Effects of low-intensity laser irradiation on the apoptosis of rabbit vascular smooth muscle cells in culture

Li, S. D.; Chen, P.; Zhang, C. P.; Jiang, Wen; Liang, Jie; Kang, Hongxiang; Gao, Rui; Fu, Xiao

doi:10.1134/s1054660x11190212

Cited by 4 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Laser-induced free radicals could unbalance cellular defenses against these chemical species causing unwanted effects in irradiated tissues by the alteration of biomolecular functions, such as DNA [8,10]. This has been reported in eukaryotic cells [11][12][13][14][15] for lasers of different powers, wavelengths, and fluences. Thus, biological systems must have protection systems against damage caused by free radicals.…”

Section: Introductionmentioning

confidence: 94%

Low-level lasers affect uncoupling protein gene expression in skin and skeletal muscle tissues

et al. 2016

View full text Add to dashboard Cite

Wavelength, frequency, power, fluence, and emission mode determine the photophysical, photochemical, and photobiological responses of biological tissues to low-level lasers. Free radicals are involved in these responses acting as second messengers in intracellular signaling processes. Irradiated cells present defenses against these chemical species to avoid unwanted effects, such as uncoupling proteins (UCPs), which are part of protective mechanisms and minimize the effects of free radical generation in mitochondria. In this work UCP2 and UCP3 mRNA gene relative expression in the skin and skeletal muscle tissues of Wistar rats exposed to low-level red and infrared lasers was evaluated. Samples of the skin and skeletal muscle tissue of Wistar rats exposed to low-level red and infrared lasers were withdrawn for total RNA extraction, cDNA synthesis, and the evaluation of gene expression by quantitative polymerase chain reaction. UCP2 and UCP3 mRNA expression was differently altered in skin and skeletal muscle tissues exposed to lasers in a wavelength-dependent effect, with the UCP3 mRNA expression dose-dependent. Alteration on UCP gene expression could be part of the biostimulation effect and is necessary to make cells exposed to red and infrared low-level lasers more resistant or capable of adapting in damaged tissues or diseases.

show abstract

Section: Introductionmentioning

confidence: 94%

Low-level lasers affect uncoupling protein gene expression in skin and skeletal muscle tissues

et al. 2016

View full text Add to dashboard Cite

show abstract

“…A second Scopus database search was undertaken to evaluate the effects of green light PBM using the terms described in Figure 3b. An initial search resulted in 236 articles being identified and these articles were subsequently screened for suitability, which then identified 32 relevant articles for further review (18,32,33,64,68,69,79,164,168,171,176,177,186,197,205,209,(212)(213)(214)(215)(216)(217)(218)(219)(220)(221)(222)(223)(224)(225)(226)(227).…”

Section: Kazemi Khoo Et Al 2013(184)mentioning

confidence: 99%

Under the spotlight: mechanisms of photobiomodulation concentrating on blue and green light

Serrage

Heiskanen

Palin

et al. 2019

Photochem Photobiol Sci

104

View full text Add to dashboard Cite

show abstract

“…Moreover, laser-induced biological effects by free radicals are still controversial. Possible adverse effects on cells and data about DNA damage after laser exposure have been reported in eukaryotic [20][21][22][23][24] and prokaryotic cells [25][26][27][28][29][30][31][32] for lasers of different powers, wavelengths, and fluences. Furthermore, a protective effect or adaptive response (as a form of presensitization or direct DNA repair in E. coli) under sustained exposure to low intensity laser irradiation on E. coli cultures is rendered less effective at subsequent high doses of harmful radiation [27,[33][34][35] or chemical agents [28].…”

Section: Introductionmentioning

confidence: 99%

Low intensity infrared laser affects expression of oxidative DNA repair genes in mitochondria and nucleus

et al. 2014

View full text Add to dashboard Cite

Practical properties and physical characteristics of low intensity lasers have made possible their application to treat soft tissue diseases. Excitation of intracellular chromophores by red and infrared radiation at low energy fluences with increase of mitochondrial metabolism is the basis of the biostimulation effect but free radicals can be produced. DNA lesions induced by free radicals are repaired by the base excision repair pathway. In this work, we evaluate the expression of POLγ and APEX2 genes related to repair of mitochondrial and nuclear DNA, respectively. Skin and muscle tissue of Wistar rats were exposed to low intensity infrared laser at different fluences. One hour and 24 hours after laser exposure, tissue samples were withdrawn for total RNA extraction, cDNA synthesis, and evaluation of POLγ and APEX2 mRNA expression by real time quantitative polymerase chain reaction. Skin and muscle tissue of Wistar rats exposed to laser radiation show different expression of POLγ and APEX2 mRNA depending of the fluence and time after exposure. Our study suggests that a low intensity infrared laser affects expression of genes involved in repair of oxidative lesions in mitochondrial and nuclear DNA.

show abstract

Effects of low-intensity laser irradiation on the apoptosis of rabbit vascular smooth muscle cells in culture

Cited by 4 publications

References 38 publications

Low-level lasers affect uncoupling protein gene expression in skin and skeletal muscle tissues

Low-level lasers affect uncoupling protein gene expression in skin and skeletal muscle tissues

Under the spotlight: mechanisms of photobiomodulation concentrating on blue and green light

Low intensity infrared laser affects expression of oxidative DNA repair genes in mitochondria and nucleus

Contact Info

Product

Resources

About