Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid

Pansani, Taísa Nogueira; Basso, Fernanda Gonçalves; Turirioni, A.P. Silveira; Kurachi, Cristina; Hebling, Josimeri; Costa, Carlos Alberto de Souza

doi:10.1016/j.ijom.2014.02.011

Cited by 28 publications

(23 citation statements)

References 29 publications

(56 reference statements)

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“…Conversely, LLLT enhanced cell migration, since the mechanically created wound area was decreased by about 30% after laser application at 1.5 and 3 J/ cm 2 . Similar data have been reported by other authors when epithelial cells [13][14][15][16][17][18] and gingival fibroblasts [11] were subjected to low-power laser irradiation. This positive effect may be caused, at least in part, by increased synthesis of some growth factors, such as vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (bFGF), and even EGF by cells subjected to laser irradiation [11].…”

Section: Discussionsupporting

confidence: 90%

“…In vitro LLLT was performed by means of a specific diode device (LaserTABLE, 780 nm, 25 mW; spot area of 2 cm 2 ; distance of 2.5 cm) at 0.5, 1.5, and 3 J/cm 2 [12,[14][15][16]. These parameters were selected based on the results from previous studies that demonstrated cell biostimulation and acceleration of cell functions related to tissue repair, such as proliferation, migration, and growth factor expression [10,11,14]. Cells were subjected to three irradiations every 24 h. All parameters of LLLT used in this investigation are shown in Table 2.…”

Section: Llltmentioning

confidence: 99%

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Epithelial cell-enhanced metabolism by low-level laser therapy and epidermal growth factor

et al. 2017

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Reepithelialization and wound closure are the desired outcome for several ulcerative conditions. Such resolution reduces the possibility of wound contamination and maintenance of the injury and improves the reestablishment of tissue morphology and functions. Investigators are seeking adjuvant therapies that can accelerate wound healing and are developing new strategies for clinical applications. This study compared the effects of epidermal growth factor (EGF) application and low-level laser therapy (LLLT) on cultured epithelial cells. Cells were seeded in 24-well plates. After a 24-h incubation, the epithelial cells were either treated with EGF (100 μM in serum-free DMEM for 72 h) or subjected to LLLT (780 nm, 25 mW, 0.5, 1.5, and 3 J/cm) by three applications every 24 h. Seventy-two hours after cells were treated with EGF or LLLT, cell migration, viability, proliferation, and collagen synthesis were assessed. Cells treated with EGF showed increased cell viability, proliferation, and collagen synthesis compared with those cells that received no treatment. LLLT enhanced cell migration; however, no significant effects of laser irradiation on other cell functions were observed. Comparison of both therapies demonstrated that EGF and LLLT enhanced specific epithelial cell activities related to wound healing.

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Section: Discussionsupporting

confidence: 90%

Section: Llltmentioning

confidence: 99%

Epithelial cell-enhanced metabolism by low-level laser therapy and epidermal growth factor

et al. 2017

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“…In vitro and in vivo studies have shown that BPs have negative effects on several cell lines and consequently compromise the ability of the tissue to perform repair. [5][6][7] Thus, the use of adjuvant therapies with biostimulatory effects could promote benefits, including the cases in which the standard protocol does not achieve satisfactory results.…”

Section: Introductionmentioning

confidence: 99%

The Effectiveness of the Low-Level Laser, Antibiotic and Surgical Therapy in the Treatment of Medication-Related Osteonecrosis of the Jaws: A Case Report

et al. 2020

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Introduction: Bisphosphonates consist of a range of drugs used in the treatment of osteopathy or some osteotropic malignancies. Medication-related osteonecrosis of the jaw (MRONJ) is a serious adverse consequence of their use. Conventional treatment is not always effective, so it is necessary to resort to the use of adjuvant therapies. This study aimed to evaluate the effectiveness of the association of surgery, antibiotic therapy and low-level laser (LLL) therapy as a treatment strategy for MRONJ through the presentation of a clinical case. Case Presentation: A 49-year-old female patient presented for the diagnosis and treatment of bone lesions in the maxillae. The patient reported that she had used zoledronate for 1 year. Five years later there were 2 painless bone lesions on both sides of the maxilla, following the extraction of the first upper molars. Clinical, tomographic and histopathologic examination established the diagnosis of MRONJ. The treatment consisted of the curettage of the necrotic bone, antibiotic and thirteen LLL therapy sessions. Integral mucosal healing was observed after a two-month follow-up and no symptoms were detected. The patient was evaluated at 6 and 12 postoperative months without complications. Conclusion: The combination of surgery, antibiotic therapy, and LLL therapy has shown to be effective in the treatment of MRONJ.

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“…In addition to growth factors, low-level laser therapy (LLLT) can also promote tissue repair, by increasing cell proliferation and expression of growth factors and also by modulating the synthesis of inflammatory cytokines by several cell types, including gingival fibroblasts [19][20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals

et al. 2016

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This study evaluated the effects of low-level laser therapy (LLLT) and epidermal growth factor (EGF) on fibroblasts obtained from young and elderly individuals. Gingival fibroblasts from young (Y) and elderly (E) individuals were seeded in wells of 24-well plates with Dulbecco's modified Eagle's medium (DMEM) containing 10 % of fetal bovine serum (FBS). After 24 h, the cells were irradiated (LASERTable-InGaAsP-780 ± 3 nm, 25 mW, 3 J/cm) or exposed to EGF (100 μM). After 72 h, cells were evaluated for viability, migration, collagen and vascular endothelial growth factor (VEGF) synthesis, and gene expression of growth factors. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (α = 5 %). Y and E fibroblasts irradiated with laser or exposed to EGF showed increased viability and collagen synthesis. Enhanced cell migration was observed for Y fibroblasts after both treatments, whereas only the LLLT stimulated migration of E cells. VEGF synthesis was higher for Y and E cells exposed to EGF, while this synthesis was reduced when E fibroblasts were irradiated. Increased gene expression of VEGF was observed only for Y and E fibroblasts treated with LLLT. Regardless of a patient's age, the LLLT and EGF applications can biostimulate gingival fibroblast functions involved in tissue repair.

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Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid

Cited by 28 publications

References 29 publications

Epithelial cell-enhanced metabolism by low-level laser therapy and epidermal growth factor

Epithelial cell-enhanced metabolism by low-level laser therapy and epidermal growth factor

The Effectiveness of the Low-Level Laser, Antibiotic and Surgical Therapy in the Treatment of Medication-Related Osteonecrosis of the Jaws: A Case Report

Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals

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