, Felipe Yukio Ishikawa; Villanova, José Ademar; Brofman, Paulo Roberto Slud; Popović-Maneski, Lana

doi:10.1016/j.neulet.2018.12.004

Cited by 20 publications

(20 citation statements)

References 45 publications

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“…Functional electrical stimulation has not yet been reported in dogs, but a wearable device is being developed that might be useful in dogs with incomplete recovery from acute IVDH (80). Short-term, low-intensity electrical stimulation of the spinal cord with or without stem cell transplantation has been performed in chronically paraplegic dogs (81). While electromyographic changes in pelvic limb muscles implied improvement in motor conduction, further investigation would be necessary to optimize therapy and determine if there is a clinical benefit (81).…”

Section: Functional Electrical Stimulationmentioning

confidence: 99%

“…Short-term, low-intensity electrical stimulation of the spinal cord with or without stem cell transplantation has been performed in chronically paraplegic dogs (81). While electromyographic changes in pelvic limb muscles implied improvement in motor conduction, further investigation would be necessary to optimize therapy and determine if there is a clinical benefit (81). Additionally, there is experimental evidence and data in dogs with naturally-occurring injury for electrical stimulation of peripheral nerves or nerve roots to aid in restoration of urination and defecation (82)(83)(84).…”

Section: Functional Electrical Stimulationmentioning

confidence: 99%

“…Since 2014, institutions in Brazil have reported seven trials testing safety or efficacy of various cell transplants (within the spinal cord or in the sub-arachnoid or epidural space) in small series of companion dogs, sometimes with concomitant spinal cord decompression, or in association with other alternative therapies such as electroacupuncture (77) or lowintensity electrical stimulation (81). The cells tested have been either autologous bone marrow mesenchymal stem cells (147), allogenic fetal bone marrow stem cells (148), allogenic canine adipose tissue-derived mesenchymal stem cells (81,149,150) or immature dental pulp stem cells (77,151).…”

Section: Cell Transplantation Within the Spinal Cord In Dogs With Natmentioning

confidence: 99%

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Emerging and Adjunctive Therapies for Spinal Cord Injury Following Acute Canine Intervertebral Disc Herniation

2020

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Some dogs do not make a full recovery following medical or surgical management of acute canine intervertebral disc herniation (IVDH), highlighting the limits of currently available treatment options. The multitude of difficulties in treating severe spinal cord injury are well-recognized, and they have spurred intense laboratory research, resulting in a broad range of strategies that might have value in treating spinal cord-injured dogs. These include interventions that aim to directly repair the spinal cord lesion, promote axonal sparing or regeneration, mitigate secondary injury through neuroprotective mechanisms, or facilitate functional compensation. Despite initial promise in experimental models, many of these techniques have failed or shown mild efficacy in clinical trials in humans and dogs, although high quality evidence is lacking for many of these interventions. However, the continued introduction of new options to the veterinary clinic remains important for expanding our understanding of the mechanisms of injury and repair and for development of novel and combined strategies for severely affected dogs. This review outlines adjunctive or emerging therapies that have been proposed as treatment options for dogs with acute IVDH, including discussion of local or lesion-based approaches as well as systemically applied treatments in both acute and subacute-to-chronic settings. These interventions include low-level laser therapy, electromagnetic fields or oscillating electrical fields, adjunctive surgical techniques (myelotomy or durotomy), systemically or locally-applied hypothermia, neuroprotective chemicals, physical rehabilitation, hyperbaric oxygen therapy, electroacupuncture, electrical stimulation of the spinal cord or specific peripheral nerves, nerve grafting strategies, 4-aminopyridine, chondroitinase ABC, and cell transplantation.

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Section: Functional Electrical Stimulationmentioning

confidence: 99%

Section: Functional Electrical Stimulationmentioning

confidence: 99%

Section: Cell Transplantation Within the Spinal Cord In Dogs With Natmentioning

confidence: 99%

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Emerging and Adjunctive Therapies for Spinal Cord Injury Following Acute Canine Intervertebral Disc Herniation

2020

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“…Chen et al [31] performed electroacupuncture on rats with spinal cord injury and found that this treatment is effective to prevent oligodendrocyte apoptosis and to improve functional recovery after spinal cord injury. Krueger et al [32] performed the association of electrostimulation with mesenchymal stromal cells derived from adipose tissue in dogs with SCI and observed improvement, but without statistical difference between the associated treatments (electrostimulation and MSCs) and isolated.…”

Section: Treatmentsmentioning

confidence: 99%

Mesenchymal stromal cells as a choice for spinal cord injury treatment

Fracaro¹,

Zoehler²,

Rebelatto³

2020

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Spinal cord injury (SCI) is a serious clinical problem that affects approximately 17,500 new patients per year in the United States. The main causes of SCI are vehicle collisions, falls, violence (mainly gunshot wounds), and sports/recreational activities. The final severity of the damage results from primary and secondary mechanisms that begin at the time of injury and last for months after trauma. To reduce the extent of damage, several treatments have been proposed. This review summarizes results from several studies that have pointed to cell therapy as the main form of neuroregenerative treatment. Mesenchymal stromal cells (MSCs) are important candidates for tissue regeneration due to the release of bioactive factors, as well as antiapoptotic effects, scar inhibitors, and angiogenic effects. Studies have shown that MSCs act in various ways on injured tissue, such as immunomodulation of the inflamed environment, release of bioactive factors, restoration of axon myelin, prevention of neuronal apoptosis, and neuroregeneration. Current research using MSCs aims to prevent secondary injury, promote regeneration, and replace destroyed spinal cord tissue. This review presents information about the damage from primary and secondary events after SCI, treatments usually used, and preclinical and clinical results aiming at the cell therapy using MSCs as a tissue regeneration strategy.

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“…Intralimb hip–knee coordination may be promoted by the combination of FES and locomotor training [ 37 , 38 , 39 ]. In chronic patients, electrical stimulation protocols with FES and Transcutaneous Electrical Spinal Cord Stimulation (TESCS) could be applied as a possible therapeutic neurorehabilitation tool for IVDD [ 40 , 41 , 42 , 43 ]. TESCS can lead to synergistic and interactive multi-segmental effects, converging with the ascending sensorial pathways and the descending motor pathways, that might be important in activating the spinal network [ 44 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Neurorehabilitation in Dogs with an Incomplete Recovery 3 Months following Intervertebral Disc Surgery: A Case Series

Martins

Gouveia²,

Cardoso³

et al. 2021

Animals

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This case series study aimed to evaluate the safety, feasibility, and positive outcome of the neurorehabilitation multimodal protocol (NRMP) in 16 chronic post-surgical IVDD Hansen type I dogs, with OFS 0/DPP− (n = 9) and OFS 1/DPP+ (n = 7). All were enrolled in the NRMP for a maximum of 90 days and were clinically discharged after achieving ambulation. The NRMP was based on locomotor training, functional electrical stimulation, transcutaneous electrical spinal cord stimulation, and 4-aminopyridine (4-AP) pharmacological management. In the Deep Pain Perception (DPP)+ dogs, 100% recovered ambulation within a mean period of 47 days, reaching OFS ≥11, which suggests that a longer period of time is needed for recovery. At follow-up, all dogs presented a positive evolution with voluntary micturition. Of the DPP− dogs admitted, all achieved a flexion/extension locomotor pattern within 30 days, and after starting the 4-AP, two dogs were discharged at outcome day 45, with 78% obtaining Spinal Reflex Locomotion (SRL) and automatic micturition within a mean period of 62 days. At follow-up, all dogs maintained their neurological status. After the NRMP, ambulatory status was achieved in 88% (14/16) of dogs, without concurrent events. Thus, an NRMP may be an important therapeutic option to reduce the need for euthanasia in the clinical setting.

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Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI

Abstract: Low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI,

Cited by 20 publications

References 45 publications

Emerging and Adjunctive Therapies for Spinal Cord Injury Following Acute Canine Intervertebral Disc Herniation

Emerging and Adjunctive Therapies for Spinal Cord Injury Following Acute Canine Intervertebral Disc Herniation

Mesenchymal stromal cells as a choice for spinal cord injury treatment

Functional Neurorehabilitation in Dogs with an Incomplete Recovery 3 Months following Intervertebral Disc Surgery: A Case Series

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