Effects of Low Molecular Weight Fucoidan on the Proliferation and Apoptosis of Dysplastic Oral Keratinocyte and Oral Squamous Cell Carcinoma Cells

Li, Lulu; Xu, Yingjie; Wang, Yixue; Liu, Mengjia; Deng, Songsong; Yu, Xixi; Cong, Beibei; Wang, Wanchun

doi:10.1177/1934578x20921681

Cited by 4 publications

(5 citation statements)

References 33 publications

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“…( Figure S4B ). These results were responding to our published works [ 46 ]. Therefore, we inferred that the effective therapeutic concentration of LMWF is between 50 and 250 μg/mL and that LMWF is a potential drug for the treatment of OSCC.…”

Section: Resultssupporting

confidence: 90%

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On-Demand Release of Fucoidan from a Multilayered Nanofiber Patch for the Killing of Oral Squamous Cancer Cells and Promotion of Epithelial Regeneration

Liu

Zhang

et al. 2022

JFB

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Oral squamous cell carcinoma represents 90% of all oral cancers. Recurrence prevention remains an important prognostic factor in patients with oral squamous cell carcinoma, and the recovery of the oral epithelium post-surgery is still a challenge. Thus, there is an urgent need to develop a smart carrier material to realize the spatiotemporally controlled release of anticancer drugs, instead of multiple oral administrations, for recurrence prevention and promoting the reconstruction of injured epithelial tissues. Here, we developed a multi-layered nanofiber patch capable of the photothermal-triggered release of low-molecular-weight fucoidan (LMWF) from the sandwiched layer, together with electrospun fibers as the backing and top layers. The sandwiched layer was made of phase-change materials loaded with indocyanine green, a photosensitive dye, for the localized release of LMWF in response to near-infrared irradiation. We showed that the on-demand release of LMWF was able to kill oral cancer cells effectively. Furthermore, adding acellular dermal matrix to the top nanofiber layer improved the proliferation of human oral keratinocytes, while the hydrophobic back layer served as a barrier to prevent loss of the drug. Taken together, this study provides a feasible and smart material system for killing oral squamous cancer cells together with the recovery of oral epithelium.

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Section: Resultssupporting

confidence: 90%

“…Our previous study proved that low-molecular high-sulfated polysaccharides exhibited cytotoxicity toward SCC-9 cells but little cytotoxicity toward HOK cells [ 46 ]. In this study, we first extracted the LMWF and analyzed the composition.…”

Section: Resultsmentioning

confidence: 99%

On-Demand Release of Fucoidan from a Multilayered Nanofiber Patch for the Killing of Oral Squamous Cancer Cells and Promotion of Epithelial Regeneration

Liu

Zhang

et al. 2022

JFB

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“…Oral keratinocytes are precursor cells of OSCC, and xenobiotic metabolizing enzymes (XME) influence the metabolism of chemicals that cause cancer [ 3 ]. The primary cause is DNA mutation, which is worsened by exposure to chemical, physical, or microbial mutagens.…”

Section: Discussionmentioning

confidence: 99%

“…Smoking and tobacco chewing are the two etiologic variables that are significant for the development of OSCC [ 2 ]. The OSCC-originating cell is the oral keratinocyte [ 3 ]. The lips, gingiva, palate, and dorsum of the tongue are common sites of OSCC [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Presentation of a 32-Year-Old Female Patient With Rapidly Growing Oral Squamous Cell Carcinoma: Report of a Rare Case

Kukde¹,

Lanjekar²,

Deotale³

et al. 2023

Cureus

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In this case report, a 32-year-old female patient from Central India was reported. Her primary complaint was pain and swelling in the lower left back region of her jaw, which had been present for one month. On extraoral examination, asymmetrical facial symmetry was observed, and a 3 cm swelling was present on the left side of her face. An intraoral examination showed a reddish-pink ulceroproliferative growth on the left buccal mucosa. Under local anesthesia, an incisional biopsy was performed. A conclusive diagnosis of well-differentiated squamous cell carcinoma affecting the left buccal mucosa was made based on clinical and histopathological testing. The patient was scheduled for a follow-up appointment after being referred to the Cancer Institute for the required treatment. After three months, the patient reported to the department with extensive swelling on the left side of the face, measuring 6 cm in diameter. On intraoral examination, a more restricted mouth opening compared to the previous examination. The ulceroproliferative growth was firm and tender and exhibited continuous bleeding. As the patient delayed seeking proper care, the condition became more aggressive, and she lost her life. This case of oral squamous cell carcinoma (OSCC) in a 32-year-old female patient is rare, as men are more commonly affected by OSCC in Central India due to their higher prevalence of unhealthy practices. This case highlights the rapid progression of the disease if appropriate treatment is not initiated promptly upon diagnosis.

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“…Understanding the effects of certain substances on the body is essential for researchers and clinicians to determine the most appropriate therapy. Many in vitro and in vivo studies have been carried out to determine the response of cells, tissues, as well as the body's environment to a material [27][28][29][30].…”

Section: Discussionmentioning

confidence: 99%

Effect of Meloxicam on the Proliferation and Apoptosis of the Raji Cell Line: an In Vitro Study

Asmarani

Supriatno

Haniastuti

2022

International Journal of Dentistry

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Meloxicam, a nonsteroidal anti-inflammatory drug, inhibits the production of PGE2 by blocking Cox-2 activity. Specific inhibition of Cox-2 can be useful in cancer therapy by apoptosis stimulation. The objective of the research was to study the effect of meloxicam on the proliferation and apoptosis of Raji cell lines. Burkitt lymphoma (BL) cells (Raji ATCC CCL-86) were treated with various concentrations of meloxicam for 24 hours. The proliferation of the cells was evaluated by using an MTT assay. Cell apoptosis was assessed using flow cytometry, and SEM was performed to observe the morphological changes of the cells. Results showed that meloxicam affected Raji cell proliferation as well as cell apoptosis. The percentage of viable cells was decreased significantly after being treated with meloxicam ( p < 0.05 ). Apoptotic cell percentage was higher in the groups treated with meloxicam compared to the control group ( p < 0.05 ). SEM showed morphological changes in the Raji cells after treatment with meloxicam, showing apoptotic characteristics. These findings suggest that meloxicam has anticancer properties by inhibiting Raji cell proliferation and inducing Raji cell apoptosis in vitro. A combination of meloxicam with chemotherapy agents may improve the outcome of BL treatment.

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Effects of Low Molecular Weight Fucoidan on the Proliferation and Apoptosis of Dysplastic Oral Keratinocyte and Oral Squamous Cell Carcinoma Cells

Cited by 4 publications

References 33 publications

On-Demand Release of Fucoidan from a Multilayered Nanofiber Patch for the Killing of Oral Squamous Cancer Cells and Promotion of Epithelial Regeneration

On-Demand Release of Fucoidan from a Multilayered Nanofiber Patch for the Killing of Oral Squamous Cancer Cells and Promotion of Epithelial Regeneration

Presentation of a 32-Year-Old Female Patient With Rapidly Growing Oral Squamous Cell Carcinoma: Report of a Rare Case

Effect of Meloxicam on the Proliferation and Apoptosis of the Raji Cell Line: an In Vitro Study

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