Effects of Low Power Laser Irradiation on Intracellular Calcium and Histamine Release in RBL‐2H3 Mast Cells

Yang, Wenzhong; Chen, Jiyao; Yu, Ji-Tong; Zhou, Leyuan

doi:10.1111/j.1751-1097.2007.00116.x

Cited by 78 publications

(60 citation statements)

References 34 publications

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“…Photodamage during microscopy indeed indicates that cells can be stimulated solely by photons. Along with this idea, it was found that low energy visible light irradiation with about 0.1 mw/cm 2 power density for seconds to minutes could induce changes of intracellular Ca 2+ concentrations in macrophages [76], sperms [77][78][79], lymphocytes [80], skeletal muscle cells [81,82], human fibroblasts [83] and mast cells [84]. The cellular Ca 2+ level slowly increased during continuous laser irradiation and could maintain for minutes.…”

Section: All-optical Stimulationmentioning

confidence: 98%

Regulation of Cellular Molecular Signaling by Light (Invited Paper)

Cheng¹,

Zhu²,

He³

2015

PIER

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Abstract-Laser technology has been promoting various microscopy methods and thus making great progresses in life science. Further than contribution to "seeing is believing", lasers have also demonstrated their capacity of manipulating cells and even molecular signaling. Specifically, with advances of lasers and combination with other techniques, recent reports show that cell calcium ion, a universal intra-and inter-cellular messenger, can be modulated by lasers at different levels of biological organization from organelle to tissue. It is very encouraging that laser irradiation can activate or control plenty of corresponding cell processes and functions by regulating cell calcium signaling pathways, with promising potential in both scientific research and clinical application. In this paper, optical techniques for regulation of cell calcium signaling are specifically reviewed. Most methods need exogenous chemicals or genetic materials to convert incident photon into stimulation that cells can response with specific molecular dynamics. The only all-optical approach is achieved by nonlinear excitation with femtosecond laser, despite lack of specificity and controllability, providing possibility of a totally noninvasive method without any biochemical materials and thus further potential clinical application in human beings. The developments and techniques of those methods are introduced and explained, with analysis on their properties and current challenges. Potential applications and prospective development are also discussed. Researchers on biophotonics and related biological fields can benefit from this review. It also provides a systematic reference to doctors and researchers who are working on practical application of those methods.

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Section: All-optical Stimulationmentioning

confidence: 98%

Regulation of Cellular Molecular Signaling by Light (Invited Paper)

Cheng¹,

Zhu²,

He³

2015

PIER

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“…Such mechanical forces may stimulate cutaneous mast cells, which are the main effector cells involved in allergic reactions and innate immunity, initiating chain physiological reactions (Malaviya and Georges, 2002). Mast cells can react quickly to an external stimuli, including physical ones like shear stress (Yang et al, 2009), light (Yang et al, 2007), UVA ) rays and heat (Wu et al, 2012) to initiate a physiological chain reaction, such as increment of [Ca 2+ ] c (cytosolic calcium concentration) and the degranulation, then release histamine and substance P, which give rise to vasodilatation, vessel permeability increment and microcirculation improvement. Therefore mast cells could be the targeting cells of the mechanical stimuli to recuperate the physiological function of bodies.…”

Section: Introductionmentioning

confidence: 99%

Fluid shear stress-induced cytosolic calcium signalling and degranulation dynamics in mast cells

Wei

Shi

Chen

et al. 2012

Cell. Biol. Int. Rep.

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A mast cell line (rat basophilic leukaemia cells, RBL-2H3) was used in vitro to study cellular responses to fluid shear stress generated by a rotating rotor in a cell dish. The [Ca 2+ ] c (cytosolic calcium concentration) in mast cells was detected by confocal fluorescence microscopy after Fluo-3/AM (acetoxymethyl ester) staining. Cytosolic calcium fluorescence intensity oscillated, pulsed, or steadily increased after the application of a weak, moderate or strong fluid shear stress respectively. From a mathematical model, we reproduced a change in [Ca 2+ ] c under different levels of fluid shear stress in mast cells. The model calculation confirmed another experimental observation, specifically, that the degranulation rate increases with shear stress and reaches certain steady values. Since mast cells can react clearly and quickly and obviously to mechanical stimuli, calcium signalling and degranulation dynamics could be one of the mechanisms of acupuncture and massage therapies.

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“…Zum blauen Laser gibt es generell bislang noch sehr wenige Studien im Bereich der Medizin. Neben Forschungen auf zellulärer Ebene in Shanghai [9] beschäftigen sich auch Wissenschafter in New York [10], Finnland [1], Deutschland [4] und Japan [11,12] mit der Thematik. Der Ansatz, den blauen Laser in der Akupunktur zu verwenden, ist neu und liefert offensichtlich trotz geringer Eindringtiefe (blau: 1-2 mm vs. rot und infrarot: 2-3 cm) Effekte, die noch näher untersucht werden sollten [5][6][7].…”

Section: Introductionunclassified

Blauer Laser in der Akupunktur – ein erster Review-Report

Litscher

Wang

Xie

et al. 2010

Schweiz Z Ganzheitsmed

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In der Akupunktur haben in den vergangenen 15 Jahren kleine, handliche Laser Einzug gehalten, mit denen Akupunkturpunkte bestrahlt werden, deren Effekte und therapeutische Wirkung aber in naturwissenschaftlichen Untersuchungen nach wie vor nicht eindeutig belegt sind. Die schmerzfreie, nicht invasive Laserakupunktur mit optischen Nadeln (infrarot + roter Laser) kann hingegen im Gehirn und in der Peripherie spezifische, reproduzierbare VerÄnderungen induzieren. Dies Äussert sich in Verschiebungen von unterschiedlichen Parametern, wie beispielsweise der BlutstrÖmungsgeschwindigkeit, und kann erstmals mit Methoden der High-TechAkupunktur® objektiviert werden. Ziel des vorliegenden Review-Reports ist es, die bislang vorliegenden experimentellen Messungen zur Laserstimulation mit einem blauen Laser zusammenzufassen. Diese blaue Lasernadelstrahlung (405 nm) erzeugt – wie in Grazer Pilotstudien festgestellt wurde – spezifische Effekte, die sich unter anderem in einem nadelÄhnlichen De-Qi-GefÜhl Äussern kÖnnen. Mit dem neuen Verfahren kÖnnte sich eine sinnvolle ErgÄnzung zur Nadelakupunktur fÜr Kinder oder PatientInnen mit Nadelangst ergeben, wobei die klinischen Anwendungen in Bezug auf die blaue Laserakupunktur erst ausgelotet werden mÜssen.

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Effects of Low Power Laser Irradiation on Intracellular Calcium and Histamine Release in RBL‐2H3 Mast Cells

Cited by 78 publications

References 34 publications

Regulation of Cellular Molecular Signaling by Light (Invited Paper)

Regulation of Cellular Molecular Signaling by Light (Invited Paper)

Fluid shear stress-induced cytosolic calcium signalling and degranulation dynamics in mast cells

Blauer Laser in der Akupunktur – ein erster Review-Report

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