Effects of Magnesium Sulfate on Tissue Lactate and Malondialdehyde Levels after Cerebral Ischemia

Abstract

In the present study, the effects of magnesium sulfate on tissue lactate and malondialdehyde (MDA) levels after cerebral ischemia in rabbits were studied. The rabbits were divided equally into three groups. Group 1 (n = 8) was the sham-operated control group, in group 2 (n = 8) only cerebral ischemia was induced by clamping bilaterally the common carotid arteries for 60 min, and in group 3 (n = 8) magnesium sulfate was administered at a dose of 100 mg/kg i.v. within 5 min after opening the clamps. In group 1 EEG recordings were obtained immediately and 60 and 120 min after craniectomy. In groups 2 and 3 EEG recordings were obtained immediately after craniectomy but before clamping and 60 min after clamping. One hour after opening the clamps and taking EEG recordings, brain cortices were resected, and the concentrations of lactate and MDA were determined using spectrophotometric/enzymatic and thiobarbituric acid methods, respectively. In all groups, there were significant differences between MDA and lactate levels (p < 0.05). There were no significant differences in lactate levels between groups 2 and 3 (p > 0.05), and also the preischemic EEG grades were the same in all groups. Preischemic and postischemic EEG values were significantly different (p < 0.05), and there were also significant differences between postischemic EEG grades in groups 2 and 3 (p < 0.05). There was a correlation between postischemic EEG grades and MDA and lactate levels. These results demonstrate that cerebral ischemia-reperfusion injury leads to an increase in brain tissue lactate and MDA levels, that magnesium sulfate suppresses the increase of MDA and lactate concentrations, and that magnesium sulfate treatment improves the EEG changes. The EEG grades correlated well with MDA and lactate levels.

Dynamic Alterations of Cerebral Pial Microcirculation During Experimental Subarachnoid Hemorrhage

“…It has been demonstrated that endothelin-1 was increased (Zimmermann and Seifert 1998;Sun et al 2000;Juvela 2002) and nitric oxide was decreased (Wolf et al 1998;Sun et al 2000;Pradilla, et al 2004) after SAH in different animal models and humans. In the present study, malondialdehyde, a product of oxygenic free radical metabolism (Bariskaner et al 2003), increased significantly after induction of SAH. It was also found in our study that the blood flow velocity was reduced and severe agglutinations of red blood cells occurred in the microvessels after SAH.…”

The effects of magnesium pretreatment on reperfusion injury during living donor liver transplantation

“…In another study [30], magnesium supplementation markedly inhibited lactate production and concomitant lipid peroxidation after cerebral ischemia. In this study, we observed higher blood lactate levels after reperfusion in the NS group with preoperative hypomagnesemia than those with normal preoperative magnesium levels.…”

Effects of magnesium sulfate on lipid peroxidation and blood pressure regulators in preeclampsia

“…Magnesium sulfate has been long known as a prophylactic convulsive treatment for PE [2,3]. Magnesium appears to be involved in free radicals bioavailability [9][10][11][12][13][14] and blood pressure regulation.…”

“…Mak et al [13] showed that magnesium gluconate had an antiradical and cytoprotective effects and inhibited lipid peroxidation in microsomal membranes obtained from cultured bovine aortic endothelial cells. In addition, magnesium deficiency has been associated with high free radicals and MDA levels [9,11], while magnesium supplementation reversed these effects [12][13][14][15]. These observations suggest that the effects of MgSO 4 treatment on MDA levels are associated with decreased free radicals.…”

“…However, the effects of MgSO 4 on lipid peroxidation and nitric oxide (NO) production have not been studied in PE. The fact that magnesium modulates free radicals, malondialdehyde 0009 (MDA), and NO production under different experimental conditions [9][10][11][12][13][14][15][16][17], suggests that the antihypertensive effects of this mineral in PE may involve changes in the regulation of lipid peroxidation and production of vasoactive factors that could act together in regulating hemodynamics in pregnancy. In this study, we have investigated the status of lipid peroxidation and serum levels of several vasoactive substances in PE pregnant women before and during treatment with MgSO 4 .…”