Fluid shear stress-induced cytosolic calcium signalling and degranulation dynamics in mast cells

Wei, Fei; Shi, Xiaomin; Chen, Jiyao; Zhou, Leyuan

doi:10.1042/cbr20120004

Cited by 7 publications

(5 citation statements)

References 26 publications

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“…The reason behind frequency-displacement dependent transfection remains elusive; however, increased cytosolic Ca 2+ transients and enhanced Ca 2+ oscillations have been observed with increasing pacing frequency in adult mouse myocytes [30]. Similarly, shear stress induced cytosolic Ca 2+ transients are demonstrated in different cell types [31], [32]. The frequency-displacement dependent transfection we observe may depend on the cytosolic Ca 2+ oscillations, which is reported to interfere with the transfection and gene expression of cells [33], [34].…”

Section: Discussionmentioning

confidence: 60%

Enhanced Nanomagnetic Gene Transfection of Human Prenatal Cardiac Progenitor Cells and Adult Cardiomyocytes

2013

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Magnetic nanoparticle-based gene transfection has been shown to be an effective, non-viral technique for delivery of both plasmid DNA and siRNA into cells in culture. It has several advantages over other non-viral delivery techniques, such as short transfection times and high cell viability. These advantages have been demonstrated in a number of primary cells and cell lines. Here we report that oscillating magnet array-based nanomagnetic transfection significantly improves transfection efficiency in both human prenatal cardiac progenitor cells and adult cardiomyocytes when compared to static magnetofection, cationic lipid reagents and electroporation, while maintaining high cell viability. In addition, transfection of adult cardiomyocytes was improved further by seeding the cells onto Collagen I-coated plates, with transfection efficiencies of up to 49% compared to 24% with lipid reagents and 19% with electroporation. These results demonstrate that oscillating nanomagnetic transfection far outperforms other non-viral transfection techniques in these important cells.

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Section: Discussionmentioning

confidence: 60%

Enhanced Nanomagnetic Gene Transfection of Human Prenatal Cardiac Progenitor Cells and Adult Cardiomyocytes

2013

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“…Nevertheless, mechanotransduction corresponds to the most studied operating mode, experimentally or numerically. Needle manipulation in acupuncture can cause degranulation of mastocytes upon exposure to mechanical stress [7][8][9].…”

Section: Acupointmentioning

confidence: 99%

A Biological Model of Acupuncture and its Derived Mathematical Modeling and Simulations

Thiriet

Deleuze

Sheu

2015

Commun. Comput. Phys.

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Abstract. (Aims) Acupuncture was employed since 2 millenaries, but the underlying mechanisms are not globally handled. The present study is aimed at proposing an explanation by pointing out involved processes and a convincing modeling to demonstrate its efficiency when carried out by trained practitioners. (Method) In the absence of global knowledge of any mechanism explaining the acupuncture process, a biological model is first developed, based on stimulation in a given domain around the needle tip of a proper mastocyte population by a mechanical stress, electrical, electromagnetic, or heat field. Whatever the type of mechanical or physical stimuli, mastocytes degranulate. Released messengers either facilitate the transfer of main mediators, or target their cognate receptors of local nerve terminals or after being conveyed by blood their receptors on cerebral cells. Signaling to the brain is fast by nervous impulses and delayed by circulating messengers that nevertheless distribute preferentially in the brain region of interest due to hyperemia. The process is self-sustained due to mastocyte chemotaxis from the nearby dense microcirculatory circuit and surrounding mastocyte pools, which are inadequate for acupuncture, but serve as a signal relay. A simple mathematical model is solved analytically. Numerical simulations are also carried out using the finite element method with mesh adaptivity. (Results) The analytical solution of the simple mathematical model demonstrates the conditions filled by a mastocyte population to operate efficiently. A theorem gives the blow-up condition. This analytical solution serves for validation of numerical experiments. Numerical simulations show that when the needle is positioned in the periphery of the acupoint or outside it, the response is too weak. This explains why a long training is necessary as the needle implantation requires a precision with a magnitude of the order of 1 mm. (Conclusion) The acupoint must contain a highly concentrated population of mastocytes (e.g., very-high-amplitude, small-width Gaussian distribution) to get an initial proper response. Permanent signaling is provided by chemotaxis and continuous recruitment of mastocytes. Therefore, the density and distribution of mastocytes are crucial factors for efficient acupuncture as well as availability of circulating and neighboring pools of mastocytes. AMS subject classifications: 76M10, 92C10, 92C17, 92C50

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“…Local mechanical forces can trigger the activation of mechanoresponsive proteins on the cell surface [14,18] so that Ca ++ is allowed to enter the cytosol via pressure and shear stress gated ion channels. Simulations are carried out by considering fixed cells and no-slip boundary condition prescribed at the cell surface.…”

Section: Resultsmentioning

confidence: 99%

On Three-Dimensional ALE Finite Element Model For Simulating Interstitial Medium Deformation in the Presence of a Moving Needle

Deleuze

Thiriet

Sheu

2016

Advances in Computational Fluid-Structure Interaction and Flow Simulation

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The effects of inserted needle on the subcutaneous interstitial flow is studied. A goal is to describe the physical stress affecting cells during acupuncture needling. The convective Brinkman equations are considered to describe the flow through a fibrous medium. Three-dimensional simulations are carried out employing an ALE finite element model. Numerical studies illustrate the acute physical stress developed by the implantation of a needle.

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Fluid shear stress-induced cytosolic calcium signalling and degranulation dynamics in mast cells

Cited by 7 publications

References 26 publications

Enhanced Nanomagnetic Gene Transfection of Human Prenatal Cardiac Progenitor Cells and Adult Cardiomyocytes

Enhanced Nanomagnetic Gene Transfection of Human Prenatal Cardiac Progenitor Cells and Adult Cardiomyocytes

A Biological Model of Acupuncture and its Derived Mathematical Modeling and Simulations

On Three-Dimensional ALE Finite Element Model For Simulating Interstitial Medium Deformation in the Presence of a Moving Needle

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