Effects of Magnetic Infrared Laser on Xylene-induced Acute Inflammation in Mice

Abstract

Abstract.[Purpose] The purpose of the present study was to investigate the effect of magnetic infrared laser (MIL) radiation on the xylene-induced acute inflammation.[Subjects] The subject animals were male ICR mice.[Methods] Mice were once irradiated (60s) by MIL at 1.33, 2.66 or 6.65 J/cm 2 of MIL, or treated with indomethacin or dexamethasone. Then xylene (0.03 ml) was topically applied to the anterior surface of the right ear to induced inflammation 1 h after irradiation with MIL. The changes in ear weights, histological profiles and histomorphometrical measurements of the ear were conducted upon sacrifice. All animals were sacrificed 2 h after xylene application. Results of MIL irradiation were compared to those of indomethacin and dexamethasone (15 mg/kg injected once intraperitoneally).[Results] Xylene application resulted in marked increases in xylene-insulted ear weights compared to that of the intact control. Thus, the differences between intact and insulted ears were also significantly increased. The histological characteristics of acute inflammation, such as severe vasodilation and edematous changes of skin, were detected in xylene-treated control ears with marked increase in the thickness of the ear tissues. However, these xylene-induced acute inflammatory changes were significantly and dose-dependently decreased by MIL irradiation. [Conclusion] We conclude that MIL therapy has a favorable effect in the reduction of the acute inflammatory responses induced by xylene application in mice.

Aqueous extract of Lannea microcarpa attenuates dextran sulphate-induced paw oedema and xylene-induced ear oedema in rodents

“…28 Xylene usually initiates inflammatory response resulting in oedema, warmth and redness of the skin of the ear of mice due to the release of histamine, kinin, fibrinolysin and substance P. [29][30][31][32] In this investigation, Lannea microcarpa extract (ALM) significantly inhibited xylene-induced ear oedema in a dose related manner and ultimately decreased irritation, oedema and erythema effects and so it could be suggested that the extract inhibited the activities of the proinflammatory mediators including histamine, kinins and fibrinolysin. Although the actual mechanism of action of Lannea microcarpa extract (ALM) was not demonstrated in this research, its anti-inflammatory effects may be attributed to one or more of the phytochemicals constituents such as flavonoids, steroids saponins and tannins which were found to be present in the extract.…”

Analgesic and Anti-inflammatory Activities of Ethanolic Extract of Clerodendrum inerme (L.) Gaertn.

“…Histopathologically topical application of xylene causes acute inflammation which is associated with severe vasodilation and edematous changes of skin (Ho et al, 2008;Puerta et al, 1996;Kim et al, 2007;Kou et al, 2003;Rotelli et al, 2003). According to present findings (Table 3), it can be claimed that the extract of C. inerme may exhibit anti-inflammatory activity through reducing vasodilation as well as improving edematous condition.…”

Effects of Magnetic Infrared Laser on Dinitrofluorobenzene-induced Contact Dermatitis in Mice

“…Because the allergic dermatitis evoked by DNFB shows phenomena similar to general inflammatory responses [3][4][5] and MIL has anti-inflammatory effects [16][17][18][19] , we considered that MIL irradiation would be ameliorated DNFB-induced contact dermatitis. In the present study, contact dermatitis was induced in mice by sensitization with DNP-OVA followed by DNFB challenge.…”

“…Direct evidence that MIL treatments ameliorate type I allergic dermatitis corresponds well to the clinical indications of MIL in the various inflammatory diseases [16][17][18] . These beneficial effects of MIL on the DNFB-induced dermatitis were considered as related with their antioxidant effects, 19,26) . However, the previously reported stimulation of the immune system 27,28) by MIL therapy may also be involved in the beneficial effects on allergic dermatitis activity detected in the present study because immunomodulatory agents can reduce inflammation 29) .…”

“…Because magnetic infrared laser (MIL) therapy has also been used in the treatment of various inflammatory diseases [16][17][18][19] , we hypothesized that MIL would ameliorate DNFB-induced contact dermatitis, because this allergic dermatitis also shows phenomena similar to general inflammatory responses [3][4][5] . When the surface of the skin is inflamed, cracked or raw, initial application of DNFB induces stinging or burning sensations.…”